Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines

Occupational exposure to the arylamines benzidine and β-naphthylamine increase bladder cancer risk up to 100-fold, making them some of the most powerful human carcinogens. We hypothesize that tumors arising in people with occupational exposures have different patterns of gene expression than histolo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ramya T. Kolli, Zongli Xu, Vijayalakshmi Panduri, Jack A. Taylor
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/8b08d0d5540b4a80bee2ae67ba04a2df
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8b08d0d5540b4a80bee2ae67ba04a2df
record_format	dspace
spelling	oai:doaj.org-article:8b08d0d5540b4a80bee2ae67ba04a2df2021-11-22T01:10:39ZDifferential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines2314-614110.1155/2021/2624433https://doaj.org/article/8b08d0d5540b4a80bee2ae67ba04a2df2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/2624433https://doaj.org/toc/2314-6141Occupational exposure to the arylamines benzidine and β-naphthylamine increase bladder cancer risk up to 100-fold, making them some of the most powerful human carcinogens. We hypothesize that tumors arising in people with occupational exposures have different patterns of gene expression than histologically similar tumors from people without such exposures. In a case-case study, we compare gene expression in 22 formalin-fixed paraffin-embedded (FFPE) bladder tumors from men with high-level occupational exposure to arylamines to that in 26 FFPE bladder tumors from men without such exposure. Gene expression analysis was performed on the NanoString nCounter system using a PanCancer Progression Panel comprised of 740 cancer progression-related genes and a custom panel of 69 arylamine- and bladder cancer-related genes which were chosen from in vitro studies. Although fold differences were small, there was evidence of differential expression by exposure status for 17 genes from the Progression Panel and 4 genes from the custom panel. In total, 10 genes showed dose-response association at a p<0.01, of which 4 genes (CD46, NR4A1, BAX, and YWHAZ) passed a false discovery rate (FDR) q value cutoff of 0.05 but were not significant after Bonferroni correction. Overall, we find limited evidence for differentially expressed genes in pathways related to DNA damage signaling and epithelial-to-mesenchymal transition (EMT).Ramya T. KolliZongli XuVijayalakshmi PanduriJack A. TaylorHindawi LimitedarticleMedicineRENBioMed Research International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Ramya T. Kolli Zongli Xu Vijayalakshmi Panduri Jack A. Taylor Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines
description	Occupational exposure to the arylamines benzidine and β-naphthylamine increase bladder cancer risk up to 100-fold, making them some of the most powerful human carcinogens. We hypothesize that tumors arising in people with occupational exposures have different patterns of gene expression than histologically similar tumors from people without such exposures. In a case-case study, we compare gene expression in 22 formalin-fixed paraffin-embedded (FFPE) bladder tumors from men with high-level occupational exposure to arylamines to that in 26 FFPE bladder tumors from men without such exposure. Gene expression analysis was performed on the NanoString nCounter system using a PanCancer Progression Panel comprised of 740 cancer progression-related genes and a custom panel of 69 arylamine- and bladder cancer-related genes which were chosen from in vitro studies. Although fold differences were small, there was evidence of differential expression by exposure status for 17 genes from the Progression Panel and 4 genes from the custom panel. In total, 10 genes showed dose-response association at a p<0.01, of which 4 genes (CD46, NR4A1, BAX, and YWHAZ) passed a false discovery rate (FDR) q value cutoff of 0.05 but were not significant after Bonferroni correction. Overall, we find limited evidence for differentially expressed genes in pathways related to DNA damage signaling and epithelial-to-mesenchymal transition (EMT).
format	article
author	Ramya T. Kolli Zongli Xu Vijayalakshmi Panduri Jack A. Taylor
author_facet	Ramya T. Kolli Zongli Xu Vijayalakshmi Panduri Jack A. Taylor
author_sort	Ramya T. Kolli
title	Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines
title_short	Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines
title_full	Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines
title_fullStr	Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines
title_full_unstemmed	Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines
title_sort	differential gene expression in bladder tumors from workers occupationally exposed to arylamines
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/8b08d0d5540b4a80bee2ae67ba04a2df
work_keys_str_mv	AT ramyatkolli differentialgeneexpressioninbladdertumorsfromworkersoccupationallyexposedtoarylamines AT zonglixu differentialgeneexpressioninbladdertumorsfromworkersoccupationallyexposedtoarylamines AT vijayalakshmipanduri differentialgeneexpressioninbladdertumorsfromworkersoccupationallyexposedtoarylamines AT jackataylor differentialgeneexpressioninbladdertumorsfromworkersoccupationallyexposedtoarylamines
_version_	1718418352324476928

Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines

Ejemplares similares