Tick-box for 3'-end formation of mitochondrial transcripts in Ixodida, basal chelicerates and Drosophila.

According to the tRNA punctuation model, the mitochondrial genome (mtDNA) of mammals and arthropods is transcribed as large polycistronic precursors that are maturated by endonucleolytic cleavage at tRNA borders and RNA polyadenylation. Starting from the newly sequenced mtDNA of Ixodes ricinus and u...

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Autores principales:	Matteo Montagna, Davide Sassera, Francesca Griggio, Sara Epis, Claudio Bandi, Carmela Gissi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b0f66572e28442d86b1f129f6d63d7c
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spelling	oai:doaj.org-article:8b0f66572e28442d86b1f129f6d63d7c2021-11-18T08:11:56ZTick-box for 3'-end formation of mitochondrial transcripts in Ixodida, basal chelicerates and Drosophila.1932-620310.1371/journal.pone.0047538https://doaj.org/article/8b0f66572e28442d86b1f129f6d63d7c2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23077630/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203According to the tRNA punctuation model, the mitochondrial genome (mtDNA) of mammals and arthropods is transcribed as large polycistronic precursors that are maturated by endonucleolytic cleavage at tRNA borders and RNA polyadenylation. Starting from the newly sequenced mtDNA of Ixodes ricinus and using a combination of mitogenomics and transcriptional analyses, we found that in all currently-sequenced tick lineages (Prostriata, Metastriata and Argasidae) the 3'-end of the polyadenylated nad1 and rrnL transcripts does not follow the tRNA punctuation model and is located upstream of a degenerate 17-bp DNA motif. A slightly different motif is also present downstream the 3'-end of nad1 transcripts in the primitive chelicerate Limulus polyphemus and in Drosophila species, indicating the ancient origin and the evolutionary conservation of this motif in arthropods. The transcriptional analyses suggest that this motif directs the 3'-end formation of the nad1/rrnL mature RNAs, likely working as a transcription termination signal or a processing signal of precursor transcripts. Moreover, as most regulatory elements, this motif is characterized by a taxon-specific evolution. Although this signal is not exclusive of ticks, making a play on words it has been named "Tick-Box", since it is a check mark that has to be verified for the 3'-end formation of some mt transcripts, and its consensus sequence has been here carefully characterized in ticks. Indeed, in the whole mtDNA of all ticks, the Tick-Box is always present downstream of nad1 and rrnL, mainly in non-coding regions (NCRs) and occasionally within trnL(CUN). However, some metastriates present a third Tick-Box at an intriguing site--inside the small NCR located at one end of a 3.4 kb translocated region, the other end of which exhibits the nad1 Tick-Box--hinting that this motif could have been involved in metastriate gene order rearrangements.Matteo MontagnaDavide SasseraFrancesca GriggioSara EpisClaudio BandiCarmela GissiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e47538 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Matteo Montagna Davide Sassera Francesca Griggio Sara Epis Claudio Bandi Carmela Gissi Tick-box for 3'-end formation of mitochondrial transcripts in Ixodida, basal chelicerates and Drosophila.
description	According to the tRNA punctuation model, the mitochondrial genome (mtDNA) of mammals and arthropods is transcribed as large polycistronic precursors that are maturated by endonucleolytic cleavage at tRNA borders and RNA polyadenylation. Starting from the newly sequenced mtDNA of Ixodes ricinus and using a combination of mitogenomics and transcriptional analyses, we found that in all currently-sequenced tick lineages (Prostriata, Metastriata and Argasidae) the 3'-end of the polyadenylated nad1 and rrnL transcripts does not follow the tRNA punctuation model and is located upstream of a degenerate 17-bp DNA motif. A slightly different motif is also present downstream the 3'-end of nad1 transcripts in the primitive chelicerate Limulus polyphemus and in Drosophila species, indicating the ancient origin and the evolutionary conservation of this motif in arthropods. The transcriptional analyses suggest that this motif directs the 3'-end formation of the nad1/rrnL mature RNAs, likely working as a transcription termination signal or a processing signal of precursor transcripts. Moreover, as most regulatory elements, this motif is characterized by a taxon-specific evolution. Although this signal is not exclusive of ticks, making a play on words it has been named "Tick-Box", since it is a check mark that has to be verified for the 3'-end formation of some mt transcripts, and its consensus sequence has been here carefully characterized in ticks. Indeed, in the whole mtDNA of all ticks, the Tick-Box is always present downstream of nad1 and rrnL, mainly in non-coding regions (NCRs) and occasionally within trnL(CUN). However, some metastriates present a third Tick-Box at an intriguing site--inside the small NCR located at one end of a 3.4 kb translocated region, the other end of which exhibits the nad1 Tick-Box--hinting that this motif could have been involved in metastriate gene order rearrangements.
format	article
author	Matteo Montagna Davide Sassera Francesca Griggio Sara Epis Claudio Bandi Carmela Gissi
author_facet	Matteo Montagna Davide Sassera Francesca Griggio Sara Epis Claudio Bandi Carmela Gissi
author_sort	Matteo Montagna
title	Tick-box for 3'-end formation of mitochondrial transcripts in Ixodida, basal chelicerates and Drosophila.
title_short	Tick-box for 3'-end formation of mitochondrial transcripts in Ixodida, basal chelicerates and Drosophila.
title_full	Tick-box for 3'-end formation of mitochondrial transcripts in Ixodida, basal chelicerates and Drosophila.
title_fullStr	Tick-box for 3'-end formation of mitochondrial transcripts in Ixodida, basal chelicerates and Drosophila.
title_full_unstemmed	Tick-box for 3'-end formation of mitochondrial transcripts in Ixodida, basal chelicerates and Drosophila.
title_sort	tick-box for 3'-end formation of mitochondrial transcripts in ixodida, basal chelicerates and drosophila.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/8b0f66572e28442d86b1f129f6d63d7c
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Tick-box for 3'-end formation of mitochondrial transcripts in Ixodida, basal chelicerates and Drosophila.

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