Five colour variants of bright luminescent protein for real-time multicolour bioimaging

The use of luminescence, rather than fluorescence, for imaging is advantageous in situations where laser illumination should be avoided. Here the authors make five colour variants of bright luminescent proteins based on FRET between NLuc and fluorescent proteins, and show their utility as Ca2+indica...

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Autores principales: Kazushi Suzuki, Taichi Kimura, Hajime Shinoda, Guirong Bai, Matthew J. Daniels, Yoshiyuki Arai, Masahiro Nakano, Takeharu Nagai
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/8b1143997ccc40c4b902738f03eeb3a6
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Sumario:The use of luminescence, rather than fluorescence, for imaging is advantageous in situations where laser illumination should be avoided. Here the authors make five colour variants of bright luminescent proteins based on FRET between NLuc and fluorescent proteins, and show their utility as Ca2+indicators.