Incremental Experience in In Vitro Primary Culture of Human Pulmonary Arterial Endothelial Cells Harvested from <i>Swan-Ganz</i> Pulmonary Arterial Catheters

Pulmonary arterial hypertension (PAH) is a devastating condition affecting the pulmonary microvascular wall and endothelium, resulting in their partial or total obstruction. Despite a combination of expensive vasodilatory therapies, mortality remains high. Personalized therapeutic approaches, based...

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Autores principales:	Birger Tielemans, Leanda Stoian, Allard Wagenaar, Mathias Leys, Catharina Belge, Marion Delcroix, Rozenn Quarck
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	pulmonary arterial hypertension endothelial cells right heart catheterization diagnosis cell culture Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8b1bed3ac8f4423ead4e2143c31992aa
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spelling	oai:doaj.org-article:8b1bed3ac8f4423ead4e2143c31992aa2021-11-25T17:13:07ZIncremental Experience in In Vitro Primary Culture of Human Pulmonary Arterial Endothelial Cells Harvested from <i>Swan-Ganz</i> Pulmonary Arterial Catheters10.3390/cells101132292073-4409https://doaj.org/article/8b1bed3ac8f4423ead4e2143c31992aa2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3229https://doaj.org/toc/2073-4409Pulmonary arterial hypertension (PAH) is a devastating condition affecting the pulmonary microvascular wall and endothelium, resulting in their partial or total obstruction. Despite a combination of expensive vasodilatory therapies, mortality remains high. Personalized therapeutic approaches, based on access to patient material to unravel patient specificities, could move the field forward. An innovative technique involving harvesting pulmonary arterial endothelial cells (PAECs) at the time of diagnosis was recently described. The aim of the present study was to fine-tune the initial technique and to phenotype the evolution of PAECs in vitro subcultures. PAECs were harvested from <i>Swan-Ganz</i> pulmonary arterial catheters during routine diagnostic or follow up right heart catheterization. Collected PAECs were phenotyped by flow cytometry and immunofluorescence focusing on endothelial-specific markers. We highlight the ability to harvest patients’ PAECs and to maintain them for up to 7–12 subcultures. By tracking the endothelial phenotype, we observed that PAECs could maintain an endothelial phenotype for several weeks in culture. The present study highlights the unique opportunity to obtain homogeneous subcultures of primary PAECs from patients at diagnosis and follow-up. In addition, it opens promising perspectives regarding tailored precision medicine for patients suffering from rare pulmonary vascular diseases.Birger TielemansLeanda StoianAllard WagenaarMathias LeysCatharina BelgeMarion DelcroixRozenn QuarckMDPI AGarticlepulmonary arterial hypertensionendothelial cellsright heart catheterizationdiagnosiscell cultureBiology (General)QH301-705.5ENCells, Vol 10, Iss 3229, p 3229 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pulmonary arterial hypertension endothelial cells right heart catheterization diagnosis cell culture Biology (General) QH301-705.5
spellingShingle	pulmonary arterial hypertension endothelial cells right heart catheterization diagnosis cell culture Biology (General) QH301-705.5 Birger Tielemans Leanda Stoian Allard Wagenaar Mathias Leys Catharina Belge Marion Delcroix Rozenn Quarck Incremental Experience in In Vitro Primary Culture of Human Pulmonary Arterial Endothelial Cells Harvested from <i>Swan-Ganz</i> Pulmonary Arterial Catheters
description	Pulmonary arterial hypertension (PAH) is a devastating condition affecting the pulmonary microvascular wall and endothelium, resulting in their partial or total obstruction. Despite a combination of expensive vasodilatory therapies, mortality remains high. Personalized therapeutic approaches, based on access to patient material to unravel patient specificities, could move the field forward. An innovative technique involving harvesting pulmonary arterial endothelial cells (PAECs) at the time of diagnosis was recently described. The aim of the present study was to fine-tune the initial technique and to phenotype the evolution of PAECs in vitro subcultures. PAECs were harvested from <i>Swan-Ganz</i> pulmonary arterial catheters during routine diagnostic or follow up right heart catheterization. Collected PAECs were phenotyped by flow cytometry and immunofluorescence focusing on endothelial-specific markers. We highlight the ability to harvest patients’ PAECs and to maintain them for up to 7–12 subcultures. By tracking the endothelial phenotype, we observed that PAECs could maintain an endothelial phenotype for several weeks in culture. The present study highlights the unique opportunity to obtain homogeneous subcultures of primary PAECs from patients at diagnosis and follow-up. In addition, it opens promising perspectives regarding tailored precision medicine for patients suffering from rare pulmonary vascular diseases.
format	article
author	Birger Tielemans Leanda Stoian Allard Wagenaar Mathias Leys Catharina Belge Marion Delcroix Rozenn Quarck
author_facet	Birger Tielemans Leanda Stoian Allard Wagenaar Mathias Leys Catharina Belge Marion Delcroix Rozenn Quarck
author_sort	Birger Tielemans
title	Incremental Experience in In Vitro Primary Culture of Human Pulmonary Arterial Endothelial Cells Harvested from <i>Swan-Ganz</i> Pulmonary Arterial Catheters
title_short	Incremental Experience in In Vitro Primary Culture of Human Pulmonary Arterial Endothelial Cells Harvested from <i>Swan-Ganz</i> Pulmonary Arterial Catheters
title_full	Incremental Experience in In Vitro Primary Culture of Human Pulmonary Arterial Endothelial Cells Harvested from <i>Swan-Ganz</i> Pulmonary Arterial Catheters
title_fullStr	Incremental Experience in In Vitro Primary Culture of Human Pulmonary Arterial Endothelial Cells Harvested from <i>Swan-Ganz</i> Pulmonary Arterial Catheters
title_full_unstemmed	Incremental Experience in In Vitro Primary Culture of Human Pulmonary Arterial Endothelial Cells Harvested from <i>Swan-Ganz</i> Pulmonary Arterial Catheters
title_sort	incremental experience in in vitro primary culture of human pulmonary arterial endothelial cells harvested from <i>swan-ganz</i> pulmonary arterial catheters
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8b1bed3ac8f4423ead4e2143c31992aa
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Incremental Experience in In Vitro Primary Culture of Human Pulmonary Arterial Endothelial Cells Harvested from <i>Swan-Ganz</i> Pulmonary Arterial Catheters

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