High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles.

Improved detection of anti-carbohydrate antibodies is a need in clinical identification of biomarkers for cancer cells or pathogens. Here, we report a new ELISA approach for the detection of specific immunoglobulins (IgGs) against carbohydrates. Two nanometer gold glyconanoparticles bearing oligosac...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Fabrizio Chiodo, Marco Marradi, Boris Tefsen, Harm Snippe, Irma van Die, Soledad Penadés
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b2430224ce84b2abaf8bffba18c9b0d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8b2430224ce84b2abaf8bffba18c9b0d
record_format	dspace
spelling	oai:doaj.org-article:8b2430224ce84b2abaf8bffba18c9b0d2021-11-18T08:58:03ZHigh sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles.1932-620310.1371/journal.pone.0073027https://doaj.org/article/8b2430224ce84b2abaf8bffba18c9b0d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24014084/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Improved detection of anti-carbohydrate antibodies is a need in clinical identification of biomarkers for cancer cells or pathogens. Here, we report a new ELISA approach for the detection of specific immunoglobulins (IgGs) against carbohydrates. Two nanometer gold glyconanoparticles bearing oligosaccharide epitopes of HIV or Streptococcus pneumoniae were used as antigens to coat ELISA-plates. A ~3,000-fold improved detection of specific IgGs in mice immunized against S. pneumoniae respect to the well known BSA-glycoconjugate ELISA was achieved. Moreover, these multivalent glyconanoparticles have been employed in solid phase assays to detect the carbohydrate-dependent binding of human dendritic cells and the lectin DC-SIGN. Multivalent glyconanoparticles in ELISA provide a versatile, easy and highly sensitive method to detect and quantify the binding of glycan to proteins and to facilitate the identification of biomarkers.Fabrizio ChiodoMarco MarradiBoris TefsenHarm SnippeIrma van DieSoledad PenadésPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e73027 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fabrizio Chiodo Marco Marradi Boris Tefsen Harm Snippe Irma van Die Soledad Penadés High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles.
description	Improved detection of anti-carbohydrate antibodies is a need in clinical identification of biomarkers for cancer cells or pathogens. Here, we report a new ELISA approach for the detection of specific immunoglobulins (IgGs) against carbohydrates. Two nanometer gold glyconanoparticles bearing oligosaccharide epitopes of HIV or Streptococcus pneumoniae were used as antigens to coat ELISA-plates. A ~3,000-fold improved detection of specific IgGs in mice immunized against S. pneumoniae respect to the well known BSA-glycoconjugate ELISA was achieved. Moreover, these multivalent glyconanoparticles have been employed in solid phase assays to detect the carbohydrate-dependent binding of human dendritic cells and the lectin DC-SIGN. Multivalent glyconanoparticles in ELISA provide a versatile, easy and highly sensitive method to detect and quantify the binding of glycan to proteins and to facilitate the identification of biomarkers.
format	article
author	Fabrizio Chiodo Marco Marradi Boris Tefsen Harm Snippe Irma van Die Soledad Penadés
author_facet	Fabrizio Chiodo Marco Marradi Boris Tefsen Harm Snippe Irma van Die Soledad Penadés
author_sort	Fabrizio Chiodo
title	High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles.
title_short	High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles.
title_full	High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles.
title_fullStr	High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles.
title_full_unstemmed	High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles.
title_sort	high sensitive detection of carbohydrate binding proteins in an elisa-solid phase assay based on multivalent glyconanoparticles.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/8b2430224ce84b2abaf8bffba18c9b0d
work_keys_str_mv	AT fabriziochiodo highsensitivedetectionofcarbohydratebindingproteinsinanelisasolidphaseassaybasedonmultivalentglyconanoparticles AT marcomarradi highsensitivedetectionofcarbohydratebindingproteinsinanelisasolidphaseassaybasedonmultivalentglyconanoparticles AT boristefsen highsensitivedetectionofcarbohydratebindingproteinsinanelisasolidphaseassaybasedonmultivalentglyconanoparticles AT harmsnippe highsensitivedetectionofcarbohydratebindingproteinsinanelisasolidphaseassaybasedonmultivalentglyconanoparticles AT irmavandie highsensitivedetectionofcarbohydratebindingproteinsinanelisasolidphaseassaybasedonmultivalentglyconanoparticles AT soledadpenades highsensitivedetectionofcarbohydratebindingproteinsinanelisasolidphaseassaybasedonmultivalentglyconanoparticles
_version_	1718421095501004800

High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles.

Ejemplares similares