MicroRNA expression profiling after recurrent febrile seizures in rat and emerging role of miR-148a-3p/SYNJ1 axis

Abstract Febrile seizures (FSs) are common neurological disorders in both infants and children, although the precise underlying mechanism remains to be explored, especially in the expression pattern and function of microRNAs (miRNAs). In this report, we aimed to screen new potential miRNAs and exami...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jian Xu, Mingqiang Sun, Xiaodong Li, Lei Huang, Zhenzhong Gao, Jian Gao, Anmu Xie
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b25411fca094c28a31068236eecdf4d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8b25411fca094c28a31068236eecdf4d
record_format	dspace
spelling	oai:doaj.org-article:8b25411fca094c28a31068236eecdf4d2021-12-02T14:12:43ZMicroRNA expression profiling after recurrent febrile seizures in rat and emerging role of miR-148a-3p/SYNJ1 axis10.1038/s41598-020-79543-02045-2322https://doaj.org/article/8b25411fca094c28a31068236eecdf4d2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79543-0https://doaj.org/toc/2045-2322Abstract Febrile seizures (FSs) are common neurological disorders in both infants and children, although the precise underlying mechanism remains to be explored, especially in the expression pattern and function of microRNAs (miRNAs). In this report, we aimed to screen new potential miRNAs and examine the role of miR-148a-3p in hippocampal neurons in FS rats via Synaptojanin-1 (SYNJ1). Thirty rats were randomly divided into the normal and FS model groups, which were investigated by miRNA array. This process identified 31 differentially expressed (20 upregulated and 11 downregulated) miRNAs and potential miRNA target genes. In addition, hippocampal neurons were assigned into five groups for different transfections. Apoptosis was detected by TUNEL and flow cytometry. SYNJ1 was identified as a target gene of miR-148-3p. In vitro experiments revealed that inhibition of miR-148a-3p decreased neuronal cell apoptosis. Moreover, overexpression of miR-148a-3p resulted in activation of PI3K/Akt signaling pathway and the apoptosis of hippocampal neurons. MiR-148a-3p inhibitor could reverse the above events. Taken together, our data demonstrated that the hippocampal miRNA expression profiles of a rat model of FS provide a large database of candidate miRNAs and neuron-related target genes. Furthermore, miR-148a-3p acted as a apoptosis enhcaner via the activation of the SYNJ1/PI3K/Akt signaling pathway, highlighting a potential therapeutic target in the treatment of infants with hyperthermia-induced brain injury.Jian XuMingqiang SunXiaodong LiLei HuangZhenzhong GaoJian GaoAnmu XieNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jian Xu Mingqiang Sun Xiaodong Li Lei Huang Zhenzhong Gao Jian Gao Anmu Xie MicroRNA expression profiling after recurrent febrile seizures in rat and emerging role of miR-148a-3p/SYNJ1 axis
description	Abstract Febrile seizures (FSs) are common neurological disorders in both infants and children, although the precise underlying mechanism remains to be explored, especially in the expression pattern and function of microRNAs (miRNAs). In this report, we aimed to screen new potential miRNAs and examine the role of miR-148a-3p in hippocampal neurons in FS rats via Synaptojanin-1 (SYNJ1). Thirty rats were randomly divided into the normal and FS model groups, which were investigated by miRNA array. This process identified 31 differentially expressed (20 upregulated and 11 downregulated) miRNAs and potential miRNA target genes. In addition, hippocampal neurons were assigned into five groups for different transfections. Apoptosis was detected by TUNEL and flow cytometry. SYNJ1 was identified as a target gene of miR-148-3p. In vitro experiments revealed that inhibition of miR-148a-3p decreased neuronal cell apoptosis. Moreover, overexpression of miR-148a-3p resulted in activation of PI3K/Akt signaling pathway and the apoptosis of hippocampal neurons. MiR-148a-3p inhibitor could reverse the above events. Taken together, our data demonstrated that the hippocampal miRNA expression profiles of a rat model of FS provide a large database of candidate miRNAs and neuron-related target genes. Furthermore, miR-148a-3p acted as a apoptosis enhcaner via the activation of the SYNJ1/PI3K/Akt signaling pathway, highlighting a potential therapeutic target in the treatment of infants with hyperthermia-induced brain injury.
format	article
author	Jian Xu Mingqiang Sun Xiaodong Li Lei Huang Zhenzhong Gao Jian Gao Anmu Xie
author_facet	Jian Xu Mingqiang Sun Xiaodong Li Lei Huang Zhenzhong Gao Jian Gao Anmu Xie
author_sort	Jian Xu
title	MicroRNA expression profiling after recurrent febrile seizures in rat and emerging role of miR-148a-3p/SYNJ1 axis
title_short	MicroRNA expression profiling after recurrent febrile seizures in rat and emerging role of miR-148a-3p/SYNJ1 axis
title_full	MicroRNA expression profiling after recurrent febrile seizures in rat and emerging role of miR-148a-3p/SYNJ1 axis
title_fullStr	MicroRNA expression profiling after recurrent febrile seizures in rat and emerging role of miR-148a-3p/SYNJ1 axis
title_full_unstemmed	MicroRNA expression profiling after recurrent febrile seizures in rat and emerging role of miR-148a-3p/SYNJ1 axis
title_sort	microrna expression profiling after recurrent febrile seizures in rat and emerging role of mir-148a-3p/synj1 axis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8b25411fca094c28a31068236eecdf4d
work_keys_str_mv	AT jianxu micrornaexpressionprofilingafterrecurrentfebrileseizuresinratandemergingroleofmir148a3psynj1axis AT mingqiangsun micrornaexpressionprofilingafterrecurrentfebrileseizuresinratandemergingroleofmir148a3psynj1axis AT xiaodongli micrornaexpressionprofilingafterrecurrentfebrileseizuresinratandemergingroleofmir148a3psynj1axis AT leihuang micrornaexpressionprofilingafterrecurrentfebrileseizuresinratandemergingroleofmir148a3psynj1axis AT zhenzhonggao micrornaexpressionprofilingafterrecurrentfebrileseizuresinratandemergingroleofmir148a3psynj1axis AT jiangao micrornaexpressionprofilingafterrecurrentfebrileseizuresinratandemergingroleofmir148a3psynj1axis AT anmuxie micrornaexpressionprofilingafterrecurrentfebrileseizuresinratandemergingroleofmir148a3psynj1axis
_version_	1718391796376010752

MicroRNA expression profiling after recurrent febrile seizures in rat and emerging role of miR-148a-3p/SYNJ1 axis

Ejemplares similares