A change in the aggregation pathway of bovine serum albumin in the presence of arginine and its derivatives

Abstract Chemical chaperones including arginine and its derivatives are widely used by biochemists working on the design of agents, which are able to efficiently suppress protein aggregation. To elucidate the mechanisms of anti-aggregation activity of chemical chaperones, methods based on registrati...

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Autores principales:	Vera A. Borzova, Kira A. Markossian, Sergey Yu. Kleymenov, Boris I. Kurganov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b2b652c7b3648cfbc11615f6a194c30
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spelling	oai:doaj.org-article:8b2b652c7b3648cfbc11615f6a194c302021-12-02T11:52:43ZA change in the aggregation pathway of bovine serum albumin in the presence of arginine and its derivatives10.1038/s41598-017-04409-x2045-2322https://doaj.org/article/8b2b652c7b3648cfbc11615f6a194c302017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04409-xhttps://doaj.org/toc/2045-2322Abstract Chemical chaperones including arginine and its derivatives are widely used by biochemists working on the design of agents, which are able to efficiently suppress protein aggregation. To elucidate the mechanisms of anti-aggregation activity of chemical chaperones, methods based on registration of the increment in light scattering intensity must be supplemented with methods for direct detection of the portion of aggregated protein (γagg). For this purpose asymmetric flow field-flow fractionation was used in the present work. It was shown that heat-induced aggregation of bovine serum albumin (BSA) followed the kinetics of the reaction of the second order (0.1 M sodium phosphate buffer, pH 7.0, 70 °C). It was proposed to use R h vs γagg plots to characterize the aggregation pathway (R h is the hydrodynamic radius of the protein aggregates, which was calculated from the dynamic light scattering data). The changes in the shape of R h vs γagg plots in the presence of arginine, arginine amide and arginine ethyl ester are indicative of the changes in the aggregation pathway of BSA aggregation. A conclusion has been made that larger aggregates are formed in the presence of arginine hydrochloride and its derivatives.Vera A. BorzovaKira A. MarkossianSergey Yu. KleymenovBoris I. KurganovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vera A. Borzova Kira A. Markossian Sergey Yu. Kleymenov Boris I. Kurganov A change in the aggregation pathway of bovine serum albumin in the presence of arginine and its derivatives
description	Abstract Chemical chaperones including arginine and its derivatives are widely used by biochemists working on the design of agents, which are able to efficiently suppress protein aggregation. To elucidate the mechanisms of anti-aggregation activity of chemical chaperones, methods based on registration of the increment in light scattering intensity must be supplemented with methods for direct detection of the portion of aggregated protein (γagg). For this purpose asymmetric flow field-flow fractionation was used in the present work. It was shown that heat-induced aggregation of bovine serum albumin (BSA) followed the kinetics of the reaction of the second order (0.1 M sodium phosphate buffer, pH 7.0, 70 °C). It was proposed to use R h vs γagg plots to characterize the aggregation pathway (R h is the hydrodynamic radius of the protein aggregates, which was calculated from the dynamic light scattering data). The changes in the shape of R h vs γagg plots in the presence of arginine, arginine amide and arginine ethyl ester are indicative of the changes in the aggregation pathway of BSA aggregation. A conclusion has been made that larger aggregates are formed in the presence of arginine hydrochloride and its derivatives.
format	article
author	Vera A. Borzova Kira A. Markossian Sergey Yu. Kleymenov Boris I. Kurganov
author_facet	Vera A. Borzova Kira A. Markossian Sergey Yu. Kleymenov Boris I. Kurganov
author_sort	Vera A. Borzova
title	A change in the aggregation pathway of bovine serum albumin in the presence of arginine and its derivatives
title_short	A change in the aggregation pathway of bovine serum albumin in the presence of arginine and its derivatives
title_full	A change in the aggregation pathway of bovine serum albumin in the presence of arginine and its derivatives
title_fullStr	A change in the aggregation pathway of bovine serum albumin in the presence of arginine and its derivatives
title_full_unstemmed	A change in the aggregation pathway of bovine serum albumin in the presence of arginine and its derivatives
title_sort	change in the aggregation pathway of bovine serum albumin in the presence of arginine and its derivatives
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8b2b652c7b3648cfbc11615f6a194c30
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A change in the aggregation pathway of bovine serum albumin in the presence of arginine and its derivatives

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