Development and Validation of a Stability-Indicating HPLC Method for the Simultaneous Determination of trans-Resveratrol and cis-Resveratrol in an Injectable Solution

trans-Resveratrol, a phytochemical compound with antioxidant power and various therapeutic effects, such as cardioprotective, chemopreventive, and neuroprotective, among others, has disadvantages of poor solubility and limited stability, creating difficulties for the development of new strategies fo...

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Autores principales:	Esmeralda Mota-Lugo, Mariana Dolores-Hernández, Elvia A. Morales-Hipólito, Iris A. Blanco-Alcántara, Hugo Cuatecontzi-Flores, Raquel López-Arellano
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Analytical chemistry QD71-142
Acceso en línea:	https://doaj.org/article/8b2db3d839da41c7adc1c7189e7ea3ba
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spelling	oai:doaj.org-article:8b2db3d839da41c7adc1c7189e7ea3ba2021-11-22T01:10:10ZDevelopment and Validation of a Stability-Indicating HPLC Method for the Simultaneous Determination of trans-Resveratrol and cis-Resveratrol in an Injectable Solution2090-887310.1155/2021/8402157https://doaj.org/article/8b2db3d839da41c7adc1c7189e7ea3ba2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/8402157https://doaj.org/toc/2090-8873trans-Resveratrol, a phytochemical compound with antioxidant power and various therapeutic effects, such as cardioprotective, chemopreventive, and neuroprotective, among others, has disadvantages of poor solubility and limited stability, creating difficulties for the development of new strategies for its quantification. This study developed and validated an analytical stability method for trans-resveratrol by high-pressure liquid chromatography with photodiode-array detection (HPLC-PDA), which allowed its quantification in the presence of its degradation products. The quantification of trans-resveratrol occurred at a retention time of 2.6 min, with ammonium formate (10 mM, pH = 4)/acetonitrile, 70/30 v/v, as mobile phase. The validation met the ICH Q2 criteria of specificity, method linearity (2.8–4.2 μg/ml), precision and accuracy, robustness, quantification limit (0.176 μg/ml), and detection (0.058 μg/ml). As degradation products, cis-resveratrol was observed at 3.9 min, which could be resveratrone in 3.2 min and five unidentified products in 0.7, 1.0, 1.4, 1.8, and 5 min. Some solutions subjected to temperature stress of 40 and 60°C, UV light, and acidic and basic hydrolysis exhibited colour changes. An analytical method was obtained by HPLC-PDA, which allowed quantifying the stability of trans-resveratrol in a fast and specific manner in the presence of its degradation products.Esmeralda Mota-LugoMariana Dolores-HernándezElvia A. Morales-HipólitoIris A. Blanco-AlcántaraHugo Cuatecontzi-FloresRaquel López-ArellanoHindawi LimitedarticleAnalytical chemistryQD71-142ENJournal of Analytical Methods in Chemistry, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Analytical chemistry QD71-142
spellingShingle	Analytical chemistry QD71-142 Esmeralda Mota-Lugo Mariana Dolores-Hernández Elvia A. Morales-Hipólito Iris A. Blanco-Alcántara Hugo Cuatecontzi-Flores Raquel López-Arellano Development and Validation of a Stability-Indicating HPLC Method for the Simultaneous Determination of trans-Resveratrol and cis-Resveratrol in an Injectable Solution
description	trans-Resveratrol, a phytochemical compound with antioxidant power and various therapeutic effects, such as cardioprotective, chemopreventive, and neuroprotective, among others, has disadvantages of poor solubility and limited stability, creating difficulties for the development of new strategies for its quantification. This study developed and validated an analytical stability method for trans-resveratrol by high-pressure liquid chromatography with photodiode-array detection (HPLC-PDA), which allowed its quantification in the presence of its degradation products. The quantification of trans-resveratrol occurred at a retention time of 2.6 min, with ammonium formate (10 mM, pH = 4)/acetonitrile, 70/30 v/v, as mobile phase. The validation met the ICH Q2 criteria of specificity, method linearity (2.8–4.2 μg/ml), precision and accuracy, robustness, quantification limit (0.176 μg/ml), and detection (0.058 μg/ml). As degradation products, cis-resveratrol was observed at 3.9 min, which could be resveratrone in 3.2 min and five unidentified products in 0.7, 1.0, 1.4, 1.8, and 5 min. Some solutions subjected to temperature stress of 40 and 60°C, UV light, and acidic and basic hydrolysis exhibited colour changes. An analytical method was obtained by HPLC-PDA, which allowed quantifying the stability of trans-resveratrol in a fast and specific manner in the presence of its degradation products.
format	article
author	Esmeralda Mota-Lugo Mariana Dolores-Hernández Elvia A. Morales-Hipólito Iris A. Blanco-Alcántara Hugo Cuatecontzi-Flores Raquel López-Arellano
author_facet	Esmeralda Mota-Lugo Mariana Dolores-Hernández Elvia A. Morales-Hipólito Iris A. Blanco-Alcántara Hugo Cuatecontzi-Flores Raquel López-Arellano
author_sort	Esmeralda Mota-Lugo
title	Development and Validation of a Stability-Indicating HPLC Method for the Simultaneous Determination of trans-Resveratrol and cis-Resveratrol in an Injectable Solution
title_short	Development and Validation of a Stability-Indicating HPLC Method for the Simultaneous Determination of trans-Resveratrol and cis-Resveratrol in an Injectable Solution
title_full	Development and Validation of a Stability-Indicating HPLC Method for the Simultaneous Determination of trans-Resveratrol and cis-Resveratrol in an Injectable Solution
title_fullStr	Development and Validation of a Stability-Indicating HPLC Method for the Simultaneous Determination of trans-Resveratrol and cis-Resveratrol in an Injectable Solution
title_full_unstemmed	Development and Validation of a Stability-Indicating HPLC Method for the Simultaneous Determination of trans-Resveratrol and cis-Resveratrol in an Injectable Solution
title_sort	development and validation of a stability-indicating hplc method for the simultaneous determination of trans-resveratrol and cis-resveratrol in an injectable solution
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/8b2db3d839da41c7adc1c7189e7ea3ba
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Development and Validation of a Stability-Indicating HPLC Method for the Simultaneous Determination of trans-Resveratrol and cis-Resveratrol in an Injectable Solution

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