A pilot trial of intravital microscopy in the study of the tumor vasculature of patients with peritoneal carcinomatosis

Abstract Aberrancies in the tumor microvasculature limit the systemic delivery of anticancer agents, which impedes tumor response. Using human intravital microscopy (HIVM), we hypothesized that HIVM would be feasible in patients with peritoneal carcinomatosis (PC). During cytoreductive surgery with...

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Autores principales:	Emmanuel M. Gabriel, Minhyung Kim, Daniel T. Fisher, Catherine Mangum, Kristopher Attwood, Wenyan Ji, Debabrata Mukhopadhyay, Sanjay P. Bagaria, Matthew W. Robertson, Tri A. Dinh, Keith L. Knutson, Joseph J. Skitzki, Michael B. Wallace
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b34f51c24a64fa09479af5932207948
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spelling	oai:doaj.org-article:8b34f51c24a64fa09479af59322079482021-12-02T13:20:14ZA pilot trial of intravital microscopy in the study of the tumor vasculature of patients with peritoneal carcinomatosis10.1038/s41598-021-84430-32045-2322https://doaj.org/article/8b34f51c24a64fa09479af59322079482021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84430-3https://doaj.org/toc/2045-2322Abstract Aberrancies in the tumor microvasculature limit the systemic delivery of anticancer agents, which impedes tumor response. Using human intravital microscopy (HIVM), we hypothesized that HIVM would be feasible in patients with peritoneal carcinomatosis (PC). During cytoreductive surgery with hyperthermic intraperitoneal chemotherapy for PC, HIVM was performed in both tumor and non-tumor areas. The primary outcome was HIVM feasibility to measure vessel characteristics. We secondarily evaluated associations between HIVM vessel characteristics and oncologic outcomes (RECIST response to neoadjuvant therapy and disease-specific survival). Thirty patients with PC were enrolled. Nineteen patients (63.3%) received neoadjuvant therapy. HIVM was feasible in all patients. Compared to non-tumor (control) areas, PC areas had a lower density of functional vessels, higher proportion of non-functional vessels, smaller lumenal diameters, and lower blood flow velocity. Qualitative differences in these vessel characteristics were observed among patients who had partial response, stable disease, or progressive disease after receiving neoadjuvant therapy. However, no statistically significant relationships were found between HIVM vessel characteristics and oncologic outcomes. These novel findings comprise the first-in-human, real-time evidence of the microscopic differences between normal and tumor-associated vessels and form the basis for our larger, ongoing clinical trial appropriately powered to determine the clinical utility of HIVM (NCT03823144).Emmanuel M. GabrielMinhyung KimDaniel T. FisherCatherine MangumKristopher AttwoodWenyan JiDebabrata MukhopadhyaySanjay P. BagariaMatthew W. RobertsonTri A. DinhKeith L. KnutsonJoseph J. SkitzkiMichael B. WallaceNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emmanuel M. Gabriel Minhyung Kim Daniel T. Fisher Catherine Mangum Kristopher Attwood Wenyan Ji Debabrata Mukhopadhyay Sanjay P. Bagaria Matthew W. Robertson Tri A. Dinh Keith L. Knutson Joseph J. Skitzki Michael B. Wallace A pilot trial of intravital microscopy in the study of the tumor vasculature of patients with peritoneal carcinomatosis
description	Abstract Aberrancies in the tumor microvasculature limit the systemic delivery of anticancer agents, which impedes tumor response. Using human intravital microscopy (HIVM), we hypothesized that HIVM would be feasible in patients with peritoneal carcinomatosis (PC). During cytoreductive surgery with hyperthermic intraperitoneal chemotherapy for PC, HIVM was performed in both tumor and non-tumor areas. The primary outcome was HIVM feasibility to measure vessel characteristics. We secondarily evaluated associations between HIVM vessel characteristics and oncologic outcomes (RECIST response to neoadjuvant therapy and disease-specific survival). Thirty patients with PC were enrolled. Nineteen patients (63.3%) received neoadjuvant therapy. HIVM was feasible in all patients. Compared to non-tumor (control) areas, PC areas had a lower density of functional vessels, higher proportion of non-functional vessels, smaller lumenal diameters, and lower blood flow velocity. Qualitative differences in these vessel characteristics were observed among patients who had partial response, stable disease, or progressive disease after receiving neoadjuvant therapy. However, no statistically significant relationships were found between HIVM vessel characteristics and oncologic outcomes. These novel findings comprise the first-in-human, real-time evidence of the microscopic differences between normal and tumor-associated vessels and form the basis for our larger, ongoing clinical trial appropriately powered to determine the clinical utility of HIVM (NCT03823144).
format	article
author	Emmanuel M. Gabriel Minhyung Kim Daniel T. Fisher Catherine Mangum Kristopher Attwood Wenyan Ji Debabrata Mukhopadhyay Sanjay P. Bagaria Matthew W. Robertson Tri A. Dinh Keith L. Knutson Joseph J. Skitzki Michael B. Wallace
author_facet	Emmanuel M. Gabriel Minhyung Kim Daniel T. Fisher Catherine Mangum Kristopher Attwood Wenyan Ji Debabrata Mukhopadhyay Sanjay P. Bagaria Matthew W. Robertson Tri A. Dinh Keith L. Knutson Joseph J. Skitzki Michael B. Wallace
author_sort	Emmanuel M. Gabriel
title	A pilot trial of intravital microscopy in the study of the tumor vasculature of patients with peritoneal carcinomatosis
title_short	A pilot trial of intravital microscopy in the study of the tumor vasculature of patients with peritoneal carcinomatosis
title_full	A pilot trial of intravital microscopy in the study of the tumor vasculature of patients with peritoneal carcinomatosis
title_fullStr	A pilot trial of intravital microscopy in the study of the tumor vasculature of patients with peritoneal carcinomatosis
title_full_unstemmed	A pilot trial of intravital microscopy in the study of the tumor vasculature of patients with peritoneal carcinomatosis
title_sort	pilot trial of intravital microscopy in the study of the tumor vasculature of patients with peritoneal carcinomatosis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8b34f51c24a64fa09479af5932207948
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A pilot trial of intravital microscopy in the study of the tumor vasculature of patients with peritoneal carcinomatosis

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