DNA extraction columns contaminated with murine sequences.

Sequences of the novel gammaretrovirus, xenotropic murine leukemia virus-related virus (XMRV) have been described in human prostate cancer tissue, although the amounts of DNA are low. Furthermore, XMRV sequences and polytropic (p) murine leukemia viruses (MLVs) have been reported in patients with ch...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Otto Erlwein, Mark J Robinson, Simon Dustan, Jonathan Weber, Steve Kaye, Myra O McClure
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b37d89dc42b45a9ac0d58aada8447b8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8b37d89dc42b45a9ac0d58aada8447b8
record_format	dspace
spelling	oai:doaj.org-article:8b37d89dc42b45a9ac0d58aada8447b82021-11-18T06:47:40ZDNA extraction columns contaminated with murine sequences.1932-620310.1371/journal.pone.0023484https://doaj.org/article/8b37d89dc42b45a9ac0d58aada8447b82011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21876752/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Sequences of the novel gammaretrovirus, xenotropic murine leukemia virus-related virus (XMRV) have been described in human prostate cancer tissue, although the amounts of DNA are low. Furthermore, XMRV sequences and polytropic (p) murine leukemia viruses (MLVs) have been reported in patients with chronic fatigue syndrome (CFS). In assessing the prevalence of XMRV in prostate cancer tissue samples we discovered that eluates from naïve DNA purification columns, when subjected to PCR with primers designed to detect genomic mouse DNA contamination, occasionally gave rise to amplification products. Further PCR analysis, using primers to detect XMRV, revealed sequences derived from XMRV and pMLVs from mouse and human DNA and DNA of unspecified origin. Thus, DNA purification columns can present problems when used to detect minute amounts of DNA targets by highly sensitive amplification techniques.Otto ErlweinMark J RobinsonSimon DustanJonathan WeberSteve KayeMyra O McClurePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e23484 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Otto Erlwein Mark J Robinson Simon Dustan Jonathan Weber Steve Kaye Myra O McClure DNA extraction columns contaminated with murine sequences.
description	Sequences of the novel gammaretrovirus, xenotropic murine leukemia virus-related virus (XMRV) have been described in human prostate cancer tissue, although the amounts of DNA are low. Furthermore, XMRV sequences and polytropic (p) murine leukemia viruses (MLVs) have been reported in patients with chronic fatigue syndrome (CFS). In assessing the prevalence of XMRV in prostate cancer tissue samples we discovered that eluates from naïve DNA purification columns, when subjected to PCR with primers designed to detect genomic mouse DNA contamination, occasionally gave rise to amplification products. Further PCR analysis, using primers to detect XMRV, revealed sequences derived from XMRV and pMLVs from mouse and human DNA and DNA of unspecified origin. Thus, DNA purification columns can present problems when used to detect minute amounts of DNA targets by highly sensitive amplification techniques.
format	article
author	Otto Erlwein Mark J Robinson Simon Dustan Jonathan Weber Steve Kaye Myra O McClure
author_facet	Otto Erlwein Mark J Robinson Simon Dustan Jonathan Weber Steve Kaye Myra O McClure
author_sort	Otto Erlwein
title	DNA extraction columns contaminated with murine sequences.
title_short	DNA extraction columns contaminated with murine sequences.
title_full	DNA extraction columns contaminated with murine sequences.
title_fullStr	DNA extraction columns contaminated with murine sequences.
title_full_unstemmed	DNA extraction columns contaminated with murine sequences.
title_sort	dna extraction columns contaminated with murine sequences.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/8b37d89dc42b45a9ac0d58aada8447b8
work_keys_str_mv	AT ottoerlwein dnaextractioncolumnscontaminatedwithmurinesequences AT markjrobinson dnaextractioncolumnscontaminatedwithmurinesequences AT simondustan dnaextractioncolumnscontaminatedwithmurinesequences AT jonathanweber dnaextractioncolumnscontaminatedwithmurinesequences AT stevekaye dnaextractioncolumnscontaminatedwithmurinesequences AT myraomcclure dnaextractioncolumnscontaminatedwithmurinesequences
_version_	1718424363610406912

DNA extraction columns contaminated with murine sequences.

Ejemplares similares