Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid.

The ovarian, or coelomic, fluid that is released with the egg mass of many fishes is increasingly found to play an important role in several biological processes crucial for reproductive success. These include maintenance of oocyte fertility and developmental competence, prolonging of sperm motility...

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Autores principales:	Sheri L Johnson, Marsha Villarroel, Patrice Rosengrave, Alan Carne, Torsten Kleffmann, P Mark Lokman, Neil J Gemmell
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b484866d6b44eaf8561eb98249b75b7
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spelling	oai:doaj.org-article:8b484866d6b44eaf8561eb98249b75b72021-11-25T06:06:09ZProteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid.1932-620310.1371/journal.pone.0104155https://doaj.org/article/8b484866d6b44eaf8561eb98249b75b72014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25089903/?tool=EBIhttps://doaj.org/toc/1932-6203The ovarian, or coelomic, fluid that is released with the egg mass of many fishes is increasingly found to play an important role in several biological processes crucial for reproductive success. These include maintenance of oocyte fertility and developmental competence, prolonging of sperm motility, and enhancing sperm swimming speed. Here we examined if and how the proteome of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid varied among females and then sought to examine the composition of this fluid. Ovarian fluid in chinook salmon was analyzed using 1D SDS PAGE and LC-MS/MS tryptic digest screened against Mascot and Sequest databases. We found marked differences in the number and concentrations of proteins in salmon ovarian fluid across different females. A total of 174 proteins were identified in ovarian fluid, 47 of which were represented by six or more peptides, belonging to one of six Gene Ontology pathways. The response to chemical stimulus and response to hypoxia pathways were best represented, accounting for 26 of the 174 proteins. The current data set provides a resource that furthers our understanding of those factors that influence successful egg production and fertilisation in salmonids and other species.Sheri L JohnsonMarsha VillarroelPatrice RosengraveAlan CarneTorsten KleffmannP Mark LokmanNeil J GemmellPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e104155 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sheri L Johnson Marsha Villarroel Patrice Rosengrave Alan Carne Torsten Kleffmann P Mark Lokman Neil J Gemmell Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid.
description	The ovarian, or coelomic, fluid that is released with the egg mass of many fishes is increasingly found to play an important role in several biological processes crucial for reproductive success. These include maintenance of oocyte fertility and developmental competence, prolonging of sperm motility, and enhancing sperm swimming speed. Here we examined if and how the proteome of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid varied among females and then sought to examine the composition of this fluid. Ovarian fluid in chinook salmon was analyzed using 1D SDS PAGE and LC-MS/MS tryptic digest screened against Mascot and Sequest databases. We found marked differences in the number and concentrations of proteins in salmon ovarian fluid across different females. A total of 174 proteins were identified in ovarian fluid, 47 of which were represented by six or more peptides, belonging to one of six Gene Ontology pathways. The response to chemical stimulus and response to hypoxia pathways were best represented, accounting for 26 of the 174 proteins. The current data set provides a resource that furthers our understanding of those factors that influence successful egg production and fertilisation in salmonids and other species.
format	article
author	Sheri L Johnson Marsha Villarroel Patrice Rosengrave Alan Carne Torsten Kleffmann P Mark Lokman Neil J Gemmell
author_facet	Sheri L Johnson Marsha Villarroel Patrice Rosengrave Alan Carne Torsten Kleffmann P Mark Lokman Neil J Gemmell
author_sort	Sheri L Johnson
title	Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid.
title_short	Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid.
title_full	Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid.
title_fullStr	Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid.
title_full_unstemmed	Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid.
title_sort	proteomic analysis of chinook salmon (oncorhynchus tshawytscha) ovarian fluid.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/8b484866d6b44eaf8561eb98249b75b7
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Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid.

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