The cytotoxic effects of lipidic formulated gold porphyrin nanoparticles for the treatment of neuroblastoma

Puiyan Lee1, Yifan Zhu1, Jessie J Yan2, Raymond WY Sun2, Wei Hao1, Xuelai Liu1, Chi-Ming Che2, Kenneth KY Wong11Department of Surgery, Li Ka Shing Faculty of Medicine, 2Department of Chemistry and Open Laboratory of Chemical Biology of the Institute of Molecular Technology for Drug Discovery and Syn...

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Autores principales:	Puiyan Lee, Yifan Zhu, Jessie J Yan, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medical technology R855-855.5 Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/8b487cfabb64448d99edc1f440bdf6ed
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spelling	oai:doaj.org-article:8b487cfabb64448d99edc1f440bdf6ed2021-12-02T02:52:29ZThe cytotoxic effects of lipidic formulated gold porphyrin nanoparticles for the treatment of neuroblastoma1177-8903https://doaj.org/article/8b487cfabb64448d99edc1f440bdf6ed2010-06-01T00:00:00Zhttp://www.dovepress.com/the-cytotoxic-effects-of-lipidic-formulated-gold-porphyrin-nanoparticl-a4606https://doaj.org/toc/1177-8903Puiyan Lee1, Yifan Zhu1, Jessie J Yan2, Raymond WY Sun2, Wei Hao1, Xuelai Liu1, Chi-Ming Che2, Kenneth KY Wong11Department of Surgery, Li Ka Shing Faculty of Medicine, 2Department of Chemistry and Open Laboratory of Chemical Biology of the Institute of Molecular Technology for Drug Discovery and Synthesis, The University of Hong Kong, Hong Kong Objective: Nanotechnology has been identified as a promising platform in the improvement of the design and development of drug delivery systems. In the present study we investigated the potential of lipidic nanoparticles consisting of gold porphyrin for the treatment of neuroblastoma.Materials and methods: To characterize the size of the lipidic gold porphyrin nanoparticles, we used transmission electron microscopy (TEM). The in vitro cytotoxic effect on neuroblastoma activity was examined using XTT cell proliferation assay, then IC50 values were calculated. In vivo safety and toxicity were studied using intraperitoneal injection of gold porphyrin nanoparticles into normal animals. Finally, tumor size measurement and animal survival were studied to investigate the therapeutic effect of lipidic gold porphyrin nanoparticles on neuroblastoma growth.Results: We found that incorporation of gold porphyrin into lipidic nanoparticles resulted in a 16-fold increase in size. Subsequent in vitro and in vivo cytotoxicity studies further showed that the lipidic gold porphyrin nanoparticles could decrease systemic toxicity, as well as inhibiting tumor growth following administration into the neuroblastoma bearing mice.Conclusion: The delivery of lipidic gold porphyrin nanoparticles by incorporation with lipidic formulation is feasible approach to treat neuroblastoma. We await further studies to evaluate tumor killing kinetics.Keywords: nanoparticles, lipidic formulation, gold porphyrin, neuroblastoma Puiyan LeeYifan ZhuJessie J Yanet alDove Medical PressarticleMedical technologyR855-855.5Chemical technologyTP1-1185ENNanotechnology, Science and Applications, Vol 2010, Iss default, Pp 23-28 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medical technology R855-855.5 Chemical technology TP1-1185
spellingShingle	Medical technology R855-855.5 Chemical technology TP1-1185 Puiyan Lee Yifan Zhu Jessie J Yan et al The cytotoxic effects of lipidic formulated gold porphyrin nanoparticles for the treatment of neuroblastoma
description	Puiyan Lee1, Yifan Zhu1, Jessie J Yan2, Raymond WY Sun2, Wei Hao1, Xuelai Liu1, Chi-Ming Che2, Kenneth KY Wong11Department of Surgery, Li Ka Shing Faculty of Medicine, 2Department of Chemistry and Open Laboratory of Chemical Biology of the Institute of Molecular Technology for Drug Discovery and Synthesis, The University of Hong Kong, Hong Kong Objective: Nanotechnology has been identified as a promising platform in the improvement of the design and development of drug delivery systems. In the present study we investigated the potential of lipidic nanoparticles consisting of gold porphyrin for the treatment of neuroblastoma.Materials and methods: To characterize the size of the lipidic gold porphyrin nanoparticles, we used transmission electron microscopy (TEM). The in vitro cytotoxic effect on neuroblastoma activity was examined using XTT cell proliferation assay, then IC50 values were calculated. In vivo safety and toxicity were studied using intraperitoneal injection of gold porphyrin nanoparticles into normal animals. Finally, tumor size measurement and animal survival were studied to investigate the therapeutic effect of lipidic gold porphyrin nanoparticles on neuroblastoma growth.Results: We found that incorporation of gold porphyrin into lipidic nanoparticles resulted in a 16-fold increase in size. Subsequent in vitro and in vivo cytotoxicity studies further showed that the lipidic gold porphyrin nanoparticles could decrease systemic toxicity, as well as inhibiting tumor growth following administration into the neuroblastoma bearing mice.Conclusion: The delivery of lipidic gold porphyrin nanoparticles by incorporation with lipidic formulation is feasible approach to treat neuroblastoma. We await further studies to evaluate tumor killing kinetics.Keywords: nanoparticles, lipidic formulation, gold porphyrin, neuroblastoma
format	article
author	Puiyan Lee Yifan Zhu Jessie J Yan et al
author_facet	Puiyan Lee Yifan Zhu Jessie J Yan et al
author_sort	Puiyan Lee
title	The cytotoxic effects of lipidic formulated gold porphyrin nanoparticles for the treatment of neuroblastoma
title_short	The cytotoxic effects of lipidic formulated gold porphyrin nanoparticles for the treatment of neuroblastoma
title_full	The cytotoxic effects of lipidic formulated gold porphyrin nanoparticles for the treatment of neuroblastoma
title_fullStr	The cytotoxic effects of lipidic formulated gold porphyrin nanoparticles for the treatment of neuroblastoma
title_full_unstemmed	The cytotoxic effects of lipidic formulated gold porphyrin nanoparticles for the treatment of neuroblastoma
title_sort	cytotoxic effects of lipidic formulated gold porphyrin nanoparticles for the treatment of neuroblastoma
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/8b487cfabb64448d99edc1f440bdf6ed
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The cytotoxic effects of lipidic formulated gold porphyrin nanoparticles for the treatment of neuroblastoma

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