Hydrogen sulfide toxicity inhibits primary root growth through the ROS-NO pathway

Abstract High concentrations of hydrogen sulfide (H2S) are toxic to plants and inhibit their growth. Previous research indicated that high concentrations of H2S modulate the root system architecture (RSA) by affecting auxin transport; however, the signaling pathway underlying this process remains un...

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Autores principales:	Ping Zhang, Qiong Luo, Ruling Wang, Jin Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b4a12cbd14c4e01be2d284f7b0db1da
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spelling	oai:doaj.org-article:8b4a12cbd14c4e01be2d284f7b0db1da2021-12-02T15:06:21ZHydrogen sulfide toxicity inhibits primary root growth through the ROS-NO pathway10.1038/s41598-017-01046-22045-2322https://doaj.org/article/8b4a12cbd14c4e01be2d284f7b0db1da2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01046-2https://doaj.org/toc/2045-2322Abstract High concentrations of hydrogen sulfide (H2S) are toxic to plants and inhibit their growth. Previous research indicated that high concentrations of H2S modulate the root system architecture (RSA) by affecting auxin transport; however, the signaling pathway underlying this process remains unclear. Here, we investigated the effects of exogenous sodium hydrosulfide (NaHS), an H2S donor, on primary root (PR) growth in Arabidopsis using pharmacological, physiological, and genetic approaches. H2S toxicity repressed PR growth by triggering a signal transduction pathway involving reactive oxygen species (ROS) accumulation, MITOGEN-ACTIVATED PROTEIN KINASE 6 (MPK6) activation, and nitric oxide (NO) production. Respiratory burst oxidase homolog mutants and an NO synthase mutant were less sensitive to NaHS, suggesting that both ROS and NO mediate the inhibitory effects of H2S on PR growth. We found that exogenous H2S-activated ROS production was required for NO generation and that MPK6 mediated H2S-induced NO production. MPK6 was shown to function downstream of ROS and upstream of NO. Finally, we demonstrated that exogenous H2S repressed the distribution of auxin and reduced the meristematic cell division potential in root tips, and NO was involved in this process.Ping ZhangQiong LuoRuling WangJin XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ping Zhang Qiong Luo Ruling Wang Jin Xu Hydrogen sulfide toxicity inhibits primary root growth through the ROS-NO pathway
description	Abstract High concentrations of hydrogen sulfide (H2S) are toxic to plants and inhibit their growth. Previous research indicated that high concentrations of H2S modulate the root system architecture (RSA) by affecting auxin transport; however, the signaling pathway underlying this process remains unclear. Here, we investigated the effects of exogenous sodium hydrosulfide (NaHS), an H2S donor, on primary root (PR) growth in Arabidopsis using pharmacological, physiological, and genetic approaches. H2S toxicity repressed PR growth by triggering a signal transduction pathway involving reactive oxygen species (ROS) accumulation, MITOGEN-ACTIVATED PROTEIN KINASE 6 (MPK6) activation, and nitric oxide (NO) production. Respiratory burst oxidase homolog mutants and an NO synthase mutant were less sensitive to NaHS, suggesting that both ROS and NO mediate the inhibitory effects of H2S on PR growth. We found that exogenous H2S-activated ROS production was required for NO generation and that MPK6 mediated H2S-induced NO production. MPK6 was shown to function downstream of ROS and upstream of NO. Finally, we demonstrated that exogenous H2S repressed the distribution of auxin and reduced the meristematic cell division potential in root tips, and NO was involved in this process.
format	article
author	Ping Zhang Qiong Luo Ruling Wang Jin Xu
author_facet	Ping Zhang Qiong Luo Ruling Wang Jin Xu
author_sort	Ping Zhang
title	Hydrogen sulfide toxicity inhibits primary root growth through the ROS-NO pathway
title_short	Hydrogen sulfide toxicity inhibits primary root growth through the ROS-NO pathway
title_full	Hydrogen sulfide toxicity inhibits primary root growth through the ROS-NO pathway
title_fullStr	Hydrogen sulfide toxicity inhibits primary root growth through the ROS-NO pathway
title_full_unstemmed	Hydrogen sulfide toxicity inhibits primary root growth through the ROS-NO pathway
title_sort	hydrogen sulfide toxicity inhibits primary root growth through the ros-no pathway
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8b4a12cbd14c4e01be2d284f7b0db1da
work_keys_str_mv	AT pingzhang hydrogensulfidetoxicityinhibitsprimaryrootgrowththroughtherosnopathway AT qiongluo hydrogensulfidetoxicityinhibitsprimaryrootgrowththroughtherosnopathway AT rulingwang hydrogensulfidetoxicityinhibitsprimaryrootgrowththroughtherosnopathway AT jinxu hydrogensulfidetoxicityinhibitsprimaryrootgrowththroughtherosnopathway
_version_	1718388527316598784

Hydrogen sulfide toxicity inhibits primary root growth through the ROS-NO pathway

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