Circular RNA hsa_circ_0005567 overexpression promotes M2 type macrophage polarization through miR-492/SOCS2 axis to inhibit osteoarthritis progression

Synovial macrophage polarization is essential for osteoarthritis (OA) development. Our study aims to investigate the underlying function and the molecular mechanisms of hsa_circ_0005567 in macrophage polarization. Circular RNA (CircRNA), microRNA (miRNA), and mRNA expression levels were detected by...

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Autores principales:	Jinling Zhang, Fangyue Cheng, Genxiang Rong, Zhi Tang, Binjie Gui
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	osteoarthritis hsa_circ_0005567 macrophage polarization chondrocyte Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/8b52e83ee479450fbb1c05b7e7595a30
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spelling	oai:doaj.org-article:8b52e83ee479450fbb1c05b7e7595a302021-11-04T15:51:53ZCircular RNA hsa_circ_0005567 overexpression promotes M2 type macrophage polarization through miR-492/SOCS2 axis to inhibit osteoarthritis progression2165-59792165-598710.1080/21655979.2021.1989999https://doaj.org/article/8b52e83ee479450fbb1c05b7e7595a302021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1989999https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Synovial macrophage polarization is essential for osteoarthritis (OA) development. Our study aims to investigate the underlying function and the molecular mechanisms of hsa_circ_0005567 in macrophage polarization. Circular RNA (CircRNA), microRNA (miRNA), and mRNA expression levels were detected by quantitative reverse transcription polymerase chain reaction (RT-qPCR). RNA pull down, luciferase reporter were employed to test the interaction between miR-492 and hsa_circ_0005567/suppressors of cytokine signaling 2 (SOCS2). Ectopic overexpression was used to evaluate the function of hsa_circ_0005567. The supernatant of THP-1 cells was used to incubate chondrocytes. Cell Counting Kit-8 (CCK-8) and flow cytometry were conducted to determine cell viability, proportion of M1 or M2 macrophages and apoptotic rate. The results showed that the hsa_circ_0005567 expression level was downregulated in the synovial tissues of osteoarthritis patients. Overexpression of hsa_circ_0005567 inhibited M1 macrophage polarization, and promoted M2 macrophage polarization. Hsa_circ_0005567 was proved to be a molecular sponge for miR-492, and SOCS2 was verified as the target of miR-492. MiR-492 mimic could reverse the effect of hsa_circ_0005567 overexpression on macrophage polarization. Besides, the supernatant from LPS-treated THP-1 macrophage significantly decreased chondrocytes cell viability and increased cell apoptosis ratio, which was reversed by hsa_circ_0005567 overexpression. In conclusion, hsa_circ_0005567 overexpression promoted M2 macrophage polarization through miR-492/SOCS2 axis to reduced chondrocyte apoptosis, which could inhibit osteoarthritis progression.Jinling ZhangFangyue ChengGenxiang RongZhi TangBinjie GuiTaylor & Francis Grouparticleosteoarthritishsa_circ_0005567macrophage polarizationchondrocyteBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 8920-8930 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	osteoarthritis hsa_circ_0005567 macrophage polarization chondrocyte Biotechnology TP248.13-248.65
spellingShingle	osteoarthritis hsa_circ_0005567 macrophage polarization chondrocyte Biotechnology TP248.13-248.65 Jinling Zhang Fangyue Cheng Genxiang Rong Zhi Tang Binjie Gui Circular RNA hsa_circ_0005567 overexpression promotes M2 type macrophage polarization through miR-492/SOCS2 axis to inhibit osteoarthritis progression
description	Synovial macrophage polarization is essential for osteoarthritis (OA) development. Our study aims to investigate the underlying function and the molecular mechanisms of hsa_circ_0005567 in macrophage polarization. Circular RNA (CircRNA), microRNA (miRNA), and mRNA expression levels were detected by quantitative reverse transcription polymerase chain reaction (RT-qPCR). RNA pull down, luciferase reporter were employed to test the interaction between miR-492 and hsa_circ_0005567/suppressors of cytokine signaling 2 (SOCS2). Ectopic overexpression was used to evaluate the function of hsa_circ_0005567. The supernatant of THP-1 cells was used to incubate chondrocytes. Cell Counting Kit-8 (CCK-8) and flow cytometry were conducted to determine cell viability, proportion of M1 or M2 macrophages and apoptotic rate. The results showed that the hsa_circ_0005567 expression level was downregulated in the synovial tissues of osteoarthritis patients. Overexpression of hsa_circ_0005567 inhibited M1 macrophage polarization, and promoted M2 macrophage polarization. Hsa_circ_0005567 was proved to be a molecular sponge for miR-492, and SOCS2 was verified as the target of miR-492. MiR-492 mimic could reverse the effect of hsa_circ_0005567 overexpression on macrophage polarization. Besides, the supernatant from LPS-treated THP-1 macrophage significantly decreased chondrocytes cell viability and increased cell apoptosis ratio, which was reversed by hsa_circ_0005567 overexpression. In conclusion, hsa_circ_0005567 overexpression promoted M2 macrophage polarization through miR-492/SOCS2 axis to reduced chondrocyte apoptosis, which could inhibit osteoarthritis progression.
format	article
author	Jinling Zhang Fangyue Cheng Genxiang Rong Zhi Tang Binjie Gui
author_facet	Jinling Zhang Fangyue Cheng Genxiang Rong Zhi Tang Binjie Gui
author_sort	Jinling Zhang
title	Circular RNA hsa_circ_0005567 overexpression promotes M2 type macrophage polarization through miR-492/SOCS2 axis to inhibit osteoarthritis progression
title_short	Circular RNA hsa_circ_0005567 overexpression promotes M2 type macrophage polarization through miR-492/SOCS2 axis to inhibit osteoarthritis progression
title_full	Circular RNA hsa_circ_0005567 overexpression promotes M2 type macrophage polarization through miR-492/SOCS2 axis to inhibit osteoarthritis progression
title_fullStr	Circular RNA hsa_circ_0005567 overexpression promotes M2 type macrophage polarization through miR-492/SOCS2 axis to inhibit osteoarthritis progression
title_full_unstemmed	Circular RNA hsa_circ_0005567 overexpression promotes M2 type macrophage polarization through miR-492/SOCS2 axis to inhibit osteoarthritis progression
title_sort	circular rna hsa_circ_0005567 overexpression promotes m2 type macrophage polarization through mir-492/socs2 axis to inhibit osteoarthritis progression
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/8b52e83ee479450fbb1c05b7e7595a30
work_keys_str_mv	AT jinlingzhang circularrnahsacirc0005567overexpressionpromotesm2typemacrophagepolarizationthroughmir492socs2axistoinhibitosteoarthritisprogression AT fangyuecheng circularrnahsacirc0005567overexpressionpromotesm2typemacrophagepolarizationthroughmir492socs2axistoinhibitosteoarthritisprogression AT genxiangrong circularrnahsacirc0005567overexpressionpromotesm2typemacrophagepolarizationthroughmir492socs2axistoinhibitosteoarthritisprogression AT zhitang circularrnahsacirc0005567overexpressionpromotesm2typemacrophagepolarizationthroughmir492socs2axistoinhibitosteoarthritisprogression AT binjiegui circularrnahsacirc0005567overexpressionpromotesm2typemacrophagepolarizationthroughmir492socs2axistoinhibitosteoarthritisprogression
_version_	1718444726313549824

Circular RNA hsa_circ_0005567 overexpression promotes M2 type macrophage polarization through miR-492/SOCS2 axis to inhibit osteoarthritis progression

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