Hedyotis corymbosa L. and Sterculia quadrifida R.Br Ethanolic Extract Enhances Cisplatin’s Cytotoxicity on T47D Breast Cancer Cells Through Cell Cycle Modulation

Chemotherapeutic agent such as cisplatin used as cancer cure with side effects and resistance. Ursolic acid is a compound found in Hedyotis corymbosa L., (HCoL) while 8-hydroxydehydrodunnione is found in Sterculia quadrifida R.Br (SQuR) which are proven to have cytotoxic effect to cancer cells. This...

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Autor principal:	Rollando Rollando
Formato:	article
Lenguaje:	EN
Publicado:	University of Brawijaya 2018
Materias:	cisplatin cytoxicity hedyotis corymbosa l sterculia quadrifida r.br. Chemistry QD1-999
Acceso en línea:	http://dx.doi.org/10.21776/ub.jpacr.2018.007.02.400 https://doaj.org/article/8b540554974d44e685997e170d330610
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spelling	oai:doaj.org-article:8b540554974d44e685997e170d3306102021-12-02T13:33:21ZHedyotis corymbosa L. and Sterculia quadrifida R.Br Ethanolic Extract Enhances Cisplatin’s Cytotoxicity on T47D Breast Cancer Cells Through Cell Cycle Modulationhttp://dx.doi.org/10.21776/ub.jpacr.2018.007.02.4002302-46902302-4690https://doaj.org/article/8b540554974d44e685997e170d3306102018-05-01T00:00:00Zhttps://jpacr.ub.ac.id/index.php/jpacr/article/view/400/pdfhttps://doaj.org/toc/2302-4690https://doaj.org/toc/2302-4690Chemotherapeutic agent such as cisplatin used as cancer cure with side effects and resistance. Ursolic acid is a compound found in Hedyotis corymbosa L., (HCoL) while 8-hydroxydehydrodunnione is found in Sterculia quadrifida R.Br (SQuR) which are proven to have cytotoxic effect to cancer cells. This research aims to review the effect of cisplatin, ethanolic extract of HCoL and SQuR to sensitivity increase on breast cancer cells, which will be confirmed through apoptosis induction and cell cycle modulation. The cytotoxic effect was tested using MTT assay on T47D cell by using IC50 parameter. The combination was tested by determining their combination index (CI) and cell viability. The combination effect in apoptosis induction and cell cycle modulation were observed using flow cytometry method. The cytotoxic test result of the combination shows CI value of below 1 at the concentration of HCoL ethanolic extract as much as 0,21 µg/mL, SQuR ethanolic extract as much as 0,45 µg/mL, and cisplatin as much as 2,5 µM. The combination of HCoL ethanolic extract, SQuR ethanolic extract, and cisplatin results in phase S cell accumulation (27,73%) on breast cancer cell T47D and was able to induct apoptosis. The result proves that ethanolic extract of HCoL and SQuR can developed as a co-chemotherapeutic agent to increase the effectivity of breast cancer treatment.Rollando RollandoUniversity of Brawijayaarticlecisplatincytoxicityhedyotis corymbosa lsterculia quadrifida r.br.ChemistryQD1-999ENJournal of Pure and Applied Chemistry Research, Vol 7, Iss 2, Pp 160-172 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	cisplatin cytoxicity hedyotis corymbosa l sterculia quadrifida r.br. Chemistry QD1-999
spellingShingle	cisplatin cytoxicity hedyotis corymbosa l sterculia quadrifida r.br. Chemistry QD1-999 Rollando Rollando Hedyotis corymbosa L. and Sterculia quadrifida R.Br Ethanolic Extract Enhances Cisplatin’s Cytotoxicity on T47D Breast Cancer Cells Through Cell Cycle Modulation
description	Chemotherapeutic agent such as cisplatin used as cancer cure with side effects and resistance. Ursolic acid is a compound found in Hedyotis corymbosa L., (HCoL) while 8-hydroxydehydrodunnione is found in Sterculia quadrifida R.Br (SQuR) which are proven to have cytotoxic effect to cancer cells. This research aims to review the effect of cisplatin, ethanolic extract of HCoL and SQuR to sensitivity increase on breast cancer cells, which will be confirmed through apoptosis induction and cell cycle modulation. The cytotoxic effect was tested using MTT assay on T47D cell by using IC50 parameter. The combination was tested by determining their combination index (CI) and cell viability. The combination effect in apoptosis induction and cell cycle modulation were observed using flow cytometry method. The cytotoxic test result of the combination shows CI value of below 1 at the concentration of HCoL ethanolic extract as much as 0,21 µg/mL, SQuR ethanolic extract as much as 0,45 µg/mL, and cisplatin as much as 2,5 µM. The combination of HCoL ethanolic extract, SQuR ethanolic extract, and cisplatin results in phase S cell accumulation (27,73%) on breast cancer cell T47D and was able to induct apoptosis. The result proves that ethanolic extract of HCoL and SQuR can developed as a co-chemotherapeutic agent to increase the effectivity of breast cancer treatment.
format	article
author	Rollando Rollando
author_facet	Rollando Rollando
author_sort	Rollando Rollando
title	Hedyotis corymbosa L. and Sterculia quadrifida R.Br Ethanolic Extract Enhances Cisplatin’s Cytotoxicity on T47D Breast Cancer Cells Through Cell Cycle Modulation
title_short	Hedyotis corymbosa L. and Sterculia quadrifida R.Br Ethanolic Extract Enhances Cisplatin’s Cytotoxicity on T47D Breast Cancer Cells Through Cell Cycle Modulation
title_full	Hedyotis corymbosa L. and Sterculia quadrifida R.Br Ethanolic Extract Enhances Cisplatin’s Cytotoxicity on T47D Breast Cancer Cells Through Cell Cycle Modulation
title_fullStr	Hedyotis corymbosa L. and Sterculia quadrifida R.Br Ethanolic Extract Enhances Cisplatin’s Cytotoxicity on T47D Breast Cancer Cells Through Cell Cycle Modulation
title_full_unstemmed	Hedyotis corymbosa L. and Sterculia quadrifida R.Br Ethanolic Extract Enhances Cisplatin’s Cytotoxicity on T47D Breast Cancer Cells Through Cell Cycle Modulation
title_sort	hedyotis corymbosa l. and sterculia quadrifida r.br ethanolic extract enhances cisplatin’s cytotoxicity on t47d breast cancer cells through cell cycle modulation
publisher	University of Brawijaya
publishDate	2018
url	http://dx.doi.org/10.21776/ub.jpacr.2018.007.02.400 https://doaj.org/article/8b540554974d44e685997e170d330610
work_keys_str_mv	AT rollandorollando hedyotiscorymbosalandsterculiaquadrifidarbrethanolicextractenhancescisplatinscytotoxicityont47dbreastcancercellsthroughcellcyclemodulation
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Hedyotis corymbosa L. and Sterculia quadrifida R.Br Ethanolic Extract Enhances Cisplatin’s Cytotoxicity on T47D Breast Cancer Cells Through Cell Cycle Modulation

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