Universal extracellular vesicles and PD-L1+ extracellular vesicles detected by single molecule array technology as circulating biomarkers for diffuse large B cell lymphoma
Plasma extracellular vesicles (EVs) have been reported to be a promising source of diagnostic and prognostic biomarkers in various cancers. However, further research in this area is needed due to the limitations of circulating extracellular vesicles detection methods. Using the Single Molecule array...
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Taylor & Francis Group
2021
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oai:doaj.org-article:8b558ba7f82c4d53970fe304f5188d8c2021-11-04T15:00:45ZUniversal extracellular vesicles and PD-L1+ extracellular vesicles detected by single molecule array technology as circulating biomarkers for diffuse large B cell lymphoma2162-402X10.1080/2162402X.2021.1995166https://doaj.org/article/8b558ba7f82c4d53970fe304f5188d8c2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/2162402X.2021.1995166https://doaj.org/toc/2162-402XPlasma extracellular vesicles (EVs) have been reported to be a promising source of diagnostic and prognostic biomarkers in various cancers. However, further research in this area is needed due to the limitations of circulating extracellular vesicles detection methods. Using the Single Molecule array (SiMoa) technology, we developed two extracellular vesicle detection assays, CD9-CD63 and PD-L1-CD63, to determine circulating universal EVs and PD-L1 positive EVs, respectively. A total of 164 diffuse large B-cell lymphoma (DLBCL) patients were retrospectively included in this study. Compared with healthy volunteers (n = 25), elevated CD9-CD63 and PD-L1-CD63 signals were detected in the plasma of DLBCL patients (n = 164). High CD9-CD63 signals was associated with molecular subtype, extranodal site and treatment response in DLBCL. A high PD-L1-CD63 signal was also associated with certain clinical features, including extranodal site and treatment response. CD9-CD63 and PD-L1-CD63 signals were found to be important prognostic factors for both progression-free and overall survival. Furthermore, PD-L1-positive EVs were found in all patients, though PD-L1 protein expression was positive in only 35.4% (17/48) of tumor biopsies. No correlation was found between circulating PD-L1+ EVs and soluble PD-L1 (sPD-L1) levels. Our results show that plasma universal EV and PD-L1-positive EV levels are significantly elevated in DLBCL and might serve as biomarkers for predicting survival outcomes in DLBCL patients.Ji-Wei LiDi ShiXiao-Chun WanJue HuYi-Fan SuYu-Peng ZengZi-Juan HuBao-Hua YuQun-Ling ZhangPing WeiXiao-Yan ZhouTaylor & Francis Grouparticledlbcllymphomaplasmaextracellular vesiclespd-l1Immunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENOncoImmunology, Vol 10, Iss 1 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
dlbcl lymphoma plasma extracellular vesicles pd-l1 Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 |
| spellingShingle |
dlbcl lymphoma plasma extracellular vesicles pd-l1 Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Ji-Wei Li Di Shi Xiao-Chun Wan Jue Hu Yi-Fan Su Yu-Peng Zeng Zi-Juan Hu Bao-Hua Yu Qun-Ling Zhang Ping Wei Xiao-Yan Zhou Universal extracellular vesicles and PD-L1+ extracellular vesicles detected by single molecule array technology as circulating biomarkers for diffuse large B cell lymphoma |
| description |
Plasma extracellular vesicles (EVs) have been reported to be a promising source of diagnostic and prognostic biomarkers in various cancers. However, further research in this area is needed due to the limitations of circulating extracellular vesicles detection methods. Using the Single Molecule array (SiMoa) technology, we developed two extracellular vesicle detection assays, CD9-CD63 and PD-L1-CD63, to determine circulating universal EVs and PD-L1 positive EVs, respectively. A total of 164 diffuse large B-cell lymphoma (DLBCL) patients were retrospectively included in this study. Compared with healthy volunteers (n = 25), elevated CD9-CD63 and PD-L1-CD63 signals were detected in the plasma of DLBCL patients (n = 164). High CD9-CD63 signals was associated with molecular subtype, extranodal site and treatment response in DLBCL. A high PD-L1-CD63 signal was also associated with certain clinical features, including extranodal site and treatment response. CD9-CD63 and PD-L1-CD63 signals were found to be important prognostic factors for both progression-free and overall survival. Furthermore, PD-L1-positive EVs were found in all patients, though PD-L1 protein expression was positive in only 35.4% (17/48) of tumor biopsies. No correlation was found between circulating PD-L1+ EVs and soluble PD-L1 (sPD-L1) levels. Our results show that plasma universal EV and PD-L1-positive EV levels are significantly elevated in DLBCL and might serve as biomarkers for predicting survival outcomes in DLBCL patients. |
| format |
article |
| author |
Ji-Wei Li Di Shi Xiao-Chun Wan Jue Hu Yi-Fan Su Yu-Peng Zeng Zi-Juan Hu Bao-Hua Yu Qun-Ling Zhang Ping Wei Xiao-Yan Zhou |
| author_facet |
Ji-Wei Li Di Shi Xiao-Chun Wan Jue Hu Yi-Fan Su Yu-Peng Zeng Zi-Juan Hu Bao-Hua Yu Qun-Ling Zhang Ping Wei Xiao-Yan Zhou |
| author_sort |
Ji-Wei Li |
| title |
Universal extracellular vesicles and PD-L1+ extracellular vesicles detected by single molecule array technology as circulating biomarkers for diffuse large B cell lymphoma |
| title_short |
Universal extracellular vesicles and PD-L1+ extracellular vesicles detected by single molecule array technology as circulating biomarkers for diffuse large B cell lymphoma |
| title_full |
Universal extracellular vesicles and PD-L1+ extracellular vesicles detected by single molecule array technology as circulating biomarkers for diffuse large B cell lymphoma |
| title_fullStr |
Universal extracellular vesicles and PD-L1+ extracellular vesicles detected by single molecule array technology as circulating biomarkers for diffuse large B cell lymphoma |
| title_full_unstemmed |
Universal extracellular vesicles and PD-L1+ extracellular vesicles detected by single molecule array technology as circulating biomarkers for diffuse large B cell lymphoma |
| title_sort |
universal extracellular vesicles and pd-l1+ extracellular vesicles detected by single molecule array technology as circulating biomarkers for diffuse large b cell lymphoma |
| publisher |
Taylor & Francis Group |
| publishDate |
2021 |
| url |
https://doaj.org/article/8b558ba7f82c4d53970fe304f5188d8c |
| work_keys_str_mv |
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