Forward genetic screening for the improved production of fermentable sugars from plant biomass.

With their unique metabolism and the potential to produce large amounts of biomass, plants are an excellent bio-energy feedstock for a variety of industrial purposes. Here we developed a high-throughput strategy, using the model plant Arabidopsis thaliana, to identify mutants with improved sugar rel...

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Autores principales:	George Stamatiou, Danielle P Vidaurre, Isaac Shim, Xurong Tang, Wolfgang Moeder, Dario Bonetta, Peter McCourt
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b561e42957e4cbe98f63f56cda62dee
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spelling	oai:doaj.org-article:8b561e42957e4cbe98f63f56cda62dee2021-11-18T07:59:06ZForward genetic screening for the improved production of fermentable sugars from plant biomass.1932-620310.1371/journal.pone.0055616https://doaj.org/article/8b561e42957e4cbe98f63f56cda62dee2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23383246/?tool=EBIhttps://doaj.org/toc/1932-6203With their unique metabolism and the potential to produce large amounts of biomass, plants are an excellent bio-energy feedstock for a variety of industrial purposes. Here we developed a high-throughput strategy, using the model plant Arabidopsis thaliana, to identify mutants with improved sugar release from plant biomass. Molecular analysis indicates a variety of processes including starch degradation, cell wall composition and polar transport of the plant hormone auxin can contribute to this improved saccharification. To demonstrate translatability, polar auxin transport in maize was either genetically or chemical inhibited and this also resulted in increased sugar release from plant tissues. Our forward genetic approach using Arabidopsis not only uncovers new functions that contribute to cell wall integrity but also demonstrates that information gleaned from this genetic model can be directly translated to monocotyledonous crops such as maize to improve sugar extractability from biomass.George StamatiouDanielle P VidaurreIsaac ShimXurong TangWolfgang MoederDario BonettaPeter McCourtPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e55616 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q George Stamatiou Danielle P Vidaurre Isaac Shim Xurong Tang Wolfgang Moeder Dario Bonetta Peter McCourt Forward genetic screening for the improved production of fermentable sugars from plant biomass.
description	With their unique metabolism and the potential to produce large amounts of biomass, plants are an excellent bio-energy feedstock for a variety of industrial purposes. Here we developed a high-throughput strategy, using the model plant Arabidopsis thaliana, to identify mutants with improved sugar release from plant biomass. Molecular analysis indicates a variety of processes including starch degradation, cell wall composition and polar transport of the plant hormone auxin can contribute to this improved saccharification. To demonstrate translatability, polar auxin transport in maize was either genetically or chemical inhibited and this also resulted in increased sugar release from plant tissues. Our forward genetic approach using Arabidopsis not only uncovers new functions that contribute to cell wall integrity but also demonstrates that information gleaned from this genetic model can be directly translated to monocotyledonous crops such as maize to improve sugar extractability from biomass.
format	article
author	George Stamatiou Danielle P Vidaurre Isaac Shim Xurong Tang Wolfgang Moeder Dario Bonetta Peter McCourt
author_facet	George Stamatiou Danielle P Vidaurre Isaac Shim Xurong Tang Wolfgang Moeder Dario Bonetta Peter McCourt
author_sort	George Stamatiou
title	Forward genetic screening for the improved production of fermentable sugars from plant biomass.
title_short	Forward genetic screening for the improved production of fermentable sugars from plant biomass.
title_full	Forward genetic screening for the improved production of fermentable sugars from plant biomass.
title_fullStr	Forward genetic screening for the improved production of fermentable sugars from plant biomass.
title_full_unstemmed	Forward genetic screening for the improved production of fermentable sugars from plant biomass.
title_sort	forward genetic screening for the improved production of fermentable sugars from plant biomass.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/8b561e42957e4cbe98f63f56cda62dee
work_keys_str_mv	AT georgestamatiou forwardgeneticscreeningfortheimprovedproductionoffermentablesugarsfromplantbiomass AT daniellepvidaurre forwardgeneticscreeningfortheimprovedproductionoffermentablesugarsfromplantbiomass AT isaacshim forwardgeneticscreeningfortheimprovedproductionoffermentablesugarsfromplantbiomass AT xurongtang forwardgeneticscreeningfortheimprovedproductionoffermentablesugarsfromplantbiomass AT wolfgangmoeder forwardgeneticscreeningfortheimprovedproductionoffermentablesugarsfromplantbiomass AT dariobonetta forwardgeneticscreeningfortheimprovedproductionoffermentablesugarsfromplantbiomass AT petermccourt forwardgeneticscreeningfortheimprovedproductionoffermentablesugarsfromplantbiomass
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Forward genetic screening for the improved production of fermentable sugars from plant biomass.

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