A Novel Multi-Ingredient Supplement Activates a Browning Program in White Adipose Tissue and Mitigates Weight Gain in High-Fat Diet-Fed Mice
We investigated the effects of a novel multi-ingredient supplement comprised of polyphenol antioxidants and compounds known to facilitate mitochondrial function and metabolic enhancement (ME) in a mouse model of obesity. In this study, 6-week-old male C57/BL6J mice were placed on a high-fat diet (HF...
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MDPI AG
2021
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oai:doaj.org-article:8b56b4bee0b74a83a19a7e3c30898ae52021-11-25T18:33:32ZA Novel Multi-Ingredient Supplement Activates a Browning Program in White Adipose Tissue and Mitigates Weight Gain in High-Fat Diet-Fed Mice10.3390/nu131137262072-6643https://doaj.org/article/8b56b4bee0b74a83a19a7e3c30898ae52021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6643/13/11/3726https://doaj.org/toc/2072-6643We investigated the effects of a novel multi-ingredient supplement comprised of polyphenol antioxidants and compounds known to facilitate mitochondrial function and metabolic enhancement (ME) in a mouse model of obesity. In this study, 6-week-old male C57/BL6J mice were placed on a high-fat diet (HFD; ~60% fat) for 6 weeks, with subsequent allocation into experimentalgroups for 4 weeks: HFD control, HFD + ME10 (10 components), HFD + ME7 (7 components), HFD + ME10 + EX, HFD + EX (where ‘+EX’ animals exercised 3 days/week), and chow-fed control. After the intervention, HFD control animals had significantly greater body weight and fat mass. Despite the continuation of HFD, animals supplemented with multi-ingredient ME or who performed exercise training showed an attenuation of fat mass and preservation of lean body mass, which was further enhanced when combined (ME+EX). ME supplementation stimulated the upregulation of white and brown adipose tissue mRNA transcripts associated with mitochondrial biogenesis, browning, fatty acid transport, and fat metabolism. In WAT depots, this was mirrored by mitochodrial oxidative phosphorylation (OXPHOS) protein expression, and increased in vivo fat oxidation measured via CLAMS. ME supplementation also decreased systemic and local inflammation markers. Herein, we demonstrated that novel multi-ingredient nutritional supplements induced significant fat loss independent of physical activity while preserving muscle mass in obese mice. Mechanistically, these MEs appear to act by inducing a browning program in white adipose tissue and decreasing other pathophysiological impairments associated with obesity, including mitochondrial respiration alterations induced by HFD.Joshua P. NederveenKatherine MantaAdam L. BujakAlexander C. SimoneMatthew R. FudaMats I. NilssonBart P. HettingaMeghan C. HughesChristopher G. R. PerryMark A. TarnopolskyMDPI AGarticlemitochondriaobesitymulti-ingredient supplementbrowninghigh-fat dietantioxidantNutrition. Foods and food supplyTX341-641ENNutrients, Vol 13, Iss 3726, p 3726 (2021) |
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mitochondria obesity multi-ingredient supplement browning high-fat diet antioxidant Nutrition. Foods and food supply TX341-641 |
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mitochondria obesity multi-ingredient supplement browning high-fat diet antioxidant Nutrition. Foods and food supply TX341-641 Joshua P. Nederveen Katherine Manta Adam L. Bujak Alexander C. Simone Matthew R. Fuda Mats I. Nilsson Bart P. Hettinga Meghan C. Hughes Christopher G. R. Perry Mark A. Tarnopolsky A Novel Multi-Ingredient Supplement Activates a Browning Program in White Adipose Tissue and Mitigates Weight Gain in High-Fat Diet-Fed Mice |
| description |
We investigated the effects of a novel multi-ingredient supplement comprised of polyphenol antioxidants and compounds known to facilitate mitochondrial function and metabolic enhancement (ME) in a mouse model of obesity. In this study, 6-week-old male C57/BL6J mice were placed on a high-fat diet (HFD; ~60% fat) for 6 weeks, with subsequent allocation into experimentalgroups for 4 weeks: HFD control, HFD + ME10 (10 components), HFD + ME7 (7 components), HFD + ME10 + EX, HFD + EX (where ‘+EX’ animals exercised 3 days/week), and chow-fed control. After the intervention, HFD control animals had significantly greater body weight and fat mass. Despite the continuation of HFD, animals supplemented with multi-ingredient ME or who performed exercise training showed an attenuation of fat mass and preservation of lean body mass, which was further enhanced when combined (ME+EX). ME supplementation stimulated the upregulation of white and brown adipose tissue mRNA transcripts associated with mitochondrial biogenesis, browning, fatty acid transport, and fat metabolism. In WAT depots, this was mirrored by mitochodrial oxidative phosphorylation (OXPHOS) protein expression, and increased in vivo fat oxidation measured via CLAMS. ME supplementation also decreased systemic and local inflammation markers. Herein, we demonstrated that novel multi-ingredient nutritional supplements induced significant fat loss independent of physical activity while preserving muscle mass in obese mice. Mechanistically, these MEs appear to act by inducing a browning program in white adipose tissue and decreasing other pathophysiological impairments associated with obesity, including mitochondrial respiration alterations induced by HFD. |
| format |
article |
| author |
Joshua P. Nederveen Katherine Manta Adam L. Bujak Alexander C. Simone Matthew R. Fuda Mats I. Nilsson Bart P. Hettinga Meghan C. Hughes Christopher G. R. Perry Mark A. Tarnopolsky |
| author_facet |
Joshua P. Nederveen Katherine Manta Adam L. Bujak Alexander C. Simone Matthew R. Fuda Mats I. Nilsson Bart P. Hettinga Meghan C. Hughes Christopher G. R. Perry Mark A. Tarnopolsky |
| author_sort |
Joshua P. Nederveen |
| title |
A Novel Multi-Ingredient Supplement Activates a Browning Program in White Adipose Tissue and Mitigates Weight Gain in High-Fat Diet-Fed Mice |
| title_short |
A Novel Multi-Ingredient Supplement Activates a Browning Program in White Adipose Tissue and Mitigates Weight Gain in High-Fat Diet-Fed Mice |
| title_full |
A Novel Multi-Ingredient Supplement Activates a Browning Program in White Adipose Tissue and Mitigates Weight Gain in High-Fat Diet-Fed Mice |
| title_fullStr |
A Novel Multi-Ingredient Supplement Activates a Browning Program in White Adipose Tissue and Mitigates Weight Gain in High-Fat Diet-Fed Mice |
| title_full_unstemmed |
A Novel Multi-Ingredient Supplement Activates a Browning Program in White Adipose Tissue and Mitigates Weight Gain in High-Fat Diet-Fed Mice |
| title_sort |
novel multi-ingredient supplement activates a browning program in white adipose tissue and mitigates weight gain in high-fat diet-fed mice |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/8b56b4bee0b74a83a19a7e3c30898ae5 |
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