Short Communication: Polymorphism of myostatin gene and its association with body weight traits in a hybrid of GAMA chicken (Gallus gallus domesticus Linn. 1758)

Abstract. Tanjung A, Saragih HTSSG, Trijoko, Soenarwan HP, Widianto S, Mahardhika IWS, Daryono BS. 2019. Polymorphism of myostatin gene and its association with body weight traits in a hybrid of GAMA chicken (Gallus gallus domesticus Linn. 1758). Biodiversitas 20: 3207-3212. An experiment was conduc...

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Autores principales:	ARDO TANJUNG, H.T.S.S.G. SARAGIH, TRIJOKO, H.P. SOENARWAN, S. WIDIANTO, I.W.S. MAHARDHIKA, B.S. DARYONO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	adenine insertion, chicken body weight, genetic marker, myostatin gene, polymorphism Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8b59046144ab4b2f85ab8fa169f04529
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spelling	oai:doaj.org-article:8b59046144ab4b2f85ab8fa169f045292021-11-21T21:57:24ZShort Communication: Polymorphism of myostatin gene and its association with body weight traits in a hybrid of GAMA chicken (Gallus gallus domesticus Linn. 1758)1412-033X2085-472210.13057/biodiv/d201113https://doaj.org/article/8b59046144ab4b2f85ab8fa169f045292019-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4146https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Tanjung A, Saragih HTSSG, Trijoko, Soenarwan HP, Widianto S, Mahardhika IWS, Daryono BS. 2019. Polymorphism of myostatin gene and its association with body weight traits in a hybrid of GAMA chicken (Gallus gallus domesticus Linn. 1758). Biodiversitas 20: 3207-3212. An experiment was conducted to detect SNP of the myostatin gene and its association with the body weight of hybrid chicken crossbreed from F1 Kamper and BC1 Broiler. Four F1 Kamper hens were crossbred with BC1 Broiler rooster. Day old chick (DOC) hatched were maintained for 49 days with body weight measurement every seven days. The blood samples from 49 days old chicken were taken for DNA isolation by Chelex 5% method and then amplification of the myostatin gene. PCR products were sequenced, and sequence alignment was performed using Clustal Omega to obtain SNP. The SNP obtained was analyzed by the Pearson correlation test with the body weight of forty nine-days-old chickens. The body weight of the hybrid chicken is higher than of Pelung chicken but lower than the Broiler. There are 7 SNPs in myostatin gene exons included 2 Adenine insertions, 1 Guanine deletion, and four substitutions (C2244G, G2283A, T4842G, G7378T) that yield nine haplotypes. Six haplotypes had different protein sequences with Myostatin protein, while three haplotypes were identical to Myostatin protein. The correlation analysis showed that there was a strong positive correlation (r = 0.736) between normal Myostatin protein and mutant to chicken body weight at 49-days-old. Adenine insertion to nucleotide 2099-2100 of myostatin gene had a very strong positive correlation (r = 0.800) to 49-days-old chicken body weight, although T4842G substitution had a strong negative relationship (r = -0.773) to 49-days-old chicken body weight. Adenine insertion to nucleotide 2099-2100 of myostatin gene could be a genetic marker of heavier body weight of the hybrid chicken.ARDO TANJUNGH.T.S.S.G. SARAGIHTRIJOKOH.P. SOENARWANS. WIDIANTOI.W.S. MAHARDHIKAB.S. DARYONOMBI & UNS Soloarticleadenine insertion, chicken body weight, genetic marker, myostatin gene, polymorphismBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	adenine insertion, chicken body weight, genetic marker, myostatin gene, polymorphism Biology (General) QH301-705.5
spellingShingle	adenine insertion, chicken body weight, genetic marker, myostatin gene, polymorphism Biology (General) QH301-705.5 ARDO TANJUNG H.T.S.S.G. SARAGIH TRIJOKO H.P. SOENARWAN S. WIDIANTO I.W.S. MAHARDHIKA B.S. DARYONO Short Communication: Polymorphism of myostatin gene and its association with body weight traits in a hybrid of GAMA chicken (Gallus gallus domesticus Linn. 1758)
description	Abstract. Tanjung A, Saragih HTSSG, Trijoko, Soenarwan HP, Widianto S, Mahardhika IWS, Daryono BS. 2019. Polymorphism of myostatin gene and its association with body weight traits in a hybrid of GAMA chicken (Gallus gallus domesticus Linn. 1758). Biodiversitas 20: 3207-3212. An experiment was conducted to detect SNP of the myostatin gene and its association with the body weight of hybrid chicken crossbreed from F1 Kamper and BC1 Broiler. Four F1 Kamper hens were crossbred with BC1 Broiler rooster. Day old chick (DOC) hatched were maintained for 49 days with body weight measurement every seven days. The blood samples from 49 days old chicken were taken for DNA isolation by Chelex 5% method and then amplification of the myostatin gene. PCR products were sequenced, and sequence alignment was performed using Clustal Omega to obtain SNP. The SNP obtained was analyzed by the Pearson correlation test with the body weight of forty nine-days-old chickens. The body weight of the hybrid chicken is higher than of Pelung chicken but lower than the Broiler. There are 7 SNPs in myostatin gene exons included 2 Adenine insertions, 1 Guanine deletion, and four substitutions (C2244G, G2283A, T4842G, G7378T) that yield nine haplotypes. Six haplotypes had different protein sequences with Myostatin protein, while three haplotypes were identical to Myostatin protein. The correlation analysis showed that there was a strong positive correlation (r = 0.736) between normal Myostatin protein and mutant to chicken body weight at 49-days-old. Adenine insertion to nucleotide 2099-2100 of myostatin gene had a very strong positive correlation (r = 0.800) to 49-days-old chicken body weight, although T4842G substitution had a strong negative relationship (r = -0.773) to 49-days-old chicken body weight. Adenine insertion to nucleotide 2099-2100 of myostatin gene could be a genetic marker of heavier body weight of the hybrid chicken.
format	article
author	ARDO TANJUNG H.T.S.S.G. SARAGIH TRIJOKO H.P. SOENARWAN S. WIDIANTO I.W.S. MAHARDHIKA B.S. DARYONO
author_facet	ARDO TANJUNG H.T.S.S.G. SARAGIH TRIJOKO H.P. SOENARWAN S. WIDIANTO I.W.S. MAHARDHIKA B.S. DARYONO
author_sort	ARDO TANJUNG
title	Short Communication: Polymorphism of myostatin gene and its association with body weight traits in a hybrid of GAMA chicken (Gallus gallus domesticus Linn. 1758)
title_short	Short Communication: Polymorphism of myostatin gene and its association with body weight traits in a hybrid of GAMA chicken (Gallus gallus domesticus Linn. 1758)
title_full	Short Communication: Polymorphism of myostatin gene and its association with body weight traits in a hybrid of GAMA chicken (Gallus gallus domesticus Linn. 1758)
title_fullStr	Short Communication: Polymorphism of myostatin gene and its association with body weight traits in a hybrid of GAMA chicken (Gallus gallus domesticus Linn. 1758)
title_full_unstemmed	Short Communication: Polymorphism of myostatin gene and its association with body weight traits in a hybrid of GAMA chicken (Gallus gallus domesticus Linn. 1758)
title_sort	short communication: polymorphism of myostatin gene and its association with body weight traits in a hybrid of gama chicken (gallus gallus domesticus linn. 1758)
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/8b59046144ab4b2f85ab8fa169f04529
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Short Communication: Polymorphism of myostatin gene and its association with body weight traits in a hybrid of GAMA chicken (Gallus gallus domesticus Linn. 1758)

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