Circular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs

Dysregulated circular RNAs (circRNAs) is involved in the progression of non-small cell lung cancer (NSCLC). However, the role of has_circ_0002360 (circ_0002360) in NSCLC has rarely been reported. In this study, circ_0002360 expression in NSCLC tissues and cell lines was measured with microarray data...

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Autores principales:	Yunting Zhang, Shaolin Zeng, Tao Wang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	non-small cell lung cancer circ_0002360 micrornas matrix metalloproteinase 16 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/8b6047f82a4144b1af50b3293b60313f
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spelling	oai:doaj.org-article:8b6047f82a4144b1af50b3293b60313f2021-11-11T14:23:43ZCircular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs2165-59792165-598710.1080/21655979.2021.1999370https://doaj.org/article/8b6047f82a4144b1af50b3293b60313f2021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1999370https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Dysregulated circular RNAs (circRNAs) is involved in the progression of non-small cell lung cancer (NSCLC). However, the role of has_circ_0002360 (circ_0002360) in NSCLC has rarely been reported. In this study, circ_0002360 expression in NSCLC tissues and cell lines was measured with microarray data and quantitative real-time PCR (qRT-PCR). After gain-of-function and loss-of-function cell models were established, 5-bromo-2-deoxyuridine (BrdU) and transwell assays were conducted to detect NSCLC cell growth, migration and invasion. What’s more, bioinformatic analysis and dual-luciferase reporter assay were adopted to show how circ_0002360, microRNAs (miR-127-5p, miR-145-5p, miR-585-3p, and miR-758-3p) and matrix metalloproteinase 16 (MMP16) 3ʹUTR interact each other. Western blot was executed to probe the regulatory effects of circ_0002360 and these miRNAs on MMP16 protein expression in NSCLC cells. We found that circ_0002360 expression was raised in NSCLC tissues. High circ_0002360 expression predicted short overall survival time of NSCLC patients. Circ_0002360 overexpression promoted NSCLC cell proliferative, migrative, and invasive abilities, and circ_0002360 depletion worked oppositely. MiR-127-5p, miR-145-5p, miR-585-3p, and miR-758-3p were the targets of circ_0002360, and circ_0002360 could regulate MMP16 expression by competitively binding with the above miRNAs. In summary, circ_0002360 serves as a competitive endogenous RNA to raise MMP16 expressions by competitively binding to miR-127-5p, miR-145-5p, miR-585-3p, and miR-758-3p, thereby promoting NSCLC progression.Yunting ZhangShaolin ZengTao WangTaylor & Francis Grouparticlenon-small cell lung cancercirc_0002360micrornasmatrix metalloproteinase 16BiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	non-small cell lung cancer circ_0002360 micrornas matrix metalloproteinase 16 Biotechnology TP248.13-248.65
spellingShingle	non-small cell lung cancer circ_0002360 micrornas matrix metalloproteinase 16 Biotechnology TP248.13-248.65 Yunting Zhang Shaolin Zeng Tao Wang Circular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs
description	Dysregulated circular RNAs (circRNAs) is involved in the progression of non-small cell lung cancer (NSCLC). However, the role of has_circ_0002360 (circ_0002360) in NSCLC has rarely been reported. In this study, circ_0002360 expression in NSCLC tissues and cell lines was measured with microarray data and quantitative real-time PCR (qRT-PCR). After gain-of-function and loss-of-function cell models were established, 5-bromo-2-deoxyuridine (BrdU) and transwell assays were conducted to detect NSCLC cell growth, migration and invasion. What’s more, bioinformatic analysis and dual-luciferase reporter assay were adopted to show how circ_0002360, microRNAs (miR-127-5p, miR-145-5p, miR-585-3p, and miR-758-3p) and matrix metalloproteinase 16 (MMP16) 3ʹUTR interact each other. Western blot was executed to probe the regulatory effects of circ_0002360 and these miRNAs on MMP16 protein expression in NSCLC cells. We found that circ_0002360 expression was raised in NSCLC tissues. High circ_0002360 expression predicted short overall survival time of NSCLC patients. Circ_0002360 overexpression promoted NSCLC cell proliferative, migrative, and invasive abilities, and circ_0002360 depletion worked oppositely. MiR-127-5p, miR-145-5p, miR-585-3p, and miR-758-3p were the targets of circ_0002360, and circ_0002360 could regulate MMP16 expression by competitively binding with the above miRNAs. In summary, circ_0002360 serves as a competitive endogenous RNA to raise MMP16 expressions by competitively binding to miR-127-5p, miR-145-5p, miR-585-3p, and miR-758-3p, thereby promoting NSCLC progression.
format	article
author	Yunting Zhang Shaolin Zeng Tao Wang
author_facet	Yunting Zhang Shaolin Zeng Tao Wang
author_sort	Yunting Zhang
title	Circular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs
title_short	Circular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs
title_full	Circular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs
title_fullStr	Circular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs
title_full_unstemmed	Circular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs
title_sort	circular rna hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorrnas
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/8b6047f82a4144b1af50b3293b60313f
work_keys_str_mv	AT yuntingzhang circularrnahsacirc0002360promotesnonsmallcelllungcancerprogressionthroughupregulatingmatrixmetalloproteinase16andspongingmultiplemicorrnas AT shaolinzeng circularrnahsacirc0002360promotesnonsmallcelllungcancerprogressionthroughupregulatingmatrixmetalloproteinase16andspongingmultiplemicorrnas AT taowang circularrnahsacirc0002360promotesnonsmallcelllungcancerprogressionthroughupregulatingmatrixmetalloproteinase16andspongingmultiplemicorrnas
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Circular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs

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