Longitudinal saliva omics responses to immune perturbation: a case study

Abstract Saliva omics has immense potential for non-invasive diagnostics, including monitoring very young or elderly populations, or individuals in remote locations. In this study, multiple saliva omics from an individual were monitored over three periods (100 timepoints) involving: (1) hourly sampl...

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Autores principales:	George I. Mias, Vikas Vikram Singh, Lavida R. K. Rogers, Shuyue Xue, Minzhang Zheng, Sergii Domanskyi, Masamitsu Kanada, Carlo Piermarocchi, Jin He
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b6200b97e7344768922bedde90265da
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spelling	oai:doaj.org-article:8b6200b97e7344768922bedde90265da2021-12-02T14:01:35ZLongitudinal saliva omics responses to immune perturbation: a case study10.1038/s41598-020-80605-62045-2322https://doaj.org/article/8b6200b97e7344768922bedde90265da2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80605-6https://doaj.org/toc/2045-2322Abstract Saliva omics has immense potential for non-invasive diagnostics, including monitoring very young or elderly populations, or individuals in remote locations. In this study, multiple saliva omics from an individual were monitored over three periods (100 timepoints) involving: (1) hourly sampling over 24 h without intervention, (2) hourly sampling over 24 h including immune system activation using the standard 23-valent pneumococcal polysaccharide vaccine, (3) daily sampling for 33 days profiling the post-vaccination response. At each timepoint total saliva transcriptome and proteome, and small RNA from salivary extracellular vesicles were profiled, including mRNA, miRNA, piRNA and bacterial RNA. The two 24-h periods were used in a paired analysis to remove daily variation and reveal vaccination responses. Over 18,000 omics longitudinal series had statistically significant temporal trends compared to a healthy baseline. Various immune response and regulation pathways were activated following vaccination, including interferon and cytokine signaling, and MHC antigen presentation. Immune response timeframes were concordant with innate and adaptive immunity development, and coincided with vaccination and reported fever. Overall, mRNA results appeared more specific and sensitive (timewise) to vaccination compared to other omics. The results suggest saliva omics can be consistently assessed for non-invasive personalized monitoring and immune response diagnostics.George I. MiasVikas Vikram SinghLavida R. K. RogersShuyue XueMinzhang ZhengSergii DomanskyiMasamitsu KanadaCarlo PiermarocchiJin HeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-20 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q George I. Mias Vikas Vikram Singh Lavida R. K. Rogers Shuyue Xue Minzhang Zheng Sergii Domanskyi Masamitsu Kanada Carlo Piermarocchi Jin He Longitudinal saliva omics responses to immune perturbation: a case study
description	Abstract Saliva omics has immense potential for non-invasive diagnostics, including monitoring very young or elderly populations, or individuals in remote locations. In this study, multiple saliva omics from an individual were monitored over three periods (100 timepoints) involving: (1) hourly sampling over 24 h without intervention, (2) hourly sampling over 24 h including immune system activation using the standard 23-valent pneumococcal polysaccharide vaccine, (3) daily sampling for 33 days profiling the post-vaccination response. At each timepoint total saliva transcriptome and proteome, and small RNA from salivary extracellular vesicles were profiled, including mRNA, miRNA, piRNA and bacterial RNA. The two 24-h periods were used in a paired analysis to remove daily variation and reveal vaccination responses. Over 18,000 omics longitudinal series had statistically significant temporal trends compared to a healthy baseline. Various immune response and regulation pathways were activated following vaccination, including interferon and cytokine signaling, and MHC antigen presentation. Immune response timeframes were concordant with innate and adaptive immunity development, and coincided with vaccination and reported fever. Overall, mRNA results appeared more specific and sensitive (timewise) to vaccination compared to other omics. The results suggest saliva omics can be consistently assessed for non-invasive personalized monitoring and immune response diagnostics.
format	article
author	George I. Mias Vikas Vikram Singh Lavida R. K. Rogers Shuyue Xue Minzhang Zheng Sergii Domanskyi Masamitsu Kanada Carlo Piermarocchi Jin He
author_facet	George I. Mias Vikas Vikram Singh Lavida R. K. Rogers Shuyue Xue Minzhang Zheng Sergii Domanskyi Masamitsu Kanada Carlo Piermarocchi Jin He
author_sort	George I. Mias
title	Longitudinal saliva omics responses to immune perturbation: a case study
title_short	Longitudinal saliva omics responses to immune perturbation: a case study
title_full	Longitudinal saliva omics responses to immune perturbation: a case study
title_fullStr	Longitudinal saliva omics responses to immune perturbation: a case study
title_full_unstemmed	Longitudinal saliva omics responses to immune perturbation: a case study
title_sort	longitudinal saliva omics responses to immune perturbation: a case study
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8b6200b97e7344768922bedde90265da
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Longitudinal saliva omics responses to immune perturbation: a case study

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