Natural transformation of Campylobacter jejuni occurs beyond limits of growth.

Campylobacter jejuni is a human bacterial pathogen. While poultry is considered to be a major source of food borne campylobacteriosis, C. jejuni is frequently found in the external environment, and water is another well-known source of human infections. Natural transformation is considered to be one...

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Autores principales:	Christina S Vegge, Lone Brøndsted, Małgorzata Ligowska-Marzęta, Hanne Ingmer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b6606e5201446108ac8320c0712e05d
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spelling	oai:doaj.org-article:8b6606e5201446108ac8320c0712e05d2021-11-18T08:13:56ZNatural transformation of Campylobacter jejuni occurs beyond limits of growth.1932-620310.1371/journal.pone.0045467https://doaj.org/article/8b6606e5201446108ac8320c0712e05d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23049803/?tool=EBIhttps://doaj.org/toc/1932-6203Campylobacter jejuni is a human bacterial pathogen. While poultry is considered to be a major source of food borne campylobacteriosis, C. jejuni is frequently found in the external environment, and water is another well-known source of human infections. Natural transformation is considered to be one of the main mechanisms for mediating transfer of genetic material and evolution of the organism. Given the diverse habitats of C. jejuni we set out to examine how environmental conditions and physiological processes affect natural transformation of C. jejuni. We show that the efficiency of transformation is correlated to the growth conditions, but more importantly that transformation occurs at growth-restrictive conditions as well as in the late stationary phase; hence revealing that growth per se is not required for C. jejuni to be competent. Yet, natural transformation of C. jejuni is an energy dependent process, that occurs in the absence of transcription but requires an active translational machinery. Moreover, we show the ATP dependent ClpP protease to be important for transformation, which possibly could be associated with reduced protein glycosylation in the ClpP mutant. In contrast, competence of C. jejuni was neither found to be involved in DNA repair following DNA damage nor to provide a growth benefit. Kinetic studies revealed that several transformation events occur per cell cycle indicating that natural transformation of C. jejuni is a highly efficient process. Thus, our findings suggest that horizontal gene transfer by natural transformation takes place in various habitats occupied by C. jejuni.Christina S VeggeLone BrøndstedMałgorzata Ligowska-MarzętaHanne IngmerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e45467 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christina S Vegge Lone Brøndsted Małgorzata Ligowska-Marzęta Hanne Ingmer Natural transformation of Campylobacter jejuni occurs beyond limits of growth.
description	Campylobacter jejuni is a human bacterial pathogen. While poultry is considered to be a major source of food borne campylobacteriosis, C. jejuni is frequently found in the external environment, and water is another well-known source of human infections. Natural transformation is considered to be one of the main mechanisms for mediating transfer of genetic material and evolution of the organism. Given the diverse habitats of C. jejuni we set out to examine how environmental conditions and physiological processes affect natural transformation of C. jejuni. We show that the efficiency of transformation is correlated to the growth conditions, but more importantly that transformation occurs at growth-restrictive conditions as well as in the late stationary phase; hence revealing that growth per se is not required for C. jejuni to be competent. Yet, natural transformation of C. jejuni is an energy dependent process, that occurs in the absence of transcription but requires an active translational machinery. Moreover, we show the ATP dependent ClpP protease to be important for transformation, which possibly could be associated with reduced protein glycosylation in the ClpP mutant. In contrast, competence of C. jejuni was neither found to be involved in DNA repair following DNA damage nor to provide a growth benefit. Kinetic studies revealed that several transformation events occur per cell cycle indicating that natural transformation of C. jejuni is a highly efficient process. Thus, our findings suggest that horizontal gene transfer by natural transformation takes place in various habitats occupied by C. jejuni.
format	article
author	Christina S Vegge Lone Brøndsted Małgorzata Ligowska-Marzęta Hanne Ingmer
author_facet	Christina S Vegge Lone Brøndsted Małgorzata Ligowska-Marzęta Hanne Ingmer
author_sort	Christina S Vegge
title	Natural transformation of Campylobacter jejuni occurs beyond limits of growth.
title_short	Natural transformation of Campylobacter jejuni occurs beyond limits of growth.
title_full	Natural transformation of Campylobacter jejuni occurs beyond limits of growth.
title_fullStr	Natural transformation of Campylobacter jejuni occurs beyond limits of growth.
title_full_unstemmed	Natural transformation of Campylobacter jejuni occurs beyond limits of growth.
title_sort	natural transformation of campylobacter jejuni occurs beyond limits of growth.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/8b6606e5201446108ac8320c0712e05d
work_keys_str_mv	AT christinasvegge naturaltransformationofcampylobacterjejunioccursbeyondlimitsofgrowth AT lonebrøndsted naturaltransformationofcampylobacterjejunioccursbeyondlimitsofgrowth AT małgorzataligowskamarzeta naturaltransformationofcampylobacterjejunioccursbeyondlimitsofgrowth AT hanneingmer naturaltransformationofcampylobacterjejunioccursbeyondlimitsofgrowth
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Natural transformation of Campylobacter jejuni occurs beyond limits of growth.

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