Morphological and proteomic responses of Eruca sativa exposed to silver nanoparticles or silver nitrate.

Silver nanoparticles (AgNPs) are widely used in commercial products, and there are growing concerns about their impact on the environment. Information about the molecular interaction of AgNPs with plants is lacking. To increase our understanding of the mechanisms involved in plant responses to AgNPs...

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Autores principales:	Candida Vannini, Guido Domingo, Elisabetta Onelli, Bhakti Prinsi, Milena Marsoni, Luca Espen, Marcella Bracale
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b74753be7194da8a28d79357d0e8fe1
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spelling	oai:doaj.org-article:8b74753be7194da8a28d79357d0e8fe12021-11-18T07:37:17ZMorphological and proteomic responses of Eruca sativa exposed to silver nanoparticles or silver nitrate.1932-620310.1371/journal.pone.0068752https://doaj.org/article/8b74753be7194da8a28d79357d0e8fe12013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23874747/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Silver nanoparticles (AgNPs) are widely used in commercial products, and there are growing concerns about their impact on the environment. Information about the molecular interaction of AgNPs with plants is lacking. To increase our understanding of the mechanisms involved in plant responses to AgNPs and to differentiate between particle specific and ionic silver effects we determined the morphological and proteomic changes induced in Eruca sativa (commonly called rocket) in response to AgNPs or AgNO3. Seedlings were treated for 5 days with different concentrations of AgNPs or AgNO3. A similar increase in root elongation was observed when seedlings were exposed to 10 mg Ag L(1) of either PVP-AgNPs or AgNO3. At this concentration we performed electron microscopy investigations and 2-dimensional electrophoresis (2DE) proteomic profiling. The low level of overlap of differentially expressed proteins indicates that AgNPs and AgNO3 cause different plant responses. Both Ag treatments cause changes in proteins involved in the redox regulation and in the sulfur metabolism. These responses could play an important role to maintain cellular homeostasis. Only the AgNP exposure cause the alteration of some proteins related to the endoplasmic reticulum and vacuole indicating these two organelles as targets of the AgNPs action. These data add further evidences that the effects of AgNPs are not simply due to the release of Ag ions.Candida VanniniGuido DomingoElisabetta OnelliBhakti PrinsiMilena MarsoniLuca EspenMarcella BracalePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68752 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Candida Vannini Guido Domingo Elisabetta Onelli Bhakti Prinsi Milena Marsoni Luca Espen Marcella Bracale Morphological and proteomic responses of Eruca sativa exposed to silver nanoparticles or silver nitrate.
description	Silver nanoparticles (AgNPs) are widely used in commercial products, and there are growing concerns about their impact on the environment. Information about the molecular interaction of AgNPs with plants is lacking. To increase our understanding of the mechanisms involved in plant responses to AgNPs and to differentiate between particle specific and ionic silver effects we determined the morphological and proteomic changes induced in Eruca sativa (commonly called rocket) in response to AgNPs or AgNO3. Seedlings were treated for 5 days with different concentrations of AgNPs or AgNO3. A similar increase in root elongation was observed when seedlings were exposed to 10 mg Ag L(1) of either PVP-AgNPs or AgNO3. At this concentration we performed electron microscopy investigations and 2-dimensional electrophoresis (2DE) proteomic profiling. The low level of overlap of differentially expressed proteins indicates that AgNPs and AgNO3 cause different plant responses. Both Ag treatments cause changes in proteins involved in the redox regulation and in the sulfur metabolism. These responses could play an important role to maintain cellular homeostasis. Only the AgNP exposure cause the alteration of some proteins related to the endoplasmic reticulum and vacuole indicating these two organelles as targets of the AgNPs action. These data add further evidences that the effects of AgNPs are not simply due to the release of Ag ions.
format	article
author	Candida Vannini Guido Domingo Elisabetta Onelli Bhakti Prinsi Milena Marsoni Luca Espen Marcella Bracale
author_facet	Candida Vannini Guido Domingo Elisabetta Onelli Bhakti Prinsi Milena Marsoni Luca Espen Marcella Bracale
author_sort	Candida Vannini
title	Morphological and proteomic responses of Eruca sativa exposed to silver nanoparticles or silver nitrate.
title_short	Morphological and proteomic responses of Eruca sativa exposed to silver nanoparticles or silver nitrate.
title_full	Morphological and proteomic responses of Eruca sativa exposed to silver nanoparticles or silver nitrate.
title_fullStr	Morphological and proteomic responses of Eruca sativa exposed to silver nanoparticles or silver nitrate.
title_full_unstemmed	Morphological and proteomic responses of Eruca sativa exposed to silver nanoparticles or silver nitrate.
title_sort	morphological and proteomic responses of eruca sativa exposed to silver nanoparticles or silver nitrate.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/8b74753be7194da8a28d79357d0e8fe1
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Morphological and proteomic responses of Eruca sativa exposed to silver nanoparticles or silver nitrate.

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