A novel technique to determine the cell type specific response within an in vitro co-culture model via multi-colour flow cytometry

Abstract Determination of the cell type specific response is essential towards understanding the cellular mechanisms associated with disease states as well as assessing cell-based targeting of effective therapeutic agents. Recently, there have been increased calls for advanced in vitro multi-cellula...

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Autores principales:	Martin J. D. Clift, Kleanthis Fytianos, Dimitri Vanhecke, Sandra Hočevar, Alke Petri-Fink, Barbara Rothen-Rutishauser
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b74cca3e2d84354a89dcadd9f04aea9
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spelling	oai:doaj.org-article:8b74cca3e2d84354a89dcadd9f04aea92021-12-02T15:05:22ZA novel technique to determine the cell type specific response within an in vitro co-culture model via multi-colour flow cytometry10.1038/s41598-017-00369-42045-2322https://doaj.org/article/8b74cca3e2d84354a89dcadd9f04aea92017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00369-4https://doaj.org/toc/2045-2322Abstract Determination of the cell type specific response is essential towards understanding the cellular mechanisms associated with disease states as well as assessing cell-based targeting of effective therapeutic agents. Recently, there have been increased calls for advanced in vitro multi-cellular models that provide reliable and valuable tools correlative to in vivo. In this pursuit the ability to assess the cell type specific response is imperative. Herein, we report a novel approach towards resolving each specific cell type of a multi-cellular model representing the human lung epithelial tissue barrier via multi-colour flow cytometry (FACS). We proved via ≤ five-colour FACS that the manipulation of this in vitro model allowed each cell type to be resolved with no impact upon cell viability. Subsequently, four-colour FACS verified the ability to determine the biochemical effect (e.g. oxidative stress) of each specific cell type. This technique will be vital in gaining information upon cellular mechanics when using next-level, multi-cellular in vitro strategies.Martin J. D. CliftKleanthis FytianosDimitri VanheckeSandra HočevarAlke Petri-FinkBarbara Rothen-RutishauserNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martin J. D. Clift Kleanthis Fytianos Dimitri Vanhecke Sandra Hočevar Alke Petri-Fink Barbara Rothen-Rutishauser A novel technique to determine the cell type specific response within an in vitro co-culture model via multi-colour flow cytometry
description	Abstract Determination of the cell type specific response is essential towards understanding the cellular mechanisms associated with disease states as well as assessing cell-based targeting of effective therapeutic agents. Recently, there have been increased calls for advanced in vitro multi-cellular models that provide reliable and valuable tools correlative to in vivo. In this pursuit the ability to assess the cell type specific response is imperative. Herein, we report a novel approach towards resolving each specific cell type of a multi-cellular model representing the human lung epithelial tissue barrier via multi-colour flow cytometry (FACS). We proved via ≤ five-colour FACS that the manipulation of this in vitro model allowed each cell type to be resolved with no impact upon cell viability. Subsequently, four-colour FACS verified the ability to determine the biochemical effect (e.g. oxidative stress) of each specific cell type. This technique will be vital in gaining information upon cellular mechanics when using next-level, multi-cellular in vitro strategies.
format	article
author	Martin J. D. Clift Kleanthis Fytianos Dimitri Vanhecke Sandra Hočevar Alke Petri-Fink Barbara Rothen-Rutishauser
author_facet	Martin J. D. Clift Kleanthis Fytianos Dimitri Vanhecke Sandra Hočevar Alke Petri-Fink Barbara Rothen-Rutishauser
author_sort	Martin J. D. Clift
title	A novel technique to determine the cell type specific response within an in vitro co-culture model via multi-colour flow cytometry
title_short	A novel technique to determine the cell type specific response within an in vitro co-culture model via multi-colour flow cytometry
title_full	A novel technique to determine the cell type specific response within an in vitro co-culture model via multi-colour flow cytometry
title_fullStr	A novel technique to determine the cell type specific response within an in vitro co-culture model via multi-colour flow cytometry
title_full_unstemmed	A novel technique to determine the cell type specific response within an in vitro co-culture model via multi-colour flow cytometry
title_sort	novel technique to determine the cell type specific response within an in vitro co-culture model via multi-colour flow cytometry
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8b74cca3e2d84354a89dcadd9f04aea9
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A novel technique to determine the cell type specific response within an in vitro co-culture model via multi-colour flow cytometry

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