Enhanced efficacy of sublingual immunotherapy by liposome-mediated delivery of allergen

Have Aliu,1–3 Carola Rask,1 Jens Brimnes,1 Thomas Lars Andresen2,3 1Immunology Department, In vivo Biology Team, ALK Abelló A/S, Hørsholm, 2Department of Micro- and Nanotechnology, Technical University of Denmark, 3Center for Nanomedicine and Theranostics, Technical...

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Autores principales:	Aliu H, Rask C, Brimnes J, Andresen TL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Sublingual Immunotherapy Drug Delivery Allergy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8b809d56112c4111a4e1ab7c25129a1f
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spelling	oai:doaj.org-article:8b809d56112c4111a4e1ab7c25129a1f2021-12-02T00:07:18ZEnhanced efficacy of sublingual immunotherapy by liposome-mediated delivery of allergen1178-2013https://doaj.org/article/8b809d56112c4111a4e1ab7c25129a1f2017-11-01T00:00:00Zhttps://www.dovepress.com/enhanced-efficacy-of-sublingual-immunotherapy-by-liposome-mediated-del-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Have Aliu,1–3 Carola Rask,1 Jens Brimnes,1 Thomas Lars Andresen2,3 1Immunology Department, In vivo Biology Team, ALK Abelló A/S, Hørsholm, 2Department of Micro- and Nanotechnology, Technical University of Denmark, 3Center for Nanomedicine and Theranostics, Technical University of Denmark, Kgs Lyngby, Denmark Abstract: Immunotherapy by sublingual administration of allergens provides high patient compliance and has emerged as an alternative to subcutaneous immunotherapy for the treatment of IgE-associated allergic diseases. However, sublingual immunotherapy (SLIT) can cause adverse events. Development of allergen delivery systems enabling more efficient delivery and hence lower allergen load might reduce the adverse events. In the present study, we have investigated neutral and cationic liposomes as delivery systems of ovalbumin (OVA), as a model allergen, in an OVA-induced allergic airway inflammation model. We investigated the liposome carriers’ ability to improve tolerance induction of antigens compared to the corresponding dose of free OVA. Mice were treated sublingually over 2 weeks with free or liposome encapsulated OVA followed by intraperitoneal injections and intranasal challenge. Mice sublingually treated with OVA-liposomes showed a significant reduction of airway eosinophilia and splenocyte proliferation in comparison to free OVA. A similar nonsignificant pattern was seen for OVA-specific IgE antibodies. In addition, reduced levels of interferon-γ and interleukin-5 were observed in spleen cell culture supernatants from OVA-liposome-treated mice compared to the sham-treated group. In conclusion, in vivo efficacy data showed that prophylactic SLIT with OVA-liposomes is significantly more effective in preventing allergic inflammation than the corresponding dose of free OVA. Keywords: sublingual immunotherapy, drug delivery, allergy, liposome Aliu HRask CBrimnes JAndresen TLDove Medical PressarticleSublingual ImmunotherapyDrug DeliveryAllergyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 8377-8388 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sublingual Immunotherapy Drug Delivery Allergy Medicine (General) R5-920
spellingShingle	Sublingual Immunotherapy Drug Delivery Allergy Medicine (General) R5-920 Aliu H Rask C Brimnes J Andresen TL Enhanced efficacy of sublingual immunotherapy by liposome-mediated delivery of allergen
description	Have Aliu,1–3 Carola Rask,1 Jens Brimnes,1 Thomas Lars Andresen2,3 1Immunology Department, In vivo Biology Team, ALK Abelló A/S, Hørsholm, 2Department of Micro- and Nanotechnology, Technical University of Denmark, 3Center for Nanomedicine and Theranostics, Technical University of Denmark, Kgs Lyngby, Denmark Abstract: Immunotherapy by sublingual administration of allergens provides high patient compliance and has emerged as an alternative to subcutaneous immunotherapy for the treatment of IgE-associated allergic diseases. However, sublingual immunotherapy (SLIT) can cause adverse events. Development of allergen delivery systems enabling more efficient delivery and hence lower allergen load might reduce the adverse events. In the present study, we have investigated neutral and cationic liposomes as delivery systems of ovalbumin (OVA), as a model allergen, in an OVA-induced allergic airway inflammation model. We investigated the liposome carriers’ ability to improve tolerance induction of antigens compared to the corresponding dose of free OVA. Mice were treated sublingually over 2 weeks with free or liposome encapsulated OVA followed by intraperitoneal injections and intranasal challenge. Mice sublingually treated with OVA-liposomes showed a significant reduction of airway eosinophilia and splenocyte proliferation in comparison to free OVA. A similar nonsignificant pattern was seen for OVA-specific IgE antibodies. In addition, reduced levels of interferon-γ and interleukin-5 were observed in spleen cell culture supernatants from OVA-liposome-treated mice compared to the sham-treated group. In conclusion, in vivo efficacy data showed that prophylactic SLIT with OVA-liposomes is significantly more effective in preventing allergic inflammation than the corresponding dose of free OVA. Keywords: sublingual immunotherapy, drug delivery, allergy, liposome
format	article
author	Aliu H Rask C Brimnes J Andresen TL
author_facet	Aliu H Rask C Brimnes J Andresen TL
author_sort	Aliu H
title	Enhanced efficacy of sublingual immunotherapy by liposome-mediated delivery of allergen
title_short	Enhanced efficacy of sublingual immunotherapy by liposome-mediated delivery of allergen
title_full	Enhanced efficacy of sublingual immunotherapy by liposome-mediated delivery of allergen
title_fullStr	Enhanced efficacy of sublingual immunotherapy by liposome-mediated delivery of allergen
title_full_unstemmed	Enhanced efficacy of sublingual immunotherapy by liposome-mediated delivery of allergen
title_sort	enhanced efficacy of sublingual immunotherapy by liposome-mediated delivery of allergen
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/8b809d56112c4111a4e1ab7c25129a1f
work_keys_str_mv	AT aliuh enhancedefficacyofsublingualimmunotherapybyliposomemediateddeliveryofallergen AT raskc enhancedefficacyofsublingualimmunotherapybyliposomemediateddeliveryofallergen AT brimnesj enhancedefficacyofsublingualimmunotherapybyliposomemediateddeliveryofallergen AT andresentl enhancedefficacyofsublingualimmunotherapybyliposomemediateddeliveryofallergen
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Enhanced efficacy of sublingual immunotherapy by liposome-mediated delivery of allergen

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