Circ_0043532 regulates miR-182/SGK3 axis to promote granulosa cell progression in polycystic ovary syndrome

Abstract Background Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disease in women at childbearing age. Several circular RNAs (circRNAs) have been demonstrated to be involved in PCOS. In this study, we aimed to explore the function and mechanism of circ_0043532 in PCOS. Method...

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Autores principales:	Lishuang Xu, Fang Xiong, Yinyang Bai, Juxia Xiao, Yun Zhang, Jie Chen, Qiuping Li
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	PCOS circ_0043532 miR-182 SGK3 Gynecology and obstetrics RG1-991 Reproduction QH471-489
Acceso en línea:	https://doaj.org/article/8b87e3aa43c041cc9120a85f2e04aa49
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spelling	oai:doaj.org-article:8b87e3aa43c041cc9120a85f2e04aa492021-11-08T11:14:32ZCirc_0043532 regulates miR-182/SGK3 axis to promote granulosa cell progression in polycystic ovary syndrome10.1186/s12958-021-00839-51477-7827https://doaj.org/article/8b87e3aa43c041cc9120a85f2e04aa492021-11-01T00:00:00Zhttps://doi.org/10.1186/s12958-021-00839-5https://doaj.org/toc/1477-7827Abstract Background Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disease in women at childbearing age. Several circular RNAs (circRNAs) have been demonstrated to be involved in PCOS. In this study, we aimed to explore the function and mechanism of circ_0043532 in PCOS. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to determine the expression of circ_0043532, miR-182 and serum/glucocorticoid regulated kinase family member 3 (SGK3). Cell proliferation was assessed by 5-ethynyl-2′-deoxyuridine (EdU) assay and 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Flow cytometry analysis was employed to evaluate cell cycle and cell apoptosis. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were conducted to verify the association between miR-182 and SGK3. Western blot assay was carried out to determine the protein level of SGK3. Results Circ_0043532 was markedly elevated in PCOS granulosa cells (GCs) and KGN cells. Silencing of circ_0043532 suppressed cell proliferation and cell cycle process and promoted cell apoptosis in PCOS GCs and KGN cells. For mechanistic analysis, circ_0043532 was identified as a sponge of miR-182 and SGK3 was confirmed to be a target gene of miR-182. Inhibition of miR-182 rescued the impacts of circ_0043532 interference on PCOS GCs and KGN cell progression. Moreover, miR-182 overexpression suppressed cell proliferation and cell cycle process and promoted cell apoptosis in PCOS GCs and KGN cells by targeting SGK3. Conclusion Deficiency of circ_0043532 suppressed cell proliferation and induced cell cycle arrest and cell apoptosis in PCOS by modulation of miR-182/SGK3 axis.Lishuang XuFang XiongYinyang BaiJuxia XiaoYun ZhangJie ChenQiuping LiBMCarticlePCOScirc_0043532miR-182SGK3Gynecology and obstetricsRG1-991ReproductionQH471-489ENReproductive Biology and Endocrinology, Vol 19, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	PCOS circ_0043532 miR-182 SGK3 Gynecology and obstetrics RG1-991 Reproduction QH471-489
spellingShingle	PCOS circ_0043532 miR-182 SGK3 Gynecology and obstetrics RG1-991 Reproduction QH471-489 Lishuang Xu Fang Xiong Yinyang Bai Juxia Xiao Yun Zhang Jie Chen Qiuping Li Circ_0043532 regulates miR-182/SGK3 axis to promote granulosa cell progression in polycystic ovary syndrome
description	Abstract Background Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disease in women at childbearing age. Several circular RNAs (circRNAs) have been demonstrated to be involved in PCOS. In this study, we aimed to explore the function and mechanism of circ_0043532 in PCOS. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to determine the expression of circ_0043532, miR-182 and serum/glucocorticoid regulated kinase family member 3 (SGK3). Cell proliferation was assessed by 5-ethynyl-2′-deoxyuridine (EdU) assay and 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Flow cytometry analysis was employed to evaluate cell cycle and cell apoptosis. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were conducted to verify the association between miR-182 and SGK3. Western blot assay was carried out to determine the protein level of SGK3. Results Circ_0043532 was markedly elevated in PCOS granulosa cells (GCs) and KGN cells. Silencing of circ_0043532 suppressed cell proliferation and cell cycle process and promoted cell apoptosis in PCOS GCs and KGN cells. For mechanistic analysis, circ_0043532 was identified as a sponge of miR-182 and SGK3 was confirmed to be a target gene of miR-182. Inhibition of miR-182 rescued the impacts of circ_0043532 interference on PCOS GCs and KGN cell progression. Moreover, miR-182 overexpression suppressed cell proliferation and cell cycle process and promoted cell apoptosis in PCOS GCs and KGN cells by targeting SGK3. Conclusion Deficiency of circ_0043532 suppressed cell proliferation and induced cell cycle arrest and cell apoptosis in PCOS by modulation of miR-182/SGK3 axis.
format	article
author	Lishuang Xu Fang Xiong Yinyang Bai Juxia Xiao Yun Zhang Jie Chen Qiuping Li
author_facet	Lishuang Xu Fang Xiong Yinyang Bai Juxia Xiao Yun Zhang Jie Chen Qiuping Li
author_sort	Lishuang Xu
title	Circ_0043532 regulates miR-182/SGK3 axis to promote granulosa cell progression in polycystic ovary syndrome
title_short	Circ_0043532 regulates miR-182/SGK3 axis to promote granulosa cell progression in polycystic ovary syndrome
title_full	Circ_0043532 regulates miR-182/SGK3 axis to promote granulosa cell progression in polycystic ovary syndrome
title_fullStr	Circ_0043532 regulates miR-182/SGK3 axis to promote granulosa cell progression in polycystic ovary syndrome
title_full_unstemmed	Circ_0043532 regulates miR-182/SGK3 axis to promote granulosa cell progression in polycystic ovary syndrome
title_sort	circ_0043532 regulates mir-182/sgk3 axis to promote granulosa cell progression in polycystic ovary syndrome
publisher	BMC
publishDate	2021
url	https://doaj.org/article/8b87e3aa43c041cc9120a85f2e04aa49
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_version_	1718442354978848768

Circ_0043532 regulates miR-182/SGK3 axis to promote granulosa cell progression in polycystic ovary syndrome

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