Functional genetic diversity among Mycobacterium tuberculosis complex clinical isolates: delineation of conserved core and lineage-specific transcriptomes during intracellular survival.

Tuberculosis exerts a tremendous burden on global health, with approximately 9 million new infections and approximately 2 million deaths annually. The Mycobacterium tuberculosis complex (MTC) was initially regarded as a highly homogeneous population; however, recent data suggest the causative agents...

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Autores principales:	Susanne Homolka, Stefan Niemann, David G Russell, Kyle H Rohde
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8b88b0f455d9413da835f18dfca7b6e9
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spelling	oai:doaj.org-article:8b88b0f455d9413da835f18dfca7b6e92021-12-02T20:00:29ZFunctional genetic diversity among Mycobacterium tuberculosis complex clinical isolates: delineation of conserved core and lineage-specific transcriptomes during intracellular survival.1553-73661553-737410.1371/journal.ppat.1000988https://doaj.org/article/8b88b0f455d9413da835f18dfca7b6e92010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20628579/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Tuberculosis exerts a tremendous burden on global health, with approximately 9 million new infections and approximately 2 million deaths annually. The Mycobacterium tuberculosis complex (MTC) was initially regarded as a highly homogeneous population; however, recent data suggest the causative agents of tuberculosis are more genetically and functionally diverse than appreciated previously. The impact of this natural variation on the virulence and clinical manifestations of the pathogen remains largely unknown. This report examines the effect of genetic diversity among MTC clinical isolates on global gene expression and survival within macrophages. We discovered lineage-specific transcription patterns in vitro and distinct intracellular growth profiles associated with specific responses to host-derived environmental cues. Strain comparisons also facilitated delineation of a core intracellular transcriptome, including genes with highly conserved regulation across the global panel of clinical isolates. This study affords new insights into the genetic information that M. tuberculosis has conserved under selective pressure during its long-term interactions with its human host.Susanne HomolkaStefan NiemannDavid G RussellKyle H RohdePublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, Iss 7, p e1000988 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Susanne Homolka Stefan Niemann David G Russell Kyle H Rohde Functional genetic diversity among Mycobacterium tuberculosis complex clinical isolates: delineation of conserved core and lineage-specific transcriptomes during intracellular survival.
description	Tuberculosis exerts a tremendous burden on global health, with approximately 9 million new infections and approximately 2 million deaths annually. The Mycobacterium tuberculosis complex (MTC) was initially regarded as a highly homogeneous population; however, recent data suggest the causative agents of tuberculosis are more genetically and functionally diverse than appreciated previously. The impact of this natural variation on the virulence and clinical manifestations of the pathogen remains largely unknown. This report examines the effect of genetic diversity among MTC clinical isolates on global gene expression and survival within macrophages. We discovered lineage-specific transcription patterns in vitro and distinct intracellular growth profiles associated with specific responses to host-derived environmental cues. Strain comparisons also facilitated delineation of a core intracellular transcriptome, including genes with highly conserved regulation across the global panel of clinical isolates. This study affords new insights into the genetic information that M. tuberculosis has conserved under selective pressure during its long-term interactions with its human host.
format	article
author	Susanne Homolka Stefan Niemann David G Russell Kyle H Rohde
author_facet	Susanne Homolka Stefan Niemann David G Russell Kyle H Rohde
author_sort	Susanne Homolka
title	Functional genetic diversity among Mycobacterium tuberculosis complex clinical isolates: delineation of conserved core and lineage-specific transcriptomes during intracellular survival.
title_short	Functional genetic diversity among Mycobacterium tuberculosis complex clinical isolates: delineation of conserved core and lineage-specific transcriptomes during intracellular survival.
title_full	Functional genetic diversity among Mycobacterium tuberculosis complex clinical isolates: delineation of conserved core and lineage-specific transcriptomes during intracellular survival.
title_fullStr	Functional genetic diversity among Mycobacterium tuberculosis complex clinical isolates: delineation of conserved core and lineage-specific transcriptomes during intracellular survival.
title_full_unstemmed	Functional genetic diversity among Mycobacterium tuberculosis complex clinical isolates: delineation of conserved core and lineage-specific transcriptomes during intracellular survival.
title_sort	functional genetic diversity among mycobacterium tuberculosis complex clinical isolates: delineation of conserved core and lineage-specific transcriptomes during intracellular survival.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/8b88b0f455d9413da835f18dfca7b6e9
work_keys_str_mv	AT susannehomolka functionalgeneticdiversityamongmycobacteriumtuberculosiscomplexclinicalisolatesdelineationofconservedcoreandlineagespecifictranscriptomesduringintracellularsurvival AT stefanniemann functionalgeneticdiversityamongmycobacteriumtuberculosiscomplexclinicalisolatesdelineationofconservedcoreandlineagespecifictranscriptomesduringintracellularsurvival AT davidgrussell functionalgeneticdiversityamongmycobacteriumtuberculosiscomplexclinicalisolatesdelineationofconservedcoreandlineagespecifictranscriptomesduringintracellularsurvival AT kylehrohde functionalgeneticdiversityamongmycobacteriumtuberculosiscomplexclinicalisolatesdelineationofconservedcoreandlineagespecifictranscriptomesduringintracellularsurvival
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Functional genetic diversity among Mycobacterium tuberculosis complex clinical isolates: delineation of conserved core and lineage-specific transcriptomes during intracellular survival.

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