Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver

Oxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chron’s disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antiox...

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Autores principales:	Stojanović Marija, Šćepanović Ljiljana, Bosnić Olivera, Mitrović Dušan, Jozanov-Stankov Olga, Šćepanović Vuk, Šćepanović Radomir, Stojanović Teja, Ilić Slobodan, Djurić Dragan
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2016
Materias:	antioxidant enzymes homocysteine lipid peroxidation oxidative stress Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/8b8a3143e51d4ec49e22d16de7ea9d2b
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spelling	oai:doaj.org-article:8b8a3143e51d4ec49e22d16de7ea9d2b2021-11-17T21:27:50ZEffects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver1820-744810.1515/acve-2016-0002https://doaj.org/article/8b8a3143e51d4ec49e22d16de7ea9d2b2016-03-01T00:00:00Zhttps://doi.org/10.1515/acve-2016-0002https://doaj.org/toc/1820-7448Oxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chron’s disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antioxidant status of rat intestine and liver. The levels of thiobarbituric acid reactive substances (TBARS), activity of catalase (CAT) and total antioxidant status (TAS) were investigated in the isolated gut and liver of young male rats in the control group (8 rats) and after 3-hоur incubation in high doses of D, L-homocysteine thionolactone (Hcy) (10 μmol/L) (8 rats). Samples of duodenum, ileum, colon and liver were homogenized in sodium phosphate buffer (1:10). Homogenates were centrifuged at 10000 for 10 min at 4° C and the supernatant was taken for biochemical assays. Our results showed that high D, L-homocysteine thionolactone concentration reduced enzymatic catalase activity in homogenates of the isolated segments of duodenum (27.04%) p<0.01; ileum (37.27%), colon (34.17%) and liver (67.46%) p<0.001. Exposition to high D,L-homocysteine thiolactone concentration significantly increased TBARS levels in the duodenum (106.05%), ileum (47.24%), colon (112.75%) and liver (32.07%) (p<0.01). Homocysteine also modifi ed the total antioxidant status of homogenates from the duodenum, ileum, colon and liver, increasing by 20.68% (duodenum), 24.74% (ileum), 14.88% (colon) and 19.35% (liver) (p<0.001). Homocysteine induced a consistent oxidative stress in rat’s intestine and liver (reduced activity of catalase and increased level of TBARS), but the elevated activity of TAS in our experiments could be explained as an adaptive response to the generated free radicals which indicates the failure of the total antioxidant defense mechanism to protect the tissues from damage caused by homocysteine.Stojanović MarijaŠćepanović LjiljanaBosnić OliveraMitrović DušanJozanov-Stankov OlgaŠćepanović VukŠćepanović RadomirStojanović TejaIlić SlobodanDjurić DraganSciendoarticleantioxidant enzymeshomocysteinelipid peroxidationoxidative stressVeterinary medicineSF600-1100ENActa Veterinaria, Vol 66, Iss 1, Pp 26-36 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	antioxidant enzymes homocysteine lipid peroxidation oxidative stress Veterinary medicine SF600-1100
spellingShingle	antioxidant enzymes homocysteine lipid peroxidation oxidative stress Veterinary medicine SF600-1100 Stojanović Marija Šćepanović Ljiljana Bosnić Olivera Mitrović Dušan Jozanov-Stankov Olga Šćepanović Vuk Šćepanović Radomir Stojanović Teja Ilić Slobodan Djurić Dragan Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver
description	Oxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chron’s disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antioxidant status of rat intestine and liver. The levels of thiobarbituric acid reactive substances (TBARS), activity of catalase (CAT) and total antioxidant status (TAS) were investigated in the isolated gut and liver of young male rats in the control group (8 rats) and after 3-hоur incubation in high doses of D, L-homocysteine thionolactone (Hcy) (10 μmol/L) (8 rats). Samples of duodenum, ileum, colon and liver were homogenized in sodium phosphate buffer (1:10). Homogenates were centrifuged at 10000 for 10 min at 4° C and the supernatant was taken for biochemical assays. Our results showed that high D, L-homocysteine thionolactone concentration reduced enzymatic catalase activity in homogenates of the isolated segments of duodenum (27.04%) p<0.01; ileum (37.27%), colon (34.17%) and liver (67.46%) p<0.001. Exposition to high D,L-homocysteine thiolactone concentration significantly increased TBARS levels in the duodenum (106.05%), ileum (47.24%), colon (112.75%) and liver (32.07%) (p<0.01). Homocysteine also modifi ed the total antioxidant status of homogenates from the duodenum, ileum, colon and liver, increasing by 20.68% (duodenum), 24.74% (ileum), 14.88% (colon) and 19.35% (liver) (p<0.001). Homocysteine induced a consistent oxidative stress in rat’s intestine and liver (reduced activity of catalase and increased level of TBARS), but the elevated activity of TAS in our experiments could be explained as an adaptive response to the generated free radicals which indicates the failure of the total antioxidant defense mechanism to protect the tissues from damage caused by homocysteine.
format	article
author	Stojanović Marija Šćepanović Ljiljana Bosnić Olivera Mitrović Dušan Jozanov-Stankov Olga Šćepanović Vuk Šćepanović Radomir Stojanović Teja Ilić Slobodan Djurić Dragan
author_facet	Stojanović Marija Šćepanović Ljiljana Bosnić Olivera Mitrović Dušan Jozanov-Stankov Olga Šćepanović Vuk Šćepanović Radomir Stojanović Teja Ilić Slobodan Djurić Dragan
author_sort	Stojanović Marija
title	Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver
title_short	Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver
title_full	Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver
title_fullStr	Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver
title_full_unstemmed	Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver
title_sort	effects of acute administration of d,l-homocysteine thiolactone on the antioxidative status of rat intestine and liver
publisher	Sciendo
publishDate	2016
url	https://doaj.org/article/8b8a3143e51d4ec49e22d16de7ea9d2b
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Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver

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