Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings.

The General Organization of the Veterinary Services in Egypt has adopted a sheeppox vaccination policy to control lumpy skin disease (LSD) in cattle. Over the course of the last two years, recurrent outbreaks were reported, with animals showing severe clinical signs and consequentially higher fatali...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sherin R Rouby, Nesreen M Safwat, Khaled H Hussein, Aml M Abdel-Ra'ouf, Bahaa S Madkour, Ahmed S Abdel-Moneim, Hosein I Hosein
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b8b6a2da6c74535b924d67130273c71
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8b8b6a2da6c74535b924d67130273c71
record_format	dspace
spelling	oai:doaj.org-article:8b8b6a2da6c74535b924d67130273c712021-12-02T20:16:43ZLumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings.1932-620310.1371/journal.pone.0258755https://doaj.org/article/8b8b6a2da6c74535b924d67130273c712021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258755https://doaj.org/toc/1932-6203The General Organization of the Veterinary Services in Egypt has adopted a sheeppox vaccination policy to control lumpy skin disease (LSD) in cattle. Over the course of the last two years, recurrent outbreaks were reported, with animals showing severe clinical signs and consequentially higher fatalities than that of cases reported in previous LSD outbreaks. A total of 1050 cattle showing typical clinical signs suggestive of LSD were clinically and pathologically investigated during 2017-2018. Skin nodules were collected and lumpy skin disease virus (LSDV) was screened in collected skin samples using PCR for the RPO-30 gene. Furthermore, the entire P32 protein coding gene was sequenced. Histopathology and immunohistochemistry of the skin nodules were also conducted. The obtained results showed an overall mortality rate of 6.86%. LSDV was confirmed in all the examined nodules as evidenced by immunohistochemistry and positive PCR amplification of the RPO30 gene. Sequencing analysis of the P32 gene revealed a highly conserved nature and genetic stability of the LSDV. The results of the present study show that the current vaccination protocol was not effective for a multitude of reasons. These results also serve as evidence for a strong recommendation of an amendment of homologous vaccine use aside from a complete coverage of cattle populations in order to reduce the incidence of LSD among cattle population in Egypt.Sherin R RoubyNesreen M SafwatKhaled H HusseinAml M Abdel-Ra'oufBahaa S MadkourAhmed S Abdel-MoneimHosein I HoseinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258755 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sherin R Rouby Nesreen M Safwat Khaled H Hussein Aml M Abdel-Ra'ouf Bahaa S Madkour Ahmed S Abdel-Moneim Hosein I Hosein Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings.
description	The General Organization of the Veterinary Services in Egypt has adopted a sheeppox vaccination policy to control lumpy skin disease (LSD) in cattle. Over the course of the last two years, recurrent outbreaks were reported, with animals showing severe clinical signs and consequentially higher fatalities than that of cases reported in previous LSD outbreaks. A total of 1050 cattle showing typical clinical signs suggestive of LSD were clinically and pathologically investigated during 2017-2018. Skin nodules were collected and lumpy skin disease virus (LSDV) was screened in collected skin samples using PCR for the RPO-30 gene. Furthermore, the entire P32 protein coding gene was sequenced. Histopathology and immunohistochemistry of the skin nodules were also conducted. The obtained results showed an overall mortality rate of 6.86%. LSDV was confirmed in all the examined nodules as evidenced by immunohistochemistry and positive PCR amplification of the RPO30 gene. Sequencing analysis of the P32 gene revealed a highly conserved nature and genetic stability of the LSDV. The results of the present study show that the current vaccination protocol was not effective for a multitude of reasons. These results also serve as evidence for a strong recommendation of an amendment of homologous vaccine use aside from a complete coverage of cattle populations in order to reduce the incidence of LSD among cattle population in Egypt.
format	article
author	Sherin R Rouby Nesreen M Safwat Khaled H Hussein Aml M Abdel-Ra'ouf Bahaa S Madkour Ahmed S Abdel-Moneim Hosein I Hosein
author_facet	Sherin R Rouby Nesreen M Safwat Khaled H Hussein Aml M Abdel-Ra'ouf Bahaa S Madkour Ahmed S Abdel-Moneim Hosein I Hosein
author_sort	Sherin R Rouby
title	Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings.
title_short	Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings.
title_full	Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings.
title_fullStr	Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings.
title_full_unstemmed	Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings.
title_sort	lumpy skin disease outbreaks in egypt during 2017-2018 among sheeppox vaccinated cattle: epidemiological, pathological, and molecular findings.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/8b8b6a2da6c74535b924d67130273c71
work_keys_str_mv	AT sherinrrouby lumpyskindiseaseoutbreaksinegyptduring20172018amongsheeppoxvaccinatedcattleepidemiologicalpathologicalandmolecularfindings AT nesreenmsafwat lumpyskindiseaseoutbreaksinegyptduring20172018amongsheeppoxvaccinatedcattleepidemiologicalpathologicalandmolecularfindings AT khaledhhussein lumpyskindiseaseoutbreaksinegyptduring20172018amongsheeppoxvaccinatedcattleepidemiologicalpathologicalandmolecularfindings AT amlmabdelraouf lumpyskindiseaseoutbreaksinegyptduring20172018amongsheeppoxvaccinatedcattleepidemiologicalpathologicalandmolecularfindings AT bahaasmadkour lumpyskindiseaseoutbreaksinegyptduring20172018amongsheeppoxvaccinatedcattleepidemiologicalpathologicalandmolecularfindings AT ahmedsabdelmoneim lumpyskindiseaseoutbreaksinegyptduring20172018amongsheeppoxvaccinatedcattleepidemiologicalpathologicalandmolecularfindings AT hoseinihosein lumpyskindiseaseoutbreaksinegyptduring20172018amongsheeppoxvaccinatedcattleepidemiologicalpathologicalandmolecularfindings
_version_	1718374479563849728

Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings.

Ejemplares similares