99mTc-labeled bevacizumab for detecting atherosclerotic plaque linked to plaque neovascularization and monitoring antiangiogenic effects of atorvastatin treatment in ApoE−/− mice

Abstract Atherosclerotic neovascularization plays a significant role in plaque instability as it provides additional lipids and inflammatory mediators to lesions, and resulting in intraplaque hemorrhage. Vascular endothelial growth factor-A (VEGF-A) is considered the predominant proangiogenic factor...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hui Tan, Jun Zhou, Xiangdong Yang, Mieradilijiang Abudupataer, Xiao Li, Yan Hu, Jie Xiao, Hongcheng Shi, Dengfeng Cheng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b99ec0986c84f2db562a8e36e0d0091
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8b99ec0986c84f2db562a8e36e0d0091
record_format	dspace
spelling	oai:doaj.org-article:8b99ec0986c84f2db562a8e36e0d00912021-12-02T15:05:38Z99mTc-labeled bevacizumab for detecting atherosclerotic plaque linked to plaque neovascularization and monitoring antiangiogenic effects of atorvastatin treatment in ApoE−/− mice10.1038/s41598-017-03276-w2045-2322https://doaj.org/article/8b99ec0986c84f2db562a8e36e0d00912017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03276-whttps://doaj.org/toc/2045-2322Abstract Atherosclerotic neovascularization plays a significant role in plaque instability as it provides additional lipids and inflammatory mediators to lesions, and resulting in intraplaque hemorrhage. Vascular endothelial growth factor-A (VEGF-A) is considered the predominant proangiogenic factor in angiogenesis. Bevacizumab, a humanized monoclonal antibody, specifically binds to all VEGF-A isoforms with high affinity. Therefore, in this study, we designed 99mTc-MAG3-bevacizumab as a probe, and then investigated its usefulness as a new imaging agent for the detection of plaque neovessels, while also assessing the therapeutic effect of atorvastatin treatment. The ApoE−/− mice treated with atorvastatin were used as the treatment group, and C57BL/6 J mice were selected as the control group. 99mTc-MAG3-bevacizumab uptake was visualized on atherosclerotic lesions by non-invasive in-vivo micro-SPECT/CT and ex-vivo BSGI planar imaging. The value of P/B in each part of the aorta of ApoE−/− mice was higher than in the treatment group and the C57BL/6 J mice, which was confirmed by Oil Red O staining, CD31 staining and VEGF immunohistochemistry staining. 99mTc-MAG3-bevacizumab imaging allowed for the non-invasive diagnosis and assessment of plaque neovascularization. Furthermore, this probe may be used as a new molecular imaging agent to assess the antiangiogenic effect of atorvastatin.Hui TanJun ZhouXiangdong YangMieradilijiang AbudupataerXiao LiYan HuJie XiaoHongcheng ShiDengfeng ChengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hui Tan Jun Zhou Xiangdong Yang Mieradilijiang Abudupataer Xiao Li Yan Hu Jie Xiao Hongcheng Shi Dengfeng Cheng 99mTc-labeled bevacizumab for detecting atherosclerotic plaque linked to plaque neovascularization and monitoring antiangiogenic effects of atorvastatin treatment in ApoE−/− mice
description	Abstract Atherosclerotic neovascularization plays a significant role in plaque instability as it provides additional lipids and inflammatory mediators to lesions, and resulting in intraplaque hemorrhage. Vascular endothelial growth factor-A (VEGF-A) is considered the predominant proangiogenic factor in angiogenesis. Bevacizumab, a humanized monoclonal antibody, specifically binds to all VEGF-A isoforms with high affinity. Therefore, in this study, we designed 99mTc-MAG3-bevacizumab as a probe, and then investigated its usefulness as a new imaging agent for the detection of plaque neovessels, while also assessing the therapeutic effect of atorvastatin treatment. The ApoE−/− mice treated with atorvastatin were used as the treatment group, and C57BL/6 J mice were selected as the control group. 99mTc-MAG3-bevacizumab uptake was visualized on atherosclerotic lesions by non-invasive in-vivo micro-SPECT/CT and ex-vivo BSGI planar imaging. The value of P/B in each part of the aorta of ApoE−/− mice was higher than in the treatment group and the C57BL/6 J mice, which was confirmed by Oil Red O staining, CD31 staining and VEGF immunohistochemistry staining. 99mTc-MAG3-bevacizumab imaging allowed for the non-invasive diagnosis and assessment of plaque neovascularization. Furthermore, this probe may be used as a new molecular imaging agent to assess the antiangiogenic effect of atorvastatin.
format	article
author	Hui Tan Jun Zhou Xiangdong Yang Mieradilijiang Abudupataer Xiao Li Yan Hu Jie Xiao Hongcheng Shi Dengfeng Cheng
author_facet	Hui Tan Jun Zhou Xiangdong Yang Mieradilijiang Abudupataer Xiao Li Yan Hu Jie Xiao Hongcheng Shi Dengfeng Cheng
author_sort	Hui Tan
title	99mTc-labeled bevacizumab for detecting atherosclerotic plaque linked to plaque neovascularization and monitoring antiangiogenic effects of atorvastatin treatment in ApoE−/− mice
title_short	99mTc-labeled bevacizumab for detecting atherosclerotic plaque linked to plaque neovascularization and monitoring antiangiogenic effects of atorvastatin treatment in ApoE−/− mice
title_full	99mTc-labeled bevacizumab for detecting atherosclerotic plaque linked to plaque neovascularization and monitoring antiangiogenic effects of atorvastatin treatment in ApoE−/− mice
title_fullStr	99mTc-labeled bevacizumab for detecting atherosclerotic plaque linked to plaque neovascularization and monitoring antiangiogenic effects of atorvastatin treatment in ApoE−/− mice
title_full_unstemmed	99mTc-labeled bevacizumab for detecting atherosclerotic plaque linked to plaque neovascularization and monitoring antiangiogenic effects of atorvastatin treatment in ApoE−/− mice
title_sort	99mtc-labeled bevacizumab for detecting atherosclerotic plaque linked to plaque neovascularization and monitoring antiangiogenic effects of atorvastatin treatment in apoe−/− mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8b99ec0986c84f2db562a8e36e0d0091
work_keys_str_mv	AT huitan 99mtclabeledbevacizumabfordetectingatheroscleroticplaquelinkedtoplaqueneovascularizationandmonitoringantiangiogeniceffectsofatorvastatintreatmentinapoemice AT junzhou 99mtclabeledbevacizumabfordetectingatheroscleroticplaquelinkedtoplaqueneovascularizationandmonitoringantiangiogeniceffectsofatorvastatintreatmentinapoemice AT xiangdongyang 99mtclabeledbevacizumabfordetectingatheroscleroticplaquelinkedtoplaqueneovascularizationandmonitoringantiangiogeniceffectsofatorvastatintreatmentinapoemice AT mieradilijiangabudupataer 99mtclabeledbevacizumabfordetectingatheroscleroticplaquelinkedtoplaqueneovascularizationandmonitoringantiangiogeniceffectsofatorvastatintreatmentinapoemice AT xiaoli 99mtclabeledbevacizumabfordetectingatheroscleroticplaquelinkedtoplaqueneovascularizationandmonitoringantiangiogeniceffectsofatorvastatintreatmentinapoemice AT yanhu 99mtclabeledbevacizumabfordetectingatheroscleroticplaquelinkedtoplaqueneovascularizationandmonitoringantiangiogeniceffectsofatorvastatintreatmentinapoemice AT jiexiao 99mtclabeledbevacizumabfordetectingatheroscleroticplaquelinkedtoplaqueneovascularizationandmonitoringantiangiogeniceffectsofatorvastatintreatmentinapoemice AT hongchengshi 99mtclabeledbevacizumabfordetectingatheroscleroticplaquelinkedtoplaqueneovascularizationandmonitoringantiangiogeniceffectsofatorvastatintreatmentinapoemice AT dengfengcheng 99mtclabeledbevacizumabfordetectingatheroscleroticplaquelinkedtoplaqueneovascularizationandmonitoringantiangiogeniceffectsofatorvastatintreatmentinapoemice
_version_	1718388777880125440

99mTc-labeled bevacizumab for detecting atherosclerotic plaque linked to plaque neovascularization and monitoring antiangiogenic effects of atorvastatin treatment in ApoE−/− mice

Ejemplares similares