High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing.
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High-Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. In the present proto...
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Public Library of Science (PLoS)
2010
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oai:doaj.org-article:8b9d9dcfeb534fb789af35cbb6b549d12021-11-18T06:36:22ZHigh-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing.1932-620310.1371/journal.pone.0012010https://doaj.org/article/8b9d9dcfeb534fb789af35cbb6b549d12010-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20700453/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High-Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. In the present protocol, index (barcode) sequences are employed to discriminate tags from different samples. Such barcodes allow researchers to analyze digital tags from transcriptomes of many samples in a single sequencing run by simply pooling the libraries. Here, we demonstrated that HT-SuperSAGE provided highly sensitive, reproducible and accurate digital gene expression data. By increasing throughput for analysis in HT-SuperSAGE, various applications are foreseen and several examples are provided in the present study, including analyses of laser-microdissected cells, biological replicates and tag extraction using different anchoring enzymes.Hideo MatsumuraKentaro YoshidaShujun LuoEiji KimuraTakahiro FujibeZayed AlbertynRoberto A BarreroDetlev H KrügerGünter KahlGary P SchrothRyohei TerauchiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 8, p e12010 (2010) |
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| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Hideo Matsumura Kentaro Yoshida Shujun Luo Eiji Kimura Takahiro Fujibe Zayed Albertyn Roberto A Barrero Detlev H Krüger Günter Kahl Gary P Schroth Ryohei Terauchi High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing. |
| description |
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High-Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. In the present protocol, index (barcode) sequences are employed to discriminate tags from different samples. Such barcodes allow researchers to analyze digital tags from transcriptomes of many samples in a single sequencing run by simply pooling the libraries. Here, we demonstrated that HT-SuperSAGE provided highly sensitive, reproducible and accurate digital gene expression data. By increasing throughput for analysis in HT-SuperSAGE, various applications are foreseen and several examples are provided in the present study, including analyses of laser-microdissected cells, biological replicates and tag extraction using different anchoring enzymes. |
| format |
article |
| author |
Hideo Matsumura Kentaro Yoshida Shujun Luo Eiji Kimura Takahiro Fujibe Zayed Albertyn Roberto A Barrero Detlev H Krüger Günter Kahl Gary P Schroth Ryohei Terauchi |
| author_facet |
Hideo Matsumura Kentaro Yoshida Shujun Luo Eiji Kimura Takahiro Fujibe Zayed Albertyn Roberto A Barrero Detlev H Krüger Günter Kahl Gary P Schroth Ryohei Terauchi |
| author_sort |
Hideo Matsumura |
| title |
High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing. |
| title_short |
High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing. |
| title_full |
High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing. |
| title_fullStr |
High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing. |
| title_full_unstemmed |
High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing. |
| title_sort |
high-throughput supersage for digital gene expression analysis of multiple samples using next generation sequencing. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2010 |
| url |
https://doaj.org/article/8b9d9dcfeb534fb789af35cbb6b549d1 |
| work_keys_str_mv |
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