Research on the stability of a rabbit dry eye model induced by topical application of the preservative benzalkonium chloride.

<h4>Background</h4>Dry eye is a common disease worldwide, and animal models are critical for the study of it. At present, there is no research about the stability of the extant animal models, which may have negative implications for previous dry eye studies. In this study, we observed th...

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Autores principales:	Chaoyang Li, Yiyue Song, Shaohong Luan, Pengxia Wan, Naiyang Li, Jing Tang, Yu Han, Cuiju Xiong, Zhichong Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b9ded2e31dd451d8f0162bc560224bf
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spelling	oai:doaj.org-article:8b9ded2e31dd451d8f0162bc560224bf2021-11-18T07:24:55ZResearch on the stability of a rabbit dry eye model induced by topical application of the preservative benzalkonium chloride.1932-620310.1371/journal.pone.0033688https://doaj.org/article/8b9ded2e31dd451d8f0162bc560224bf2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22438984/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Dry eye is a common disease worldwide, and animal models are critical for the study of it. At present, there is no research about the stability of the extant animal models, which may have negative implications for previous dry eye studies. In this study, we observed the stability of a rabbit dry eye model induced by the topical benzalkonium chloride (BAC) and determined the valid time of this model.<h4>Methods and findings</h4>Eighty white rabbits were randomly divided into four groups. One eye from each rabbit was randomly chosen to receive topical 0.1% BAC twice daily for 2 weeks (Group BAC-W2), 3 weeks (Group BAC-W3), 4 weeks (Group BAC-W4), or 5 weeks (Group BAC-W5). Fluorescein staining, Schirmer's tests, and conjunctival impression cytology were performed before BAC treatment (normal) and on days 0, 7, 14 and 21 after BAC removal. The eyeballs were collected at these time points for immunofluorescence staining, hematoxylin and eosin (HE) staining, and electron microscopy. After removing BAC, the signs of dry eye in Group BAC-W2 lasted one week. Compared with normal, there were still significant differences in the results of Schirmer's tests and fluorescein staining in Groups BAC-W3 and BAC-W4 on day 7 (P<0.05) and in Group BAC-W5 on day 14 (P<0.05). Decreases in goblet cell density remained stable in the three experimental groups at all time points (P<0.001). Decreased levels of mucin-5 subtype AC (MUC5AC), along with histopathological and ultrastructural disorders of the cornea and conjunctiva could be observed in Group BAC-W4 and particularly in Group BAC-W5 until day 21.<h4>Conclusions</h4>A stable rabbit dry eye model was induced by topical 0.1% BAC for 5 weeks, and after BAC removal, the signs of dry eye were sustained for 2 weeks (for the mixed type of dry eye) or for at least 3 weeks (for mucin-deficient dry eye).Chaoyang LiYiyue SongShaohong LuanPengxia WanNaiyang LiJing TangYu HanCuiju XiongZhichong WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e33688 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chaoyang Li Yiyue Song Shaohong Luan Pengxia Wan Naiyang Li Jing Tang Yu Han Cuiju Xiong Zhichong Wang Research on the stability of a rabbit dry eye model induced by topical application of the preservative benzalkonium chloride.
description	<h4>Background</h4>Dry eye is a common disease worldwide, and animal models are critical for the study of it. At present, there is no research about the stability of the extant animal models, which may have negative implications for previous dry eye studies. In this study, we observed the stability of a rabbit dry eye model induced by the topical benzalkonium chloride (BAC) and determined the valid time of this model.<h4>Methods and findings</h4>Eighty white rabbits were randomly divided into four groups. One eye from each rabbit was randomly chosen to receive topical 0.1% BAC twice daily for 2 weeks (Group BAC-W2), 3 weeks (Group BAC-W3), 4 weeks (Group BAC-W4), or 5 weeks (Group BAC-W5). Fluorescein staining, Schirmer's tests, and conjunctival impression cytology were performed before BAC treatment (normal) and on days 0, 7, 14 and 21 after BAC removal. The eyeballs were collected at these time points for immunofluorescence staining, hematoxylin and eosin (HE) staining, and electron microscopy. After removing BAC, the signs of dry eye in Group BAC-W2 lasted one week. Compared with normal, there were still significant differences in the results of Schirmer's tests and fluorescein staining in Groups BAC-W3 and BAC-W4 on day 7 (P<0.05) and in Group BAC-W5 on day 14 (P<0.05). Decreases in goblet cell density remained stable in the three experimental groups at all time points (P<0.001). Decreased levels of mucin-5 subtype AC (MUC5AC), along with histopathological and ultrastructural disorders of the cornea and conjunctiva could be observed in Group BAC-W4 and particularly in Group BAC-W5 until day 21.<h4>Conclusions</h4>A stable rabbit dry eye model was induced by topical 0.1% BAC for 5 weeks, and after BAC removal, the signs of dry eye were sustained for 2 weeks (for the mixed type of dry eye) or for at least 3 weeks (for mucin-deficient dry eye).
format	article
author	Chaoyang Li Yiyue Song Shaohong Luan Pengxia Wan Naiyang Li Jing Tang Yu Han Cuiju Xiong Zhichong Wang
author_facet	Chaoyang Li Yiyue Song Shaohong Luan Pengxia Wan Naiyang Li Jing Tang Yu Han Cuiju Xiong Zhichong Wang
author_sort	Chaoyang Li
title	Research on the stability of a rabbit dry eye model induced by topical application of the preservative benzalkonium chloride.
title_short	Research on the stability of a rabbit dry eye model induced by topical application of the preservative benzalkonium chloride.
title_full	Research on the stability of a rabbit dry eye model induced by topical application of the preservative benzalkonium chloride.
title_fullStr	Research on the stability of a rabbit dry eye model induced by topical application of the preservative benzalkonium chloride.
title_full_unstemmed	Research on the stability of a rabbit dry eye model induced by topical application of the preservative benzalkonium chloride.
title_sort	research on the stability of a rabbit dry eye model induced by topical application of the preservative benzalkonium chloride.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/8b9ded2e31dd451d8f0162bc560224bf
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Research on the stability of a rabbit dry eye model induced by topical application of the preservative benzalkonium chloride.

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