Metabolic engineering using iterative self-cloning to improve lipid productivity in Coccomyxa

Abstract We previously developed a self-cloning system that introduces cDNA of the uridine monophosphate synthase gene (cUMPS) of Coccomyxa sp. strain Obi as a selectable marker into uracil-auxotrophic mutants (Ura−) of the same alga. Here, we developed a Cre/loxP-based system for the removal of cUM...

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Autores principales: Yuki Kasai, Takuya Tsukahara, Fukiko Ikeda, Yoko Ide, Shigeaki Harayama
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/8ba167cf36e2418999530200e8cb775f
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