Pre-basic seed potato (Solanum tuberosum L.) production using temporary immersion bioreactors

Potato is an important food and cash crop in Perú. Its production is limited due to seed quality, and other biotic and abiotic factors. Here we explore a new alternative for potato pre-basic seed production named temporary immersion bioreactor (TIB). Potato microtubers production using TIB comprises...

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Autores principales: M.de L. Tapia, C. Arbizu, F. Beraún, Lorenzo J., M. Escalona
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Lenguaje:EN
Publicado: Universidad Nacional Agraria La Molina 2018
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Acceso en línea:https://doaj.org/article/8ba258d09a204981ba443310c9bef34f
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spelling oai:doaj.org-article:8ba258d09a204981ba443310c9bef34f2021-12-04T17:28:04ZPre-basic seed potato (Solanum tuberosum L.) production using temporary immersion bioreactors2616-447710.21704/pja.v2i1.1127https://doaj.org/article/8ba258d09a204981ba443310c9bef34f2018-04-01T00:00:00Zhttps://revistas.lamolina.edu.pe/index.php/jpagronomy/article/view/1127https://doaj.org/toc/2616-4477Potato is an important food and cash crop in Perú. Its production is limited due to seed quality, and other biotic and abiotic factors. Here we explore a new alternative for potato pre-basic seed production named temporary immersion bioreactor (TIB). Potato microtubers production using TIB comprises two phases: multiplication and microtuberization. During the multiplication phase, we used 50 segments of four nodes from a semi-solid medium with an immersion and frequency time of five minutes and three hours, respectively. In addition, a photoperiod of 16 hours of light and eight hours of darkness during 28 days were employed. For the microtuberization phase and under darkness conditions during 60 days, we used 80 g/l of sucrose and 30 ml/explant as a volume for the medium. Then, it was possible to obtain five, six, and 2.5 microtubers per explant of potato varieties “Canchan INIA”, “Capiro”, and “Papa 3”, respectively.M.de L. TapiaC. ArbizuF. BeraúnLorenzo J.M. EscalonaUniversidad Nacional Agraria La Molinaarticlemicrotuberstemporary immersion bioreactorpre-basic seedmicrotuberization.Agriculture (General)S1-972ENPeruvian Journal of Agronomy, Vol 2, Iss 1, Pp 9-14 (2018)
institution DOAJ
collection DOAJ
language EN
topic microtubers
temporary immersion bioreactor
pre-basic seed
microtuberization.
Agriculture (General)
S1-972
spellingShingle microtubers
temporary immersion bioreactor
pre-basic seed
microtuberization.
Agriculture (General)
S1-972
M.de L. Tapia
C. Arbizu
F. Beraún
Lorenzo J.
M. Escalona
Pre-basic seed potato (Solanum tuberosum L.) production using temporary immersion bioreactors
description Potato is an important food and cash crop in Perú. Its production is limited due to seed quality, and other biotic and abiotic factors. Here we explore a new alternative for potato pre-basic seed production named temporary immersion bioreactor (TIB). Potato microtubers production using TIB comprises two phases: multiplication and microtuberization. During the multiplication phase, we used 50 segments of four nodes from a semi-solid medium with an immersion and frequency time of five minutes and three hours, respectively. In addition, a photoperiod of 16 hours of light and eight hours of darkness during 28 days were employed. For the microtuberization phase and under darkness conditions during 60 days, we used 80 g/l of sucrose and 30 ml/explant as a volume for the medium. Then, it was possible to obtain five, six, and 2.5 microtubers per explant of potato varieties “Canchan INIA”, “Capiro”, and “Papa 3”, respectively.
format article
author M.de L. Tapia
C. Arbizu
F. Beraún
Lorenzo J.
M. Escalona
author_facet M.de L. Tapia
C. Arbizu
F. Beraún
Lorenzo J.
M. Escalona
author_sort M.de L. Tapia
title Pre-basic seed potato (Solanum tuberosum L.) production using temporary immersion bioreactors
title_short Pre-basic seed potato (Solanum tuberosum L.) production using temporary immersion bioreactors
title_full Pre-basic seed potato (Solanum tuberosum L.) production using temporary immersion bioreactors
title_fullStr Pre-basic seed potato (Solanum tuberosum L.) production using temporary immersion bioreactors
title_full_unstemmed Pre-basic seed potato (Solanum tuberosum L.) production using temporary immersion bioreactors
title_sort pre-basic seed potato (solanum tuberosum l.) production using temporary immersion bioreactors
publisher Universidad Nacional Agraria La Molina
publishDate 2018
url https://doaj.org/article/8ba258d09a204981ba443310c9bef34f
work_keys_str_mv AT mdeltapia prebasicseedpotatosolanumtuberosumlproductionusingtemporaryimmersionbioreactors
AT carbizu prebasicseedpotatosolanumtuberosumlproductionusingtemporaryimmersionbioreactors
AT fberaun prebasicseedpotatosolanumtuberosumlproductionusingtemporaryimmersionbioreactors
AT lorenzoj prebasicseedpotatosolanumtuberosumlproductionusingtemporaryimmersionbioreactors
AT mescalona prebasicseedpotatosolanumtuberosumlproductionusingtemporaryimmersionbioreactors
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