Renoprotective effect of human umbilical cord-derived mesenchymal stem cells in immunodeficient mice suffering from acute kidney injury.

It is unknown whether human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) can improve the renal function of patients suffering from acute kidney injury. Moreover, before beginning clinical trials, it is necessary to investigate this renoprotective effect of hUC-MSCs in a xenogeneic model...

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Autores principales:	Te-Chao Fang, Cheng-Yoong Pang, Sheng-Chun Chiu, Dah-Ching Ding, Rong-Kung Tsai
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8ba299dfcdb446d79d5a9b359818e3a8
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spelling	oai:doaj.org-article:8ba299dfcdb446d79d5a9b359818e3a82021-11-18T08:13:44ZRenoprotective effect of human umbilical cord-derived mesenchymal stem cells in immunodeficient mice suffering from acute kidney injury.1932-620310.1371/journal.pone.0046504https://doaj.org/article/8ba299dfcdb446d79d5a9b359818e3a82012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23029541/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203It is unknown whether human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) can improve the renal function of patients suffering from acute kidney injury. Moreover, before beginning clinical trials, it is necessary to investigate this renoprotective effect of hUC-MSCs in a xenogeneic model of acute kidney injury. However, no previous studies have examined the application of hUC-MSCs to immunodeficient mice suffering from acute kidney injury. The objectives of this study were to examine whether hUC-MSCs could improve renal function in nonobese diabetic-severe combined immune deficiency (NOD-SCID) mice suffering from acute kidney injury, and to investigate the mechanism(s) for hUC-MSCs to improve renal function in this xenogeneic model. Early (3 hr) and late (12 hr) administrations of hUC-MSCs (10(6) cells) were performed via the external jugular vein into NOD-SCID mice suffering from either folic acid (FA) (250 mg/kg body weight) or vehicle. The results showed that early administration of hUC-MSCs improved the renal function of NOD-SCID mice suffering from FA-induced acute kidney injury, as evidenced by decreased serum urea nitrogen and serum creatinine levels, as well as a reduced tubular injury score. The beneficial effects of hUC-MSCs were through reducing apoptosis and promoting proliferation of renal tubular cells. These benefits were independent of inflammatory cytokine effects and transdifferentiation. Furthermore, this study is the first one to show that the reduced apoptosis of renal tubular cells by hUC-MSCs in this xenogeneic model is mediated through the mitochondrial pathway, and through the increase of Akt phosphorylation.Te-Chao FangCheng-Yoong PangSheng-Chun ChiuDah-Ching DingRong-Kung TsaiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e46504 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Te-Chao Fang Cheng-Yoong Pang Sheng-Chun Chiu Dah-Ching Ding Rong-Kung Tsai Renoprotective effect of human umbilical cord-derived mesenchymal stem cells in immunodeficient mice suffering from acute kidney injury.
description	It is unknown whether human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) can improve the renal function of patients suffering from acute kidney injury. Moreover, before beginning clinical trials, it is necessary to investigate this renoprotective effect of hUC-MSCs in a xenogeneic model of acute kidney injury. However, no previous studies have examined the application of hUC-MSCs to immunodeficient mice suffering from acute kidney injury. The objectives of this study were to examine whether hUC-MSCs could improve renal function in nonobese diabetic-severe combined immune deficiency (NOD-SCID) mice suffering from acute kidney injury, and to investigate the mechanism(s) for hUC-MSCs to improve renal function in this xenogeneic model. Early (3 hr) and late (12 hr) administrations of hUC-MSCs (10(6) cells) were performed via the external jugular vein into NOD-SCID mice suffering from either folic acid (FA) (250 mg/kg body weight) or vehicle. The results showed that early administration of hUC-MSCs improved the renal function of NOD-SCID mice suffering from FA-induced acute kidney injury, as evidenced by decreased serum urea nitrogen and serum creatinine levels, as well as a reduced tubular injury score. The beneficial effects of hUC-MSCs were through reducing apoptosis and promoting proliferation of renal tubular cells. These benefits were independent of inflammatory cytokine effects and transdifferentiation. Furthermore, this study is the first one to show that the reduced apoptosis of renal tubular cells by hUC-MSCs in this xenogeneic model is mediated through the mitochondrial pathway, and through the increase of Akt phosphorylation.
format	article
author	Te-Chao Fang Cheng-Yoong Pang Sheng-Chun Chiu Dah-Ching Ding Rong-Kung Tsai
author_facet	Te-Chao Fang Cheng-Yoong Pang Sheng-Chun Chiu Dah-Ching Ding Rong-Kung Tsai
author_sort	Te-Chao Fang
title	Renoprotective effect of human umbilical cord-derived mesenchymal stem cells in immunodeficient mice suffering from acute kidney injury.
title_short	Renoprotective effect of human umbilical cord-derived mesenchymal stem cells in immunodeficient mice suffering from acute kidney injury.
title_full	Renoprotective effect of human umbilical cord-derived mesenchymal stem cells in immunodeficient mice suffering from acute kidney injury.
title_fullStr	Renoprotective effect of human umbilical cord-derived mesenchymal stem cells in immunodeficient mice suffering from acute kidney injury.
title_full_unstemmed	Renoprotective effect of human umbilical cord-derived mesenchymal stem cells in immunodeficient mice suffering from acute kidney injury.
title_sort	renoprotective effect of human umbilical cord-derived mesenchymal stem cells in immunodeficient mice suffering from acute kidney injury.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/8ba299dfcdb446d79d5a9b359818e3a8
work_keys_str_mv	AT techaofang renoprotectiveeffectofhumanumbilicalcordderivedmesenchymalstemcellsinimmunodeficientmicesufferingfromacutekidneyinjury AT chengyoongpang renoprotectiveeffectofhumanumbilicalcordderivedmesenchymalstemcellsinimmunodeficientmicesufferingfromacutekidneyinjury AT shengchunchiu renoprotectiveeffectofhumanumbilicalcordderivedmesenchymalstemcellsinimmunodeficientmicesufferingfromacutekidneyinjury AT dahchingding renoprotectiveeffectofhumanumbilicalcordderivedmesenchymalstemcellsinimmunodeficientmicesufferingfromacutekidneyinjury AT rongkungtsai renoprotectiveeffectofhumanumbilicalcordderivedmesenchymalstemcellsinimmunodeficientmicesufferingfromacutekidneyinjury
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Renoprotective effect of human umbilical cord-derived mesenchymal stem cells in immunodeficient mice suffering from acute kidney injury.

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