Chemerin Regulates Epithelial Barrier Function of Mammary Glands in Dairy Cows

Epithelial barrier function in the mammary gland acts as a forefront of the defense mechanism against mastitis, which is widespread and a major disorder in dairy production. Chemerin is a chemoattractant protein with potent antimicrobial ability, but its role in the mammary gland remains unelucidate...

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Autores principales:	Yutaka Suzuki, Sachi Chiba, Koki Nishihara, Keiichi Nakajima, Akihiko Hagino, Won-Seob Kim, Hong-Gu Lee, Tomonori Nochi, Toru Suzuki, Sang-Gun Roh
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	mammary gland epithelial barrier mastitis chemerin Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/8baa7f87c1dc41c88f9bce5a70c5f683
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spelling	oai:doaj.org-article:8baa7f87c1dc41c88f9bce5a70c5f6832021-11-25T16:18:49ZChemerin Regulates Epithelial Barrier Function of Mammary Glands in Dairy Cows10.3390/ani111131942076-2615https://doaj.org/article/8baa7f87c1dc41c88f9bce5a70c5f6832021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3194https://doaj.org/toc/2076-2615Epithelial barrier function in the mammary gland acts as a forefront of the defense mechanism against mastitis, which is widespread and a major disorder in dairy production. Chemerin is a chemoattractant protein with potent antimicrobial ability, but its role in the mammary gland remains unelucidated. The aim of this study was to determine the function of chemerin in mammary epithelial tissue of dairy cows in lactation or dry-off periods. Mammary epithelial cells produced chemerin protein, and secreted chemerin was detected in milk samples. Chemerin treatment promoted the proliferation of cultured bovine mammary epithelial cells and protected the integrity of the epithelial cell layer from hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-induced damage. Meanwhile, chemerin levels were higher in mammary tissue with mastitis. Tumor necrosis factor alpha (TNF-α) strongly upregulated the expression of the chemerin-coding gene (<i>RARRES2</i>) in mammary epithelial cells. Therefore, chemerin was suggested to support mammary epithelial cell growth and epithelial barrier function and to be regulated by inflammatory stimuli. Our results may indicate chemerin as a novel therapeutic target for diseases in the bovine mammary gland.Yutaka SuzukiSachi ChibaKoki NishiharaKeiichi NakajimaAkihiko HaginoWon-Seob KimHong-Gu LeeTomonori NochiToru SuzukiSang-Gun RohMDPI AGarticlemammary glandepithelial barriermastitischemerinVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3194, p 3194 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mammary gland epithelial barrier mastitis chemerin Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	mammary gland epithelial barrier mastitis chemerin Veterinary medicine SF600-1100 Zoology QL1-991 Yutaka Suzuki Sachi Chiba Koki Nishihara Keiichi Nakajima Akihiko Hagino Won-Seob Kim Hong-Gu Lee Tomonori Nochi Toru Suzuki Sang-Gun Roh Chemerin Regulates Epithelial Barrier Function of Mammary Glands in Dairy Cows
description	Epithelial barrier function in the mammary gland acts as a forefront of the defense mechanism against mastitis, which is widespread and a major disorder in dairy production. Chemerin is a chemoattractant protein with potent antimicrobial ability, but its role in the mammary gland remains unelucidated. The aim of this study was to determine the function of chemerin in mammary epithelial tissue of dairy cows in lactation or dry-off periods. Mammary epithelial cells produced chemerin protein, and secreted chemerin was detected in milk samples. Chemerin treatment promoted the proliferation of cultured bovine mammary epithelial cells and protected the integrity of the epithelial cell layer from hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-induced damage. Meanwhile, chemerin levels were higher in mammary tissue with mastitis. Tumor necrosis factor alpha (TNF-α) strongly upregulated the expression of the chemerin-coding gene (<i>RARRES2</i>) in mammary epithelial cells. Therefore, chemerin was suggested to support mammary epithelial cell growth and epithelial barrier function and to be regulated by inflammatory stimuli. Our results may indicate chemerin as a novel therapeutic target for diseases in the bovine mammary gland.
format	article
author	Yutaka Suzuki Sachi Chiba Koki Nishihara Keiichi Nakajima Akihiko Hagino Won-Seob Kim Hong-Gu Lee Tomonori Nochi Toru Suzuki Sang-Gun Roh
author_facet	Yutaka Suzuki Sachi Chiba Koki Nishihara Keiichi Nakajima Akihiko Hagino Won-Seob Kim Hong-Gu Lee Tomonori Nochi Toru Suzuki Sang-Gun Roh
author_sort	Yutaka Suzuki
title	Chemerin Regulates Epithelial Barrier Function of Mammary Glands in Dairy Cows
title_short	Chemerin Regulates Epithelial Barrier Function of Mammary Glands in Dairy Cows
title_full	Chemerin Regulates Epithelial Barrier Function of Mammary Glands in Dairy Cows
title_fullStr	Chemerin Regulates Epithelial Barrier Function of Mammary Glands in Dairy Cows
title_full_unstemmed	Chemerin Regulates Epithelial Barrier Function of Mammary Glands in Dairy Cows
title_sort	chemerin regulates epithelial barrier function of mammary glands in dairy cows
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8baa7f87c1dc41c88f9bce5a70c5f683
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Chemerin Regulates Epithelial Barrier Function of Mammary Glands in Dairy Cows

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