Human ex vivo lung perfusion: a novel model to study human lung diseases

Abstract Experimental animal models to predict physiological responses to injury and stress in humans have inherent limitations. Therefore, the development of preclinical human models is of paramount importance. Ex vivo lung perfusion (EVLP) has typically been used to recondition donor lungs before...

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Autores principales:	Nayra Cárdenes, John Sembrat, Kentaro Noda, Tyler Lovelace, Diana Álvarez, Humberto E. Trejo Bittar, Brian J. Philips, Mehdi Nouraie, Panayiotis V. Benos, Pablo G. Sánchez, Mauricio Rojas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8bc473a935524370bc235806f5fd1ab2
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spelling	oai:doaj.org-article:8bc473a935524370bc235806f5fd1ab22021-12-02T14:01:36ZHuman ex vivo lung perfusion: a novel model to study human lung diseases10.1038/s41598-020-79434-42045-2322https://doaj.org/article/8bc473a935524370bc235806f5fd1ab22021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79434-4https://doaj.org/toc/2045-2322Abstract Experimental animal models to predict physiological responses to injury and stress in humans have inherent limitations. Therefore, the development of preclinical human models is of paramount importance. Ex vivo lung perfusion (EVLP) has typically been used to recondition donor lungs before transplantation. However, this technique has recently advanced into a model to emulate lung mechanics and physiology during injury. In the present study, we propose that the EVLP of diseased human lungs is a well-suited preclinical model for translational research on chronic lung diseases. Throughout this paper, we demonstrate this technique's feasibility in pulmonary arterial hypertension (PAH), idiopathic pulmonary fibrosis (IPF), emphysema, and non-disease donor lungs not suitable for transplantation. In this study, we aimed to perfuse the lungs for 6 h with the EVLP system. This facilitated a robust and continuous assessment of airway mechanics, pulmonary hemodynamics, gas exchange, and biochemical parameters. We then collected at different time points tissue biopsies of lung parenchyma to isolate RNA and DNA to identify each disease's unique gene expression. Thus, demonstrating that EVLP could successfully serve as a clinically relevant experimental model to derive essential insights into pulmonary pathophysiology and various human lung diseases.Nayra CárdenesJohn SembratKentaro NodaTyler LovelaceDiana ÁlvarezHumberto E. Trejo BittarBrian J. PhilipsMehdi NouraiePanayiotis V. BenosPablo G. SánchezMauricio RojasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nayra Cárdenes John Sembrat Kentaro Noda Tyler Lovelace Diana Álvarez Humberto E. Trejo Bittar Brian J. Philips Mehdi Nouraie Panayiotis V. Benos Pablo G. Sánchez Mauricio Rojas Human ex vivo lung perfusion: a novel model to study human lung diseases
description	Abstract Experimental animal models to predict physiological responses to injury and stress in humans have inherent limitations. Therefore, the development of preclinical human models is of paramount importance. Ex vivo lung perfusion (EVLP) has typically been used to recondition donor lungs before transplantation. However, this technique has recently advanced into a model to emulate lung mechanics and physiology during injury. In the present study, we propose that the EVLP of diseased human lungs is a well-suited preclinical model for translational research on chronic lung diseases. Throughout this paper, we demonstrate this technique's feasibility in pulmonary arterial hypertension (PAH), idiopathic pulmonary fibrosis (IPF), emphysema, and non-disease donor lungs not suitable for transplantation. In this study, we aimed to perfuse the lungs for 6 h with the EVLP system. This facilitated a robust and continuous assessment of airway mechanics, pulmonary hemodynamics, gas exchange, and biochemical parameters. We then collected at different time points tissue biopsies of lung parenchyma to isolate RNA and DNA to identify each disease's unique gene expression. Thus, demonstrating that EVLP could successfully serve as a clinically relevant experimental model to derive essential insights into pulmonary pathophysiology and various human lung diseases.
format	article
author	Nayra Cárdenes John Sembrat Kentaro Noda Tyler Lovelace Diana Álvarez Humberto E. Trejo Bittar Brian J. Philips Mehdi Nouraie Panayiotis V. Benos Pablo G. Sánchez Mauricio Rojas
author_facet	Nayra Cárdenes John Sembrat Kentaro Noda Tyler Lovelace Diana Álvarez Humberto E. Trejo Bittar Brian J. Philips Mehdi Nouraie Panayiotis V. Benos Pablo G. Sánchez Mauricio Rojas
author_sort	Nayra Cárdenes
title	Human ex vivo lung perfusion: a novel model to study human lung diseases
title_short	Human ex vivo lung perfusion: a novel model to study human lung diseases
title_full	Human ex vivo lung perfusion: a novel model to study human lung diseases
title_fullStr	Human ex vivo lung perfusion: a novel model to study human lung diseases
title_full_unstemmed	Human ex vivo lung perfusion: a novel model to study human lung diseases
title_sort	human ex vivo lung perfusion: a novel model to study human lung diseases
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8bc473a935524370bc235806f5fd1ab2
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Human ex vivo lung perfusion: a novel model to study human lung diseases

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