Quantification of blood-brain barrier permeability by dynamic contrast-enhanced NIRS

Abstract The blood-brain barrier (BBB) is integral to maintaining a suitable microenvironment for neurons to function properly. Despite its importance, there are no bedside methods of assessing BBB disruption to help guide management of critical-care patients. The aim of this study was to demonstrat...

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Autores principales:	Daniel Milej, Androu Abdalmalak, Lise Desjardins, Hassaan Ahmed, Ting-Yim Lee, Mamadou Diop, Keith St. Lawrence
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8bcb374eb5d340feabc0fbbe40b6cbf1
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spelling	oai:doaj.org-article:8bcb374eb5d340feabc0fbbe40b6cbf12021-12-02T16:08:23ZQuantification of blood-brain barrier permeability by dynamic contrast-enhanced NIRS10.1038/s41598-017-01922-x2045-2322https://doaj.org/article/8bcb374eb5d340feabc0fbbe40b6cbf12017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01922-xhttps://doaj.org/toc/2045-2322Abstract The blood-brain barrier (BBB) is integral to maintaining a suitable microenvironment for neurons to function properly. Despite its importance, there are no bedside methods of assessing BBB disruption to help guide management of critical-care patients. The aim of this study was to demonstrate that dynamic contrast-enhanced (DCE) near-infrared spectroscopy (NIRS) can quantify the permeability surface-area product (PS) of the BBB. Experiments were conducted in rats in which the BBB was opened by image-guided focused ultrasound. DCE-NIRS data were acquired with two dyes of different molecular weight, indocyanine green (ICG, 67 kDa) and 800CW carboxylate (IRDye, 1166 Da), and PS maps were generated by DCE computer tomography (CT) for comparison. Both dyes showed a strong correlation between measured PS values and sonication power (R2 = 0.95 and 0.92 for ICG and IRDye respectively), and the PS values for IRDye were in good agreement with CT values obtained with a contrast agent of similar molecular weight. These proof-of-principle experiments demonstrate that DCE NIRS can quantify BBB permeability. The next step in translating this method to critical care practice will be to adapt depth sensitive methods to minimize the effects of scalp contamination on NIRS PS values.Daniel MilejAndrou AbdalmalakLise DesjardinsHassaan AhmedTing-Yim LeeMamadou DiopKeith St. LawrenceNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel Milej Androu Abdalmalak Lise Desjardins Hassaan Ahmed Ting-Yim Lee Mamadou Diop Keith St. Lawrence Quantification of blood-brain barrier permeability by dynamic contrast-enhanced NIRS
description	Abstract The blood-brain barrier (BBB) is integral to maintaining a suitable microenvironment for neurons to function properly. Despite its importance, there are no bedside methods of assessing BBB disruption to help guide management of critical-care patients. The aim of this study was to demonstrate that dynamic contrast-enhanced (DCE) near-infrared spectroscopy (NIRS) can quantify the permeability surface-area product (PS) of the BBB. Experiments were conducted in rats in which the BBB was opened by image-guided focused ultrasound. DCE-NIRS data were acquired with two dyes of different molecular weight, indocyanine green (ICG, 67 kDa) and 800CW carboxylate (IRDye, 1166 Da), and PS maps were generated by DCE computer tomography (CT) for comparison. Both dyes showed a strong correlation between measured PS values and sonication power (R2 = 0.95 and 0.92 for ICG and IRDye respectively), and the PS values for IRDye were in good agreement with CT values obtained with a contrast agent of similar molecular weight. These proof-of-principle experiments demonstrate that DCE NIRS can quantify BBB permeability. The next step in translating this method to critical care practice will be to adapt depth sensitive methods to minimize the effects of scalp contamination on NIRS PS values.
format	article
author	Daniel Milej Androu Abdalmalak Lise Desjardins Hassaan Ahmed Ting-Yim Lee Mamadou Diop Keith St. Lawrence
author_facet	Daniel Milej Androu Abdalmalak Lise Desjardins Hassaan Ahmed Ting-Yim Lee Mamadou Diop Keith St. Lawrence
author_sort	Daniel Milej
title	Quantification of blood-brain barrier permeability by dynamic contrast-enhanced NIRS
title_short	Quantification of blood-brain barrier permeability by dynamic contrast-enhanced NIRS
title_full	Quantification of blood-brain barrier permeability by dynamic contrast-enhanced NIRS
title_fullStr	Quantification of blood-brain barrier permeability by dynamic contrast-enhanced NIRS
title_full_unstemmed	Quantification of blood-brain barrier permeability by dynamic contrast-enhanced NIRS
title_sort	quantification of blood-brain barrier permeability by dynamic contrast-enhanced nirs
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8bcb374eb5d340feabc0fbbe40b6cbf1
work_keys_str_mv	AT danielmilej quantificationofbloodbrainbarrierpermeabilitybydynamiccontrastenhancednirs AT androuabdalmalak quantificationofbloodbrainbarrierpermeabilitybydynamiccontrastenhancednirs AT lisedesjardins quantificationofbloodbrainbarrierpermeabilitybydynamiccontrastenhancednirs AT hassaanahmed quantificationofbloodbrainbarrierpermeabilitybydynamiccontrastenhancednirs AT tingyimlee quantificationofbloodbrainbarrierpermeabilitybydynamiccontrastenhancednirs AT mamadoudiop quantificationofbloodbrainbarrierpermeabilitybydynamiccontrastenhancednirs AT keithstlawrence quantificationofbloodbrainbarrierpermeabilitybydynamiccontrastenhancednirs
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Quantification of blood-brain barrier permeability by dynamic contrast-enhanced NIRS

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