Metabolic networks for nitrogen utilization in Prevotella ruminicola 23

Abstract Nitrogen metabolism in gut systems remains poorly studied in spite of its importance for microbial growth and its implications for the metabolism of the host. Prevotella spp. are the most predominant bacteria detected in the rumen, but their presence has also been related to health and dise...

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Autores principales:	Jong Nam Kim, Celia Méndez–García, Renae R. Geier, Michael Iakiviak, Jongsoo Chang, Isaac Cann, Roderick I. Mackie
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8bd04f9327034f31b269a90aea033b46
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spelling	oai:doaj.org-article:8bd04f9327034f31b269a90aea033b462021-12-02T16:06:01ZMetabolic networks for nitrogen utilization in Prevotella ruminicola 2310.1038/s41598-017-08463-32045-2322https://doaj.org/article/8bd04f9327034f31b269a90aea033b462017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08463-3https://doaj.org/toc/2045-2322Abstract Nitrogen metabolism in gut systems remains poorly studied in spite of its importance for microbial growth and its implications for the metabolism of the host. Prevotella spp. are the most predominant bacteria detected in the rumen, but their presence has also been related to health and disease states in the human gut and oral cavity. To explore the metabolic networks for nitrogen assimilation in this bacterium, changes in gene expression profiles in response to variations in the available nitrogen source and to different concentrations of ammonium were analyzed by microarray and reverse transcription quantitative PCR, and linked with function by further proteomic analysis. The observed patterns of transcript abundances for genes involved in ammonium assimilation differed from the classical “enteric paradigm” for nitrogen utilization. Expression of genes encoding high substrate affinity nitrogen assimilation enzymes (GS-GOGAT system) was similar in growth-limiting and non-limiting nitrogen concentrations in P. ruminicola 23, whereas E. coli and Salmonella spp. responses to excess nitrogen involve only low substrate affinity enzymes. This versatile behavior might be a key feature for ecological success in habitats such as the rumen and human colon where nitrogen is rarely limiting for growth, and might be linked to previously reported Prevotella spp. population imbalances relative to other bacterial species in gut systems.Jong Nam KimCelia Méndez–GarcíaRenae R. GeierMichael IakiviakJongsoo ChangIsaac CannRoderick I. MackieNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jong Nam Kim Celia Méndez–García Renae R. Geier Michael Iakiviak Jongsoo Chang Isaac Cann Roderick I. Mackie Metabolic networks for nitrogen utilization in Prevotella ruminicola 23
description	Abstract Nitrogen metabolism in gut systems remains poorly studied in spite of its importance for microbial growth and its implications for the metabolism of the host. Prevotella spp. are the most predominant bacteria detected in the rumen, but their presence has also been related to health and disease states in the human gut and oral cavity. To explore the metabolic networks for nitrogen assimilation in this bacterium, changes in gene expression profiles in response to variations in the available nitrogen source and to different concentrations of ammonium were analyzed by microarray and reverse transcription quantitative PCR, and linked with function by further proteomic analysis. The observed patterns of transcript abundances for genes involved in ammonium assimilation differed from the classical “enteric paradigm” for nitrogen utilization. Expression of genes encoding high substrate affinity nitrogen assimilation enzymes (GS-GOGAT system) was similar in growth-limiting and non-limiting nitrogen concentrations in P. ruminicola 23, whereas E. coli and Salmonella spp. responses to excess nitrogen involve only low substrate affinity enzymes. This versatile behavior might be a key feature for ecological success in habitats such as the rumen and human colon where nitrogen is rarely limiting for growth, and might be linked to previously reported Prevotella spp. population imbalances relative to other bacterial species in gut systems.
format	article
author	Jong Nam Kim Celia Méndez–García Renae R. Geier Michael Iakiviak Jongsoo Chang Isaac Cann Roderick I. Mackie
author_facet	Jong Nam Kim Celia Méndez–García Renae R. Geier Michael Iakiviak Jongsoo Chang Isaac Cann Roderick I. Mackie
author_sort	Jong Nam Kim
title	Metabolic networks for nitrogen utilization in Prevotella ruminicola 23
title_short	Metabolic networks for nitrogen utilization in Prevotella ruminicola 23
title_full	Metabolic networks for nitrogen utilization in Prevotella ruminicola 23
title_fullStr	Metabolic networks for nitrogen utilization in Prevotella ruminicola 23
title_full_unstemmed	Metabolic networks for nitrogen utilization in Prevotella ruminicola 23
title_sort	metabolic networks for nitrogen utilization in prevotella ruminicola 23
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8bd04f9327034f31b269a90aea033b46
work_keys_str_mv	AT jongnamkim metabolicnetworksfornitrogenutilizationinprevotellaruminicola23 AT celiamendezgarcia metabolicnetworksfornitrogenutilizationinprevotellaruminicola23 AT renaergeier metabolicnetworksfornitrogenutilizationinprevotellaruminicola23 AT michaeliakiviak metabolicnetworksfornitrogenutilizationinprevotellaruminicola23 AT jongsoochang metabolicnetworksfornitrogenutilizationinprevotellaruminicola23 AT isaaccann metabolicnetworksfornitrogenutilizationinprevotellaruminicola23 AT roderickimackie metabolicnetworksfornitrogenutilizationinprevotellaruminicola23
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Metabolic networks for nitrogen utilization in Prevotella ruminicola 23

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