Antibacterial activity of endophytic fungi in Pometia pinnata against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus

Abstract. Endophytic fungi associated with medicinal plants are able to produce bioactive compounds which potentially contain medicinal ingredients. Matoa (Pometia pinnata J. R. Forst & G. Forst) is one of Indonesian medicinal plants. This research aims to test antibacterial activities of ethyl...

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Autores principales:	Ratna Setyaningsih, Ari Susilowati, Dina Prasetyani
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8be446cf6f3048b7aea359e64c22ef52
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spelling	oai:doaj.org-article:8be446cf6f3048b7aea359e64c22ef522021-11-22T00:44:11ZAntibacterial activity of endophytic fungi in Pometia pinnata against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus1412-033X2085-472210.13057/biodiv/d211148https://doaj.org/article/8be446cf6f3048b7aea359e64c22ef522020-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5842https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Endophytic fungi associated with medicinal plants are able to produce bioactive compounds which potentially contain medicinal ingredients. Matoa (Pometia pinnata J. R. Forst & G. Forst) is one of Indonesian medicinal plants. This research aims to test antibacterial activities of ethyl acetate extract of matoa’s endophytic fungi to Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA) and also identify isolates having high antibacterial activity. Initial test on antibacterial activity was conducted by using paper disc diffusion, while advanced test was conducted by determining minimum inhibitory concentration (MIC). The fungi were identified in morphological and molecular manner with amplification and sequencing of ITS region. Bacterial cells exposed to the extract were observed by using scanning electron microscope (SEM). Isolate AM72 was found out to have the highest antibacterial activity compared to other 12 isolates tested, with inhibition zone diameter of 31.57 mm to S. aureus and 22.87 mm to MRSA. Isolate AM72 was identified as Lasiodiplodia theobromae. Isolate BMB identified as Aspergillus oryzae produced inhibition zone with 21.96 mm diameter to S. aureus and 21.50 mm diameter to MRSA. Advanced test to isolate AM72 gave the same MIC results to S. aureus and MRSA of 1.56%. Antibacterial compound produced by isolate AM72 were found out to be able to damage bacterial cell walls. Keywords: Antibacterial, Endohpytic Fungi, Matoa, S. aureus, MRSARatna SetyaningsihAri SusilowatiDina PrasetyaniMBI & UNS SoloarticleBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Ratna Setyaningsih Ari Susilowati Dina Prasetyani Antibacterial activity of endophytic fungi in Pometia pinnata against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus
description	Abstract. Endophytic fungi associated with medicinal plants are able to produce bioactive compounds which potentially contain medicinal ingredients. Matoa (Pometia pinnata J. R. Forst & G. Forst) is one of Indonesian medicinal plants. This research aims to test antibacterial activities of ethyl acetate extract of matoa’s endophytic fungi to Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA) and also identify isolates having high antibacterial activity. Initial test on antibacterial activity was conducted by using paper disc diffusion, while advanced test was conducted by determining minimum inhibitory concentration (MIC). The fungi were identified in morphological and molecular manner with amplification and sequencing of ITS region. Bacterial cells exposed to the extract were observed by using scanning electron microscope (SEM). Isolate AM72 was found out to have the highest antibacterial activity compared to other 12 isolates tested, with inhibition zone diameter of 31.57 mm to S. aureus and 22.87 mm to MRSA. Isolate AM72 was identified as Lasiodiplodia theobromae. Isolate BMB identified as Aspergillus oryzae produced inhibition zone with 21.96 mm diameter to S. aureus and 21.50 mm diameter to MRSA. Advanced test to isolate AM72 gave the same MIC results to S. aureus and MRSA of 1.56%. Antibacterial compound produced by isolate AM72 were found out to be able to damage bacterial cell walls. Keywords: Antibacterial, Endohpytic Fungi, Matoa, S. aureus, MRSA
format	article
author	Ratna Setyaningsih Ari Susilowati Dina Prasetyani
author_facet	Ratna Setyaningsih Ari Susilowati Dina Prasetyani
author_sort	Ratna Setyaningsih
title	Antibacterial activity of endophytic fungi in Pometia pinnata against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus
title_short	Antibacterial activity of endophytic fungi in Pometia pinnata against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus
title_full	Antibacterial activity of endophytic fungi in Pometia pinnata against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus
title_fullStr	Antibacterial activity of endophytic fungi in Pometia pinnata against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus
title_full_unstemmed	Antibacterial activity of endophytic fungi in Pometia pinnata against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus
title_sort	antibacterial activity of endophytic fungi in pometia pinnata against staphylococcus aureus and methicillin-resistant staphylococcus aureus
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/8be446cf6f3048b7aea359e64c22ef52
work_keys_str_mv	AT ratnasetyaningsih antibacterialactivityofendophyticfungiinpometiapinnataagainststaphylococcusaureusandmethicillinresistantstaphylococcusaureus AT arisusilowati antibacterialactivityofendophyticfungiinpometiapinnataagainststaphylococcusaureusandmethicillinresistantstaphylococcusaureus AT dinaprasetyani antibacterialactivityofendophyticfungiinpometiapinnataagainststaphylococcusaureusandmethicillinresistantstaphylococcusaureus
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Antibacterial activity of endophytic fungi in Pometia pinnata against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus

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