Inactivation of the htpsA gene affects capsule development and pathogenicity of Streptococcus suis

Streptococcus suis serotype 2 (S. suis 2) is an important swine pathogen and also an emerging zoonotic agent. HtpsA has been reported as an immunogenic cell surface protein on the bacterium. In the present study, we constructed an isogenic mutant strain of htpsA, namely ΔhtpsA, to study its role in...

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Autores principales:	Hua Ni, Min Li, Qiaoqiao Wang, Jing Wang, Xumiao Liu, Feng Zheng, Dan Hu, Xu Yu, Yifang Han, Qi Zhang, Tingting Zhou, Yiwen Wang, Chunhui Wang, Jimin Gao, Zhu-Qing Shao, Xiuzhen Pan
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	streptococcus suis serotype 2 histidine triad proteins gene knockout bacterial virulence capsule development Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/8be8e939be1c4fef915909926f4951a0
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spelling	oai:doaj.org-article:8be8e939be1c4fef915909926f4951a02021-11-17T14:21:58ZInactivation of the htpsA gene affects capsule development and pathogenicity of Streptococcus suis2150-55942150-560810.1080/21505594.2020.1792080https://doaj.org/article/8be8e939be1c4fef915909926f4951a02020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1792080https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Streptococcus suis serotype 2 (S. suis 2) is an important swine pathogen and also an emerging zoonotic agent. HtpsA has been reported as an immunogenic cell surface protein on the bacterium. In the present study, we constructed an isogenic mutant strain of htpsA, namely ΔhtpsA, to study its role in the development and virulence of S. suis 2. Our results showed that the mutant strain lost its typical encapsulated structure with decreased concentrations of sialic acid. Furthermore, the survival rate in whole blood, the anti-phagocytosis by RAW264.7 murine macrophage, and the adherence ability to HEp-2 cells were all significantly affected in the ΔhtpsA. In addition, the deletion of htpsA sharply attenuated the virulence of S. suis 2 in an infection model of mouse. RNA-seq analysis revealed that 126 genes were differentially expressed between the ΔhtpsA and the wild-type strains, including 28 upregulated and 98 downregulated genes. Among the downregulated genes, many were involved in carbohydrate metabolism and synthesis of virulence-associated factors. Taken together, htpsA was demonstrated to play a role in the morphological development and pathogenesis of the highly virulent S. suis 2 05ZYH33 strain.Hua NiMin LiQiaoqiao WangJing WangXumiao LiuFeng ZhengDan HuXu YuYifang HanQi ZhangTingting ZhouYiwen WangChunhui WangJimin GaoZhu-Qing ShaoXiuzhen PanTaylor & Francis Grouparticlestreptococcus suis serotype 2histidine triad proteinsgene knockoutbacterial virulencecapsule developmentInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 927-940 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	streptococcus suis serotype 2 histidine triad proteins gene knockout bacterial virulence capsule development Infectious and parasitic diseases RC109-216
spellingShingle	streptococcus suis serotype 2 histidine triad proteins gene knockout bacterial virulence capsule development Infectious and parasitic diseases RC109-216 Hua Ni Min Li Qiaoqiao Wang Jing Wang Xumiao Liu Feng Zheng Dan Hu Xu Yu Yifang Han Qi Zhang Tingting Zhou Yiwen Wang Chunhui Wang Jimin Gao Zhu-Qing Shao Xiuzhen Pan Inactivation of the htpsA gene affects capsule development and pathogenicity of Streptococcus suis
description	Streptococcus suis serotype 2 (S. suis 2) is an important swine pathogen and also an emerging zoonotic agent. HtpsA has been reported as an immunogenic cell surface protein on the bacterium. In the present study, we constructed an isogenic mutant strain of htpsA, namely ΔhtpsA, to study its role in the development and virulence of S. suis 2. Our results showed that the mutant strain lost its typical encapsulated structure with decreased concentrations of sialic acid. Furthermore, the survival rate in whole blood, the anti-phagocytosis by RAW264.7 murine macrophage, and the adherence ability to HEp-2 cells were all significantly affected in the ΔhtpsA. In addition, the deletion of htpsA sharply attenuated the virulence of S. suis 2 in an infection model of mouse. RNA-seq analysis revealed that 126 genes were differentially expressed between the ΔhtpsA and the wild-type strains, including 28 upregulated and 98 downregulated genes. Among the downregulated genes, many were involved in carbohydrate metabolism and synthesis of virulence-associated factors. Taken together, htpsA was demonstrated to play a role in the morphological development and pathogenesis of the highly virulent S. suis 2 05ZYH33 strain.
format	article
author	Hua Ni Min Li Qiaoqiao Wang Jing Wang Xumiao Liu Feng Zheng Dan Hu Xu Yu Yifang Han Qi Zhang Tingting Zhou Yiwen Wang Chunhui Wang Jimin Gao Zhu-Qing Shao Xiuzhen Pan
author_facet	Hua Ni Min Li Qiaoqiao Wang Jing Wang Xumiao Liu Feng Zheng Dan Hu Xu Yu Yifang Han Qi Zhang Tingting Zhou Yiwen Wang Chunhui Wang Jimin Gao Zhu-Qing Shao Xiuzhen Pan
author_sort	Hua Ni
title	Inactivation of the htpsA gene affects capsule development and pathogenicity of Streptococcus suis
title_short	Inactivation of the htpsA gene affects capsule development and pathogenicity of Streptococcus suis
title_full	Inactivation of the htpsA gene affects capsule development and pathogenicity of Streptococcus suis
title_fullStr	Inactivation of the htpsA gene affects capsule development and pathogenicity of Streptococcus suis
title_full_unstemmed	Inactivation of the htpsA gene affects capsule development and pathogenicity of Streptococcus suis
title_sort	inactivation of the htpsa gene affects capsule development and pathogenicity of streptococcus suis
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/8be8e939be1c4fef915909926f4951a0
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Inactivation of the htpsA gene affects capsule development and pathogenicity of Streptococcus suis

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