Panel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma

Abstract Early-stage diagnosis of pancreatic ductal adenocarcinoma (PDAC) is difficult due to non-specific symptoms. Circulating miRNAs in body fluids have been emerging as potential non-invasive biomarkers for diagnosis of many cancers. Thus, this study aimed to assess a panel of miRNAs for their a...

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Autores principales:	Imteyaz Ahmad Khan, Safoora Rashid, Nidhi Singh, Sumaira Rashid, Vishwajeet Singh, Deepak Gunjan, Prasenjit Das, Nihar Ranjan Dash, Ravindra Mohan Pandey, Shyam Singh Chauhan, Surabhi Gupta, Anoop Saraya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8bea46440b1f4d2797704d8d7e3530c4
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spelling	oai:doaj.org-article:8bea46440b1f4d2797704d8d7e3530c42021-12-02T10:44:15ZPanel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma10.1038/s41598-021-82266-52045-2322https://doaj.org/article/8bea46440b1f4d2797704d8d7e3530c42021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82266-5https://doaj.org/toc/2045-2322Abstract Early-stage diagnosis of pancreatic ductal adenocarcinoma (PDAC) is difficult due to non-specific symptoms. Circulating miRNAs in body fluids have been emerging as potential non-invasive biomarkers for diagnosis of many cancers. Thus, this study aimed to assess a panel of miRNAs for their ability to differentiate PDAC from chronic pancreatitis (CP), a benign inflammatory condition of the pancreas. Next-generation sequencing was performed to identify miRNAs present in 60 FFPE tissue samples (27 PDAC, 23 CP and 10 normal pancreatic tissues). Four up-regulated miRNAs (miR-215-5p, miR-122-5p, miR-192-5p, and miR-181a-2-3p) and four down-regulated miRNAs (miR-30b-5p, miR-216b-5p, miR-320b, and miR-214-5p) in PDAC compared to CP were selected based on next-generation sequencing results. The levels of these 8 differentially expressed miRNAs were measured by qRT-PCR in 125 serum samples (50 PDAC, 50 CP, and 25 healthy controls (HC)). The results showed significant upregulation of miR-215-5p, miR-122-5p, and miR-192-5p in PDAC serum samples. In contrast, levels of miR-30b-5p and miR-320b were significantly lower in PDAC as compared to CP and HC. ROC analysis showed that these 5 miRNAs can distinguish PDAC from both CP and HC. Hence, this panel can serve as a non-invasive biomarker for the early detection of PDAC.Imteyaz Ahmad KhanSafoora RashidNidhi SinghSumaira RashidVishwajeet SinghDeepak GunjanPrasenjit DasNihar Ranjan DashRavindra Mohan PandeyShyam Singh ChauhanSurabhi GuptaAnoop SarayaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Imteyaz Ahmad Khan Safoora Rashid Nidhi Singh Sumaira Rashid Vishwajeet Singh Deepak Gunjan Prasenjit Das Nihar Ranjan Dash Ravindra Mohan Pandey Shyam Singh Chauhan Surabhi Gupta Anoop Saraya Panel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma
description	Abstract Early-stage diagnosis of pancreatic ductal adenocarcinoma (PDAC) is difficult due to non-specific symptoms. Circulating miRNAs in body fluids have been emerging as potential non-invasive biomarkers for diagnosis of many cancers. Thus, this study aimed to assess a panel of miRNAs for their ability to differentiate PDAC from chronic pancreatitis (CP), a benign inflammatory condition of the pancreas. Next-generation sequencing was performed to identify miRNAs present in 60 FFPE tissue samples (27 PDAC, 23 CP and 10 normal pancreatic tissues). Four up-regulated miRNAs (miR-215-5p, miR-122-5p, miR-192-5p, and miR-181a-2-3p) and four down-regulated miRNAs (miR-30b-5p, miR-216b-5p, miR-320b, and miR-214-5p) in PDAC compared to CP were selected based on next-generation sequencing results. The levels of these 8 differentially expressed miRNAs were measured by qRT-PCR in 125 serum samples (50 PDAC, 50 CP, and 25 healthy controls (HC)). The results showed significant upregulation of miR-215-5p, miR-122-5p, and miR-192-5p in PDAC serum samples. In contrast, levels of miR-30b-5p and miR-320b were significantly lower in PDAC as compared to CP and HC. ROC analysis showed that these 5 miRNAs can distinguish PDAC from both CP and HC. Hence, this panel can serve as a non-invasive biomarker for the early detection of PDAC.
format	article
author	Imteyaz Ahmad Khan Safoora Rashid Nidhi Singh Sumaira Rashid Vishwajeet Singh Deepak Gunjan Prasenjit Das Nihar Ranjan Dash Ravindra Mohan Pandey Shyam Singh Chauhan Surabhi Gupta Anoop Saraya
author_facet	Imteyaz Ahmad Khan Safoora Rashid Nidhi Singh Sumaira Rashid Vishwajeet Singh Deepak Gunjan Prasenjit Das Nihar Ranjan Dash Ravindra Mohan Pandey Shyam Singh Chauhan Surabhi Gupta Anoop Saraya
author_sort	Imteyaz Ahmad Khan
title	Panel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma
title_short	Panel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma
title_full	Panel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma
title_fullStr	Panel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma
title_full_unstemmed	Panel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma
title_sort	panel of serum mirnas as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8bea46440b1f4d2797704d8d7e3530c4
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Panel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma

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