Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian <i>Piper betle</i> L. Variants

The differences in pungency of “sirih” imply the probable occurrence of several variants of <i>Piper betle</i> L. in Malaysia. However, the metabolite profiles underlying the pungency of the different variants remain a subject of further research. The differences in metabolite profiles o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Muhamad Faris Osman, Soo Yee Lee, Shahrul Razid Sarbini, Siti Munirah Mohd Faudzi, Shamsul Khamis, Badrul Hisyam Zainudin, Khozirah Shaari
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Piper betle</i> L. <i>Piper rubro-venosum</i> hort. ex Rodigas <sup>1</sup>H-NMR GC-MS PCA essential oils Botany QK1-989
Acceso en línea:	https://doaj.org/article/8beb64f01b554e0da801019e23b77db6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8beb64f01b554e0da801019e23b77db6
record_format	dspace
spelling	oai:doaj.org-article:8beb64f01b554e0da801019e23b77db62021-11-25T18:47:12ZMetabolomics-Driven Discovery of an Introduced Species and Two Malaysian <i>Piper betle</i> L. Variants10.3390/plants101125102223-7747https://doaj.org/article/8beb64f01b554e0da801019e23b77db62021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2510https://doaj.org/toc/2223-7747The differences in pungency of “sirih” imply the probable occurrence of several variants of <i>Piper betle</i> L. in Malaysia. However, the metabolite profiles underlying the pungency of the different variants remain a subject of further research. The differences in metabolite profiles of selected Malaysian <i>P. betle</i> variants were thus investigated; specifically, the leaf aqueous methanolic extracts and essential oils were analyzed via <sup>1</sup>H-NMR and GC-MS metabolomics, respectively. Principal component analysis (PCA) of the <sup>1</sup>H-NMR spectral data showed quantitative differences in the metabolite profiles of “sirih melayu” and “sirih india” and revealed an ambiguous group of samples with low acetic acid content, which was identified as <i>Piper rubro-venosum</i> hort. ex Rodigas based on DNA sequences of the internal transcribed spacer 2 (ITS2) region. The finding was supported by PCA of two GC-MS datasets of <i>P. betle</i> samples obtained from several states in Peninsular Malaysia, which displayed clustering of the samples into “sirih melayu” and “sirih india” groups. Higher abundance of chavicol acetate was consistently found to be characteristic of “sirih melayu”. The present research has provided preliminary evidence supporting the notion of occurrence of two <i>P. betle</i> variants in Malaysia based on chemical profiles, which may be related to the different genders of <i>P. betle</i>.Muhamad Faris OsmanSoo Yee LeeShahrul Razid SarbiniSiti Munirah Mohd FaudziShamsul KhamisBadrul Hisyam ZainudinKhozirah ShaariMDPI AGarticle<i>Piper betle</i> L.<i>Piper rubro-venosum</i> hort. ex Rodigas<sup>1</sup>H-NMRGC-MSPCAessential oilsBotanyQK1-989ENPlants, Vol 10, Iss 2510, p 2510 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Piper betle</i> L. <i>Piper rubro-venosum</i> hort. ex Rodigas <sup>1</sup>H-NMR GC-MS PCA essential oils Botany QK1-989
spellingShingle	<i>Piper betle</i> L. <i>Piper rubro-venosum</i> hort. ex Rodigas <sup>1</sup>H-NMR GC-MS PCA essential oils Botany QK1-989 Muhamad Faris Osman Soo Yee Lee Shahrul Razid Sarbini Siti Munirah Mohd Faudzi Shamsul Khamis Badrul Hisyam Zainudin Khozirah Shaari Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian <i>Piper betle</i> L. Variants
description	The differences in pungency of “sirih” imply the probable occurrence of several variants of <i>Piper betle</i> L. in Malaysia. However, the metabolite profiles underlying the pungency of the different variants remain a subject of further research. The differences in metabolite profiles of selected Malaysian <i>P. betle</i> variants were thus investigated; specifically, the leaf aqueous methanolic extracts and essential oils were analyzed via <sup>1</sup>H-NMR and GC-MS metabolomics, respectively. Principal component analysis (PCA) of the <sup>1</sup>H-NMR spectral data showed quantitative differences in the metabolite profiles of “sirih melayu” and “sirih india” and revealed an ambiguous group of samples with low acetic acid content, which was identified as <i>Piper rubro-venosum</i> hort. ex Rodigas based on DNA sequences of the internal transcribed spacer 2 (ITS2) region. The finding was supported by PCA of two GC-MS datasets of <i>P. betle</i> samples obtained from several states in Peninsular Malaysia, which displayed clustering of the samples into “sirih melayu” and “sirih india” groups. Higher abundance of chavicol acetate was consistently found to be characteristic of “sirih melayu”. The present research has provided preliminary evidence supporting the notion of occurrence of two <i>P. betle</i> variants in Malaysia based on chemical profiles, which may be related to the different genders of <i>P. betle</i>.
format	article
author	Muhamad Faris Osman Soo Yee Lee Shahrul Razid Sarbini Siti Munirah Mohd Faudzi Shamsul Khamis Badrul Hisyam Zainudin Khozirah Shaari
author_facet	Muhamad Faris Osman Soo Yee Lee Shahrul Razid Sarbini Siti Munirah Mohd Faudzi Shamsul Khamis Badrul Hisyam Zainudin Khozirah Shaari
author_sort	Muhamad Faris Osman
title	Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian <i>Piper betle</i> L. Variants
title_short	Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian <i>Piper betle</i> L. Variants
title_full	Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian <i>Piper betle</i> L. Variants
title_fullStr	Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian <i>Piper betle</i> L. Variants
title_full_unstemmed	Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian <i>Piper betle</i> L. Variants
title_sort	metabolomics-driven discovery of an introduced species and two malaysian <i>piper betle</i> l. variants
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8beb64f01b554e0da801019e23b77db6
work_keys_str_mv	AT muhamadfarisosman metabolomicsdrivendiscoveryofanintroducedspeciesandtwomalaysianipiperbetleilvariants AT sooyeelee metabolomicsdrivendiscoveryofanintroducedspeciesandtwomalaysianipiperbetleilvariants AT shahrulrazidsarbini metabolomicsdrivendiscoveryofanintroducedspeciesandtwomalaysianipiperbetleilvariants AT sitimunirahmohdfaudzi metabolomicsdrivendiscoveryofanintroducedspeciesandtwomalaysianipiperbetleilvariants AT shamsulkhamis metabolomicsdrivendiscoveryofanintroducedspeciesandtwomalaysianipiperbetleilvariants AT badrulhisyamzainudin metabolomicsdrivendiscoveryofanintroducedspeciesandtwomalaysianipiperbetleilvariants AT khozirahshaari metabolomicsdrivendiscoveryofanintroducedspeciesandtwomalaysianipiperbetleilvariants
_version_	1718410724232921088

Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian <i>Piper betle</i> L. Variants

Ejemplares similares