MCT1 and MCT4 Expression and Lactate Flux Activity Increase During White and Brown Adipogenesis and Impact Adipocyte Metabolism

Abstract Adipose tissue takes up glucose and releases lactate, thereby contributing significantly to systemic glucose and lactate homeostasis. This implies the necessity of upregulation of net acid and lactate flux capacity during adipocyte differentiation and function. However, the regulation of la...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Charlotte Petersen, Mette D. Nielsen, Elise S. Andersen, Astrid L. Basse, Marie S. Isidor, Lasse K. Markussen, Birgitte M. Viuff, Ian H. Lambert, Jacob B. Hansen, Stine F. Pedersen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8bf3e0267a624fd6a5bb3adcd9b1f5ba
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8bf3e0267a624fd6a5bb3adcd9b1f5ba
record_format	dspace
spelling	oai:doaj.org-article:8bf3e0267a624fd6a5bb3adcd9b1f5ba2021-12-02T15:06:00ZMCT1 and MCT4 Expression and Lactate Flux Activity Increase During White and Brown Adipogenesis and Impact Adipocyte Metabolism10.1038/s41598-017-13298-z2045-2322https://doaj.org/article/8bf3e0267a624fd6a5bb3adcd9b1f5ba2017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-13298-zhttps://doaj.org/toc/2045-2322Abstract Adipose tissue takes up glucose and releases lactate, thereby contributing significantly to systemic glucose and lactate homeostasis. This implies the necessity of upregulation of net acid and lactate flux capacity during adipocyte differentiation and function. However, the regulation of lactate- and acid/base transporters in adipocytes is poorly understood. Here, we tested the hypothesis that adipocyte thermogenesis, browning and differentiation are associated with an upregulation of plasma membrane lactate and acid/base transport capacity that in turn is important for adipocyte metabolism. The mRNA and protein levels of the lactate-H+ transporter MCT1 and the Na+,HCO3 − cotransporter NBCe1 were upregulated in mouse interscapular brown and inguinal white adipose tissue upon cold induction of thermogenesis and browning. MCT1, MCT4, and NBCe1 were furthermore strongly upregulated at the mRNA and protein level upon differentiation of cultured pre-adipocytes. Adipocyte differentiation was accompanied by increased plasma membrane lactate flux capacity, which was reduced by MCT inhibition and by MCT1 knockdown. Finally, in differentiated brown adipocytes, glycolysis (assessed as ECAR), and after noradrenergic stimulation also oxidative metabolism (OCR), was decreased by MCT inhibition. We suggest that upregulation of MCT1- and MCT4-mediated lactate flux capacity and NBCe1-mediated HCO3 −/pH homeostasis are important for the physiological function of mature adipocytes.Charlotte PetersenMette D. NielsenElise S. AndersenAstrid L. BasseMarie S. IsidorLasse K. MarkussenBirgitte M. ViuffIan H. LambertJacob B. HansenStine F. PedersenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Charlotte Petersen Mette D. Nielsen Elise S. Andersen Astrid L. Basse Marie S. Isidor Lasse K. Markussen Birgitte M. Viuff Ian H. Lambert Jacob B. Hansen Stine F. Pedersen MCT1 and MCT4 Expression and Lactate Flux Activity Increase During White and Brown Adipogenesis and Impact Adipocyte Metabolism
description	Abstract Adipose tissue takes up glucose and releases lactate, thereby contributing significantly to systemic glucose and lactate homeostasis. This implies the necessity of upregulation of net acid and lactate flux capacity during adipocyte differentiation and function. However, the regulation of lactate- and acid/base transporters in adipocytes is poorly understood. Here, we tested the hypothesis that adipocyte thermogenesis, browning and differentiation are associated with an upregulation of plasma membrane lactate and acid/base transport capacity that in turn is important for adipocyte metabolism. The mRNA and protein levels of the lactate-H+ transporter MCT1 and the Na+,HCO3 − cotransporter NBCe1 were upregulated in mouse interscapular brown and inguinal white adipose tissue upon cold induction of thermogenesis and browning. MCT1, MCT4, and NBCe1 were furthermore strongly upregulated at the mRNA and protein level upon differentiation of cultured pre-adipocytes. Adipocyte differentiation was accompanied by increased plasma membrane lactate flux capacity, which was reduced by MCT inhibition and by MCT1 knockdown. Finally, in differentiated brown adipocytes, glycolysis (assessed as ECAR), and after noradrenergic stimulation also oxidative metabolism (OCR), was decreased by MCT inhibition. We suggest that upregulation of MCT1- and MCT4-mediated lactate flux capacity and NBCe1-mediated HCO3 −/pH homeostasis are important for the physiological function of mature adipocytes.
format	article
author	Charlotte Petersen Mette D. Nielsen Elise S. Andersen Astrid L. Basse Marie S. Isidor Lasse K. Markussen Birgitte M. Viuff Ian H. Lambert Jacob B. Hansen Stine F. Pedersen
author_facet	Charlotte Petersen Mette D. Nielsen Elise S. Andersen Astrid L. Basse Marie S. Isidor Lasse K. Markussen Birgitte M. Viuff Ian H. Lambert Jacob B. Hansen Stine F. Pedersen
author_sort	Charlotte Petersen
title	MCT1 and MCT4 Expression and Lactate Flux Activity Increase During White and Brown Adipogenesis and Impact Adipocyte Metabolism
title_short	MCT1 and MCT4 Expression and Lactate Flux Activity Increase During White and Brown Adipogenesis and Impact Adipocyte Metabolism
title_full	MCT1 and MCT4 Expression and Lactate Flux Activity Increase During White and Brown Adipogenesis and Impact Adipocyte Metabolism
title_fullStr	MCT1 and MCT4 Expression and Lactate Flux Activity Increase During White and Brown Adipogenesis and Impact Adipocyte Metabolism
title_full_unstemmed	MCT1 and MCT4 Expression and Lactate Flux Activity Increase During White and Brown Adipogenesis and Impact Adipocyte Metabolism
title_sort	mct1 and mct4 expression and lactate flux activity increase during white and brown adipogenesis and impact adipocyte metabolism
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8bf3e0267a624fd6a5bb3adcd9b1f5ba
work_keys_str_mv	AT charlottepetersen mct1andmct4expressionandlactatefluxactivityincreaseduringwhiteandbrownadipogenesisandimpactadipocytemetabolism AT mettednielsen mct1andmct4expressionandlactatefluxactivityincreaseduringwhiteandbrownadipogenesisandimpactadipocytemetabolism AT elisesandersen mct1andmct4expressionandlactatefluxactivityincreaseduringwhiteandbrownadipogenesisandimpactadipocytemetabolism AT astridlbasse mct1andmct4expressionandlactatefluxactivityincreaseduringwhiteandbrownadipogenesisandimpactadipocytemetabolism AT mariesisidor mct1andmct4expressionandlactatefluxactivityincreaseduringwhiteandbrownadipogenesisandimpactadipocytemetabolism AT lassekmarkussen mct1andmct4expressionandlactatefluxactivityincreaseduringwhiteandbrownadipogenesisandimpactadipocytemetabolism AT birgittemviuff mct1andmct4expressionandlactatefluxactivityincreaseduringwhiteandbrownadipogenesisandimpactadipocytemetabolism AT ianhlambert mct1andmct4expressionandlactatefluxactivityincreaseduringwhiteandbrownadipogenesisandimpactadipocytemetabolism AT jacobbhansen mct1andmct4expressionandlactatefluxactivityincreaseduringwhiteandbrownadipogenesisandimpactadipocytemetabolism AT stinefpedersen mct1andmct4expressionandlactatefluxactivityincreaseduringwhiteandbrownadipogenesisandimpactadipocytemetabolism
_version_	1718388656558833664

MCT1 and MCT4 Expression and Lactate Flux Activity Increase During White and Brown Adipogenesis and Impact Adipocyte Metabolism

Ejemplares similares