Exposure of stevia (Stevia rebaudiana B.) to silver nanoparticles in vitro: transport and accumulation

Abstract The impact of nanotechnology in the field of agricultural sciences creates the need to study in greater detail the effect of products offering nanoparticles for application in plant species of agricultural interest. The objective of this study was to determine the response of stevia (Stevia...

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Autores principales:	Celia G. Castro-González, Lino Sánchez-Segura, Fernando C. Gómez-Merino, Jericó J. Bello-Bello
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8bfa58745e9e4a98ba4e9b8a75d0bee0
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spelling	oai:doaj.org-article:8bfa58745e9e4a98ba4e9b8a75d0bee02021-12-02T15:09:31ZExposure of stevia (Stevia rebaudiana B.) to silver nanoparticles in vitro: transport and accumulation10.1038/s41598-019-46828-y2045-2322https://doaj.org/article/8bfa58745e9e4a98ba4e9b8a75d0bee02019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46828-yhttps://doaj.org/toc/2045-2322Abstract The impact of nanotechnology in the field of agricultural sciences creates the need to study in greater detail the effect of products offering nanoparticles for application in plant species of agricultural interest. The objective of this study was to determine the response of stevia (Stevia rebaudiana B.) in vitro to different concentrations of AgNPs (silver nanoparticles), as well as to characterize and identify their absorption, translocation and accumulation mechanisms. Nodal segments of stevia grown in MS medium supplemented with AgNPs (0,12.5, 25, 50,100 and 200 mg L−1) were used. After 30 days of in vitro shoot proliferation, the number of shoots per explant, shoot length, chlorophyll content, dry matter content and the metallic silver (Ag) content of the plants were quantified. In addition, characterization, transport and accumulation of silver nanoparticles were performed by microscopic analysis. AgNPs were shown to be present in epidermal stem cells, within vascular bundles and in intermembrane spaces. In leaves, they were observed in ribs and stomata. The current and future use of AgNPs in agricultural sciences opens up the possibility of studying their effects on different plant species.Celia G. Castro-GonzálezLino Sánchez-SeguraFernando C. Gómez-MerinoJericó J. Bello-BelloNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Celia G. Castro-González Lino Sánchez-Segura Fernando C. Gómez-Merino Jericó J. Bello-Bello Exposure of stevia (Stevia rebaudiana B.) to silver nanoparticles in vitro: transport and accumulation
description	Abstract The impact of nanotechnology in the field of agricultural sciences creates the need to study in greater detail the effect of products offering nanoparticles for application in plant species of agricultural interest. The objective of this study was to determine the response of stevia (Stevia rebaudiana B.) in vitro to different concentrations of AgNPs (silver nanoparticles), as well as to characterize and identify their absorption, translocation and accumulation mechanisms. Nodal segments of stevia grown in MS medium supplemented with AgNPs (0,12.5, 25, 50,100 and 200 mg L−1) were used. After 30 days of in vitro shoot proliferation, the number of shoots per explant, shoot length, chlorophyll content, dry matter content and the metallic silver (Ag) content of the plants were quantified. In addition, characterization, transport and accumulation of silver nanoparticles were performed by microscopic analysis. AgNPs were shown to be present in epidermal stem cells, within vascular bundles and in intermembrane spaces. In leaves, they were observed in ribs and stomata. The current and future use of AgNPs in agricultural sciences opens up the possibility of studying their effects on different plant species.
format	article
author	Celia G. Castro-González Lino Sánchez-Segura Fernando C. Gómez-Merino Jericó J. Bello-Bello
author_facet	Celia G. Castro-González Lino Sánchez-Segura Fernando C. Gómez-Merino Jericó J. Bello-Bello
author_sort	Celia G. Castro-González
title	Exposure of stevia (Stevia rebaudiana B.) to silver nanoparticles in vitro: transport and accumulation
title_short	Exposure of stevia (Stevia rebaudiana B.) to silver nanoparticles in vitro: transport and accumulation
title_full	Exposure of stevia (Stevia rebaudiana B.) to silver nanoparticles in vitro: transport and accumulation
title_fullStr	Exposure of stevia (Stevia rebaudiana B.) to silver nanoparticles in vitro: transport and accumulation
title_full_unstemmed	Exposure of stevia (Stevia rebaudiana B.) to silver nanoparticles in vitro: transport and accumulation
title_sort	exposure of stevia (stevia rebaudiana b.) to silver nanoparticles in vitro: transport and accumulation
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/8bfa58745e9e4a98ba4e9b8a75d0bee0
work_keys_str_mv	AT celiagcastrogonzalez exposureofsteviasteviarebaudianabtosilvernanoparticlesinvitrotransportandaccumulation AT linosanchezsegura exposureofsteviasteviarebaudianabtosilvernanoparticlesinvitrotransportandaccumulation AT fernandocgomezmerino exposureofsteviasteviarebaudianabtosilvernanoparticlesinvitrotransportandaccumulation AT jericojbellobello exposureofsteviasteviarebaudianabtosilvernanoparticlesinvitrotransportandaccumulation
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Exposure of stevia (Stevia rebaudiana B.) to silver nanoparticles in vitro: transport and accumulation

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