Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal5

The biosynthesis of exopolysaccharides (EPSs) is essential for endophytic bacterial colonisation in plants bacause this exopolymer both protects bacterial cells against the defence and oxidative systems of plants and acts on the plant colonisation mechanism in <i>Gluconacetobacter diazotrophic...

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Autores principales:	Katyanne Wanderley, Dayse Sousa, Gabriel Silva, Josemir Maia, Maria Silva, Marcia Vidal, José Baldani, Carlos Meneses
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	plant growth-promoting bacteria exopolymer production bacterial protein phosphorylation autophosphorylation Science Q
Acceso en línea:	https://doaj.org/article/8c00169c9bea4d34802a7546bfe55913
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spelling	oai:doaj.org-article:8c00169c9bea4d34802a7546bfe559132021-11-25T18:11:23ZTyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal510.3390/life111112312075-1729https://doaj.org/article/8c00169c9bea4d34802a7546bfe559132021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1231https://doaj.org/toc/2075-1729The biosynthesis of exopolysaccharides (EPSs) is essential for endophytic bacterial colonisation in plants bacause this exopolymer both protects bacterial cells against the defence and oxidative systems of plants and acts on the plant colonisation mechanism in <i>Gluconacetobacter diazotrophicus</i>. The pathway involved in the biosynthesis of bacterial EPS has not been fully elucidated, and several areas related to its molecular regulation mechanisms are still lacking. <i>G. diazotrophicus</i> relies heavily on EPS for survival indirectly by protecting plants from pathogen attack as well as for endophytic maintenance and adhesion in plant tissues. Here, we report that EPS from <i>G. diazotrophicus</i> strain Pal5 is a signal polymer that controls its own biosynthesis. EPS production depends on a bacterial tyrosine (BY) kinase (Wzc) that consists of a component that is able to phosphorylate a glycosyltranferase or to self-phosphorylate. EPS interacts with the extracellular domain of Wzc, which regulates kinase activity. In <i>G. diazotrophicus</i> strains that are deficient in EPS production, the Wzc is rendered inoperative by self-phosphorylation. The presence of EPS promotes the phosphorylation of a glycosyltransferase in the pathway, thus producing EPS. Wzc-mediated self-regulation is an attribute for the control of exopolysaccharide biosynthesis in <i>G. diazotrophicus</i>.Katyanne WanderleyDayse SousaGabriel SilvaJosemir MaiaMaria SilvaMarcia VidalJosé BaldaniCarlos MenesesMDPI AGarticleplant growth-promoting bacteriaexopolymer productionbacterial protein phosphorylationautophosphorylationScienceQENLife, Vol 11, Iss 1231, p 1231 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	plant growth-promoting bacteria exopolymer production bacterial protein phosphorylation autophosphorylation Science Q
spellingShingle	plant growth-promoting bacteria exopolymer production bacterial protein phosphorylation autophosphorylation Science Q Katyanne Wanderley Dayse Sousa Gabriel Silva Josemir Maia Maria Silva Marcia Vidal José Baldani Carlos Meneses Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal5
description	The biosynthesis of exopolysaccharides (EPSs) is essential for endophytic bacterial colonisation in plants bacause this exopolymer both protects bacterial cells against the defence and oxidative systems of plants and acts on the plant colonisation mechanism in <i>Gluconacetobacter diazotrophicus</i>. The pathway involved in the biosynthesis of bacterial EPS has not been fully elucidated, and several areas related to its molecular regulation mechanisms are still lacking. <i>G. diazotrophicus</i> relies heavily on EPS for survival indirectly by protecting plants from pathogen attack as well as for endophytic maintenance and adhesion in plant tissues. Here, we report that EPS from <i>G. diazotrophicus</i> strain Pal5 is a signal polymer that controls its own biosynthesis. EPS production depends on a bacterial tyrosine (BY) kinase (Wzc) that consists of a component that is able to phosphorylate a glycosyltranferase or to self-phosphorylate. EPS interacts with the extracellular domain of Wzc, which regulates kinase activity. In <i>G. diazotrophicus</i> strains that are deficient in EPS production, the Wzc is rendered inoperative by self-phosphorylation. The presence of EPS promotes the phosphorylation of a glycosyltransferase in the pathway, thus producing EPS. Wzc-mediated self-regulation is an attribute for the control of exopolysaccharide biosynthesis in <i>G. diazotrophicus</i>.
format	article
author	Katyanne Wanderley Dayse Sousa Gabriel Silva Josemir Maia Maria Silva Marcia Vidal José Baldani Carlos Meneses
author_facet	Katyanne Wanderley Dayse Sousa Gabriel Silva Josemir Maia Maria Silva Marcia Vidal José Baldani Carlos Meneses
author_sort	Katyanne Wanderley
title	Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal5
title_short	Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal5
title_full	Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal5
title_fullStr	Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal5
title_full_unstemmed	Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal5
title_sort	tyrosine kinase self-phosphorylation controls exopolysaccharide biosynthesis in <i>gluconacetobacter diazotrophicus</i> strain pal5
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8c00169c9bea4d34802a7546bfe55913
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Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in <i>Gluconacetobacter diazotrophicus</i> Strain Pal5

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