Drosophila dyskerin is required for somatic stem cell homeostasis

Abstract Drosophila represents an excellent model to dissect the roles played by the evolutionary conserved family of eukaryotic dyskerins. These multifunctional proteins are involved in the formation of H/ACA snoRNP and telomerase complexes, both involved in essential cellular tasks. Since fly telo...

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Autores principales:	Rosario Vicidomini, Arianna Petrizzo, Annamaria di Giovanni, Laura Cassese, Antonella Anna Lombardi, Caterina Pragliola, Maria Furia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8c04359702d440e4b65297dc67c379d7
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spelling	oai:doaj.org-article:8c04359702d440e4b65297dc67c379d72021-12-02T12:32:33ZDrosophila dyskerin is required for somatic stem cell homeostasis10.1038/s41598-017-00446-82045-2322https://doaj.org/article/8c04359702d440e4b65297dc67c379d72017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00446-8https://doaj.org/toc/2045-2322Abstract Drosophila represents an excellent model to dissect the roles played by the evolutionary conserved family of eukaryotic dyskerins. These multifunctional proteins are involved in the formation of H/ACA snoRNP and telomerase complexes, both involved in essential cellular tasks. Since fly telomere integrity is guaranteed by a different mechanism, we used this organism to investigate the specific role played by dyskerin in somatic stem cell maintenance. To this aim, we focussed on Drosophila midgut, a hierarchically organized and well characterized model for stemness analysis. Surprisingly, the ubiquitous loss of the protein uniquely affects the formation of the larval stem cell niches, without altering other midgut cell types. The number of adult midgut precursor stem cells is dramatically reduced, and this effect is not caused by premature differentiation and is cell-autonomous. Moreover, a few dispersed precursors found in the depleted midguts can maintain stem identity and the ability to divide asymmetrically, nor show cell-growth defects or undergo apoptosis. Instead, their loss is mainly specifically dependent on defective amplification. These studies establish a strict link between dyskerin and somatic stem cell maintenance in a telomerase-lacking organism, indicating that loss of stemness can be regarded as a conserved, telomerase-independent effect of dyskerin dysfunction.Rosario VicidominiArianna PetrizzoAnnamaria di GiovanniLaura CasseseAntonella Anna LombardiCaterina PragliolaMaria FuriaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rosario Vicidomini Arianna Petrizzo Annamaria di Giovanni Laura Cassese Antonella Anna Lombardi Caterina Pragliola Maria Furia Drosophila dyskerin is required for somatic stem cell homeostasis
description	Abstract Drosophila represents an excellent model to dissect the roles played by the evolutionary conserved family of eukaryotic dyskerins. These multifunctional proteins are involved in the formation of H/ACA snoRNP and telomerase complexes, both involved in essential cellular tasks. Since fly telomere integrity is guaranteed by a different mechanism, we used this organism to investigate the specific role played by dyskerin in somatic stem cell maintenance. To this aim, we focussed on Drosophila midgut, a hierarchically organized and well characterized model for stemness analysis. Surprisingly, the ubiquitous loss of the protein uniquely affects the formation of the larval stem cell niches, without altering other midgut cell types. The number of adult midgut precursor stem cells is dramatically reduced, and this effect is not caused by premature differentiation and is cell-autonomous. Moreover, a few dispersed precursors found in the depleted midguts can maintain stem identity and the ability to divide asymmetrically, nor show cell-growth defects or undergo apoptosis. Instead, their loss is mainly specifically dependent on defective amplification. These studies establish a strict link between dyskerin and somatic stem cell maintenance in a telomerase-lacking organism, indicating that loss of stemness can be regarded as a conserved, telomerase-independent effect of dyskerin dysfunction.
format	article
author	Rosario Vicidomini Arianna Petrizzo Annamaria di Giovanni Laura Cassese Antonella Anna Lombardi Caterina Pragliola Maria Furia
author_facet	Rosario Vicidomini Arianna Petrizzo Annamaria di Giovanni Laura Cassese Antonella Anna Lombardi Caterina Pragliola Maria Furia
author_sort	Rosario Vicidomini
title	Drosophila dyskerin is required for somatic stem cell homeostasis
title_short	Drosophila dyskerin is required for somatic stem cell homeostasis
title_full	Drosophila dyskerin is required for somatic stem cell homeostasis
title_fullStr	Drosophila dyskerin is required for somatic stem cell homeostasis
title_full_unstemmed	Drosophila dyskerin is required for somatic stem cell homeostasis
title_sort	drosophila dyskerin is required for somatic stem cell homeostasis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8c04359702d440e4b65297dc67c379d7
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Drosophila dyskerin is required for somatic stem cell homeostasis

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