Direct supercritical angle localization microscopy for nanometer 3D superresolution

Supercritical angle localisation microscopy (SALM) allows the z-positions of single fluorophores to be extracted from the intensity of supercritical angle fluorescence. Here the authors improve the z-resolution of SALM, and report nanometre isotropic localisation precision on DNA origami structures.

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Autores principales:	Anindita Dasgupta, Joran Deschamps, Ulf Matti, Uwe Hübner, Jan Becker, Sebastian Strauss, Ralf Jungmann, Rainer Heintzmann, Jonas Ries
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/8c05c23b63364577bddb90ef5a6f0f6d
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spelling	oai:doaj.org-article:8c05c23b63364577bddb90ef5a6f0f6d2021-12-02T10:54:03ZDirect supercritical angle localization microscopy for nanometer 3D superresolution10.1038/s41467-021-21333-x2041-1723https://doaj.org/article/8c05c23b63364577bddb90ef5a6f0f6d2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21333-xhttps://doaj.org/toc/2041-1723Supercritical angle localisation microscopy (SALM) allows the z-positions of single fluorophores to be extracted from the intensity of supercritical angle fluorescence. Here the authors improve the z-resolution of SALM, and report nanometre isotropic localisation precision on DNA origami structures.Anindita DasguptaJoran DeschampsUlf MattiUwe HübnerJan BeckerSebastian StraussRalf JungmannRainer HeintzmannJonas RiesNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Anindita Dasgupta Joran Deschamps Ulf Matti Uwe Hübner Jan Becker Sebastian Strauss Ralf Jungmann Rainer Heintzmann Jonas Ries Direct supercritical angle localization microscopy for nanometer 3D superresolution
description	Supercritical angle localisation microscopy (SALM) allows the z-positions of single fluorophores to be extracted from the intensity of supercritical angle fluorescence. Here the authors improve the z-resolution of SALM, and report nanometre isotropic localisation precision on DNA origami structures.
format	article
author	Anindita Dasgupta Joran Deschamps Ulf Matti Uwe Hübner Jan Becker Sebastian Strauss Ralf Jungmann Rainer Heintzmann Jonas Ries
author_facet	Anindita Dasgupta Joran Deschamps Ulf Matti Uwe Hübner Jan Becker Sebastian Strauss Ralf Jungmann Rainer Heintzmann Jonas Ries
author_sort	Anindita Dasgupta
title	Direct supercritical angle localization microscopy for nanometer 3D superresolution
title_short	Direct supercritical angle localization microscopy for nanometer 3D superresolution
title_full	Direct supercritical angle localization microscopy for nanometer 3D superresolution
title_fullStr	Direct supercritical angle localization microscopy for nanometer 3D superresolution
title_full_unstemmed	Direct supercritical angle localization microscopy for nanometer 3D superresolution
title_sort	direct supercritical angle localization microscopy for nanometer 3d superresolution
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8c05c23b63364577bddb90ef5a6f0f6d
work_keys_str_mv	AT aninditadasgupta directsupercriticalanglelocalizationmicroscopyfornanometer3dsuperresolution AT jorandeschamps directsupercriticalanglelocalizationmicroscopyfornanometer3dsuperresolution AT ulfmatti directsupercriticalanglelocalizationmicroscopyfornanometer3dsuperresolution AT uwehubner directsupercriticalanglelocalizationmicroscopyfornanometer3dsuperresolution AT janbecker directsupercriticalanglelocalizationmicroscopyfornanometer3dsuperresolution AT sebastianstrauss directsupercriticalanglelocalizationmicroscopyfornanometer3dsuperresolution AT ralfjungmann directsupercriticalanglelocalizationmicroscopyfornanometer3dsuperresolution AT rainerheintzmann directsupercriticalanglelocalizationmicroscopyfornanometer3dsuperresolution AT jonasries directsupercriticalanglelocalizationmicroscopyfornanometer3dsuperresolution
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Direct supercritical angle localization microscopy for nanometer 3D superresolution

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