Evaluation of a mechanical lung model to test small animal whole body plethysmography

Abstract Whole-body plethysmography (WBP) is an established method to determine physiological parameters and pathophysiological alteration of breathing in animals and animal models of a variety of diseases. Although frequently used, there is ongoing debate about what exactly is measured by whole-bod...

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Autores principales:	Swen Hülsmann, Amara Khan, Liya Hagos, Martin Hindermann, Torsten Nägel, Christian Dullin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8c0777891b084aa79e14710b32cb0085
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spelling	oai:doaj.org-article:8c0777891b084aa79e14710b32cb00852021-12-02T15:09:23ZEvaluation of a mechanical lung model to test small animal whole body plethysmography10.1038/s41598-021-96355-y2045-2322https://doaj.org/article/8c0777891b084aa79e14710b32cb00852021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96355-yhttps://doaj.org/toc/2045-2322Abstract Whole-body plethysmography (WBP) is an established method to determine physiological parameters and pathophysiological alteration of breathing in animals and animal models of a variety of diseases. Although frequently used, there is ongoing debate about what exactly is measured by whole-body-plethysmography and how reliable the data derived from this method are. Here, we designed an artificial lung model that enables a thorough evaluation of different predictions about and around whole-body plethysmography. Using our lung model, we confirmed that during WBP two components contribute to the pressure changes detected in the chamber: (1) the increase in the pressure due to heating and moistening of the air during inspiration, termed conditioning; (2) changes in the chamber pressure that depend on airway resistance. Both components overlap and contribute to the temporal pressure-profile measured in the chamber or across the wall of the chamber, respectively. Our data showed that a precise measurement of the breathing volume appears to be hindered by at least two factors: (1) the unknown relative contribution of each of these two components; (2) not only the air in the inspired volume is conditioned during inspiration, but also air within the residual volume and dead space that is recruited during inspiration. Moreover, our data suggest that the expiratory negative pressure peak that is used to determine the enhanced pause (Penh) parameter is not a measure for airway resistance as such but rather a consequence of the animal’s response to the airway resistance, using forced or active expiration to overcome the resistance by a higher thoracic pressure.Swen HülsmannAmara KhanLiya HagosMartin HindermannTorsten NägelChristian DullinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Swen Hülsmann Amara Khan Liya Hagos Martin Hindermann Torsten Nägel Christian Dullin Evaluation of a mechanical lung model to test small animal whole body plethysmography
description	Abstract Whole-body plethysmography (WBP) is an established method to determine physiological parameters and pathophysiological alteration of breathing in animals and animal models of a variety of diseases. Although frequently used, there is ongoing debate about what exactly is measured by whole-body-plethysmography and how reliable the data derived from this method are. Here, we designed an artificial lung model that enables a thorough evaluation of different predictions about and around whole-body plethysmography. Using our lung model, we confirmed that during WBP two components contribute to the pressure changes detected in the chamber: (1) the increase in the pressure due to heating and moistening of the air during inspiration, termed conditioning; (2) changes in the chamber pressure that depend on airway resistance. Both components overlap and contribute to the temporal pressure-profile measured in the chamber or across the wall of the chamber, respectively. Our data showed that a precise measurement of the breathing volume appears to be hindered by at least two factors: (1) the unknown relative contribution of each of these two components; (2) not only the air in the inspired volume is conditioned during inspiration, but also air within the residual volume and dead space that is recruited during inspiration. Moreover, our data suggest that the expiratory negative pressure peak that is used to determine the enhanced pause (Penh) parameter is not a measure for airway resistance as such but rather a consequence of the animal’s response to the airway resistance, using forced or active expiration to overcome the resistance by a higher thoracic pressure.
format	article
author	Swen Hülsmann Amara Khan Liya Hagos Martin Hindermann Torsten Nägel Christian Dullin
author_facet	Swen Hülsmann Amara Khan Liya Hagos Martin Hindermann Torsten Nägel Christian Dullin
author_sort	Swen Hülsmann
title	Evaluation of a mechanical lung model to test small animal whole body plethysmography
title_short	Evaluation of a mechanical lung model to test small animal whole body plethysmography
title_full	Evaluation of a mechanical lung model to test small animal whole body plethysmography
title_fullStr	Evaluation of a mechanical lung model to test small animal whole body plethysmography
title_full_unstemmed	Evaluation of a mechanical lung model to test small animal whole body plethysmography
title_sort	evaluation of a mechanical lung model to test small animal whole body plethysmography
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8c0777891b084aa79e14710b32cb0085
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Evaluation of a mechanical lung model to test small animal whole body plethysmography

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