Inhibition of highly pathogenic avian influenza (HPAI) virus by a peptide derived from vFLIP through its direct destabilization of viruses

Abstract The antiviral activities of synthesized Kα2-helix peptide, which was derived from the viral FLICE-like inhibitor protein (vFLIP) of Kaposi’s sarcoma-associated herpesvirus (KSHV), against influenza A virus (IAV) were investigated in vitro and in vivo, and mechanisms of action were suggested...

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Autores principales:	Ho-Jin Moon, Chamilani Nikapitiya, Hyun-Cheol Lee, Min-Eun Park, Jae-Hoon Kim, Tae-Hwan Kim, Ji-Eun Yoon, Won-Kyung Cho, Jin Yeul Ma, Chul-Joong Kim, Jae U. Jung, Jong-Soo Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8c077a47490c45f891fa9dc38f80b75e
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spelling	oai:doaj.org-article:8c077a47490c45f891fa9dc38f80b75e2021-12-02T11:52:19ZInhibition of highly pathogenic avian influenza (HPAI) virus by a peptide derived from vFLIP through its direct destabilization of viruses10.1038/s41598-017-04777-42045-2322https://doaj.org/article/8c077a47490c45f891fa9dc38f80b75e2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04777-4https://doaj.org/toc/2045-2322Abstract The antiviral activities of synthesized Kα2-helix peptide, which was derived from the viral FLICE-like inhibitor protein (vFLIP) of Kaposi’s sarcoma-associated herpesvirus (KSHV), against influenza A virus (IAV) were investigated in vitro and in vivo, and mechanisms of action were suggested. In addition to the robust autophagy activity of the Kα2-helix peptide, the present study showed that treatment with the Kα2 peptide fused with the TAT peptide significantly inhibited IAV replication and transmission. Moreover, TAT-Kα2 peptide protected the mice, that were challenged with lethal doses of highly pathogenic influenza A H5N1 or H1N1 viruses. Mechanistically, we found that TAT-Kα2 peptide destabilized the viral membranes, depending on their lipid composition of the viral envelop. In addition to IAV, the Kα2 peptide inhibited infections with enveloped viruses, such as Vesicular Stomatitis Virus (VSV) and Respiratory Syncytial Virus (RSV), without cytotoxicity. These results suggest that TAT-Kα2 peptide is a potential antiviral agent for controlling emerging or re-emerging enveloped viruses, particularly diverse subtypes of IAVs.Ho-Jin MoonChamilani NikapitiyaHyun-Cheol LeeMin-Eun ParkJae-Hoon KimTae-Hwan KimJi-Eun YoonWon-Kyung ChoJin Yeul MaChul-Joong KimJae U. JungJong-Soo LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ho-Jin Moon Chamilani Nikapitiya Hyun-Cheol Lee Min-Eun Park Jae-Hoon Kim Tae-Hwan Kim Ji-Eun Yoon Won-Kyung Cho Jin Yeul Ma Chul-Joong Kim Jae U. Jung Jong-Soo Lee Inhibition of highly pathogenic avian influenza (HPAI) virus by a peptide derived from vFLIP through its direct destabilization of viruses
description	Abstract The antiviral activities of synthesized Kα2-helix peptide, which was derived from the viral FLICE-like inhibitor protein (vFLIP) of Kaposi’s sarcoma-associated herpesvirus (KSHV), against influenza A virus (IAV) were investigated in vitro and in vivo, and mechanisms of action were suggested. In addition to the robust autophagy activity of the Kα2-helix peptide, the present study showed that treatment with the Kα2 peptide fused with the TAT peptide significantly inhibited IAV replication and transmission. Moreover, TAT-Kα2 peptide protected the mice, that were challenged with lethal doses of highly pathogenic influenza A H5N1 or H1N1 viruses. Mechanistically, we found that TAT-Kα2 peptide destabilized the viral membranes, depending on their lipid composition of the viral envelop. In addition to IAV, the Kα2 peptide inhibited infections with enveloped viruses, such as Vesicular Stomatitis Virus (VSV) and Respiratory Syncytial Virus (RSV), without cytotoxicity. These results suggest that TAT-Kα2 peptide is a potential antiviral agent for controlling emerging or re-emerging enveloped viruses, particularly diverse subtypes of IAVs.
format	article
author	Ho-Jin Moon Chamilani Nikapitiya Hyun-Cheol Lee Min-Eun Park Jae-Hoon Kim Tae-Hwan Kim Ji-Eun Yoon Won-Kyung Cho Jin Yeul Ma Chul-Joong Kim Jae U. Jung Jong-Soo Lee
author_facet	Ho-Jin Moon Chamilani Nikapitiya Hyun-Cheol Lee Min-Eun Park Jae-Hoon Kim Tae-Hwan Kim Ji-Eun Yoon Won-Kyung Cho Jin Yeul Ma Chul-Joong Kim Jae U. Jung Jong-Soo Lee
author_sort	Ho-Jin Moon
title	Inhibition of highly pathogenic avian influenza (HPAI) virus by a peptide derived from vFLIP through its direct destabilization of viruses
title_short	Inhibition of highly pathogenic avian influenza (HPAI) virus by a peptide derived from vFLIP through its direct destabilization of viruses
title_full	Inhibition of highly pathogenic avian influenza (HPAI) virus by a peptide derived from vFLIP through its direct destabilization of viruses
title_fullStr	Inhibition of highly pathogenic avian influenza (HPAI) virus by a peptide derived from vFLIP through its direct destabilization of viruses
title_full_unstemmed	Inhibition of highly pathogenic avian influenza (HPAI) virus by a peptide derived from vFLIP through its direct destabilization of viruses
title_sort	inhibition of highly pathogenic avian influenza (hpai) virus by a peptide derived from vflip through its direct destabilization of viruses
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8c077a47490c45f891fa9dc38f80b75e
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Inhibition of highly pathogenic avian influenza (HPAI) virus by a peptide derived from vFLIP through its direct destabilization of viruses

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